This work was designed to study the free and immobilized partial purified lovastatin in various applications. The results of HMG-CoA reductase inhibition showed enzyme inhibition at 10 mM of standard and partial purified lovastatin with specific activity 0.056 and 0.062 U/mg protein respectively, compared with specific activity 0.277 U/mg protein without inhibitor. The results of the thermal stability and storage time on lovastatin for inhibition of HMG-CoA reductase demonstrated that the standard and partial purified lovastatin were stabled in t

This study was aimed to investigate the antiproliferative effect of lemongrass essential oil (LEO) from Cymbopogon citratus on A549 cell line and their effect on the expression of p53 gene in lung cancer cells. The LEO was extracted and anlaysed using GC-MS analysis, the cytotoxicity of LEO against lung cancer cells was examined using MMT assay and finally the expression of p53 was determined via qRT-PCR. The chromatogram of GC-MS exhibited 14 compounds in ELO, the major one is citral (56.4%). The cytotoxicity of LEO was dose dependent which proliferation of the lung cells were significantly inhibited with increased concentration of essential oil (P≤ 0.01) as well as the IC50 was 29.13 ppm. Furthermore

This study was aimed to investigate the involvement of Myeloid Related Protein 14 (MRP14) in the production of CXC Motif Chemokine Ligand 9 (CXCL9) in thyroiditis patients. The first group composed of 27 hypothyroidism (HO) patients (12 men and 15 women), age average about 46 years old. The second group had 43 patients (8 men and 35 women) with hyperthyroidism (HR), their mean of age was 42, while 30 healthy persons act as control group (HC) had an average age of 33.47 of men (13) and women (17). The three groups were testing for triiodothyronine (T3), thyroxine (T4) and thyroid stimulating hormone (TSH) furthermore, testing the thyroglobulin (TG), anti-thyroperoxidase (a-TPO), anti-TSH receptor (TSHR) and serum level of MRP14 by

The following study was carried out to investigate gene expressions of the CASPASE 9 gene and CASPASE 9 protein with the development of chronic myeloid leukemia (CML) in Iraq. The differences in the expression of this gene between patients and healthy controls were included. The correlation of age and gender with CML occurrence comparing with controls was studied. Results showed significant increases in the mean of gene expression level (ΔCt) of patient groups compared to the corresponding ΔCt means in control group, also the gene expression folding (2^-∆∆Ct) of  CASPASE 9  gene  reflect significant differences in the expression showed the highest level in CML patients which reached to (16.19), the

This study was aimed to detect the spreading of E.histolytica among children with diarrhea, to achieve that,221 stool specimens were collected from diarrheal children of all ages and both gender, in Baghdad city at a period extended from early September 2019 to the end of February 2020. The collected specimens were examined directly by the light microscope for detecting the presence of E.histolytica. DNA was extracted from positive microscopically stool specimens, then<

This study was designed to determine the effect of partial purified lovastatin on the lipid profile and liver functions in rabbits. The rabbits were divided into six groups, each group containing four rabbits, and the lipid profile and liver functions were checked at zero, 30, 60, and 90 days. The parameters of the cholesterol, high-density lipoprotein (HDL), triglycerides (TG), low-density lipoprotein (LDL), very- low-density lipoprotein (VLDL), blood bilirubin (BB), and liver enzymes (ALP, ALT and AST) were determined separately. Determination of parameters was made before treatment with any medication and after treatment with cholesterol (4 g/kilo/day), standard lovastatin (0.4 mg/kilo/day), cholesterol plus standard lovastati

Anise (Pimpinella anisum) is an annual herbaceous flowering plant, its widely used as spices, flavoring agent and fragrance. Anise seeds and its essential oils (EOs) has been used widely in the recipes of folk medicine in the Arab region, including Iraq. Therefore, the goal of this work was to extract and define the chemical compositions of aniseeds EOs, and study its antioxidant, antibacterial, and antibiofilm potentials. Aniseed EOs were extracted by hydro-distillation technique using Clevenger apparatus. Twenty-five distinct compounds have been recognized by Gas chromatographic–Mass spectral (GC-Mas) analysis, the major constituents were anisole (87

The current study was aimed to investigate the antibacterial and antibiofilm activity of ethanolic extract of Hibiscus sabdariffa L. calyx against locally isolated methicillin-resistant Staphylococcus aureus (MRSA) at different concentrations were started from 0.078 to 40 mg/ml. The extract was prepared by soaked calyces powder of Hibiscus sabdariffa L. with 80% ethanol in the Soxhlet extraction unit, and then it was aseptically filtered. The antibacterial activity was tested by agar diffusion method and broth microdilution method, this method was used to determine the minimum inhibitory concentration of extract, while the antibiofilm activity was determined by using 96-well polystyrene microtiter plate

The research was aimed to identify fruits morphological characteristics of 33 taxa belonging to the Lepidieae tribe from Brassicaceae. The characteristics of the fruit's general shape, apex shape, dimensions, colors, surface ornamentation, and beak were determined, the results indicated the importance of each of those characters in isolated species, In general, all the tribe's fruits were open capsule and short silicula. According to the dimensions of fruit, three species can be distinguished as a group with dimensions greater than 20mm, as in Brossardia, Coluteocarpus, and Didymophysa, and depending on the shape, it was possible to differentiate the species within this group, while the other species were less t

Dust storms are a common ecological occurrence in many world‘s countries, mainly in dry and semi-dry parts. Dust storms tremendously influence human health, the environment, the climate, and numerous social aspects. In this paper, spatial and temporal analysis, metrological triggers, and trajectory, dust exporting areas of a severe dust storm that occurred in Iraq on May 16, 2022, were investigated. The dust storm's backward trajectory was determined using HYSPLIT model, which is then compared with MODIS and Meteosat satellite images. The weather is then analyzed using the NCEP/NCAR Reanalysis model, and the approximate area of these sources was determined using Landsat 8 satellite image classification method. The results revealed

     CdSe/CdS Core/shell nanostructures were prepared through the chemical synthesis method. XRD ,FESEM and TEM investigations confirmed the formation of core/shell structure for the sample. The AFM measurement was employed to reveal the morphology of the prepared thin films. Optical characterizations of the quantum dots were done by UV-visible and photoluminescence spectra. It was found that the quantum dots prepared has  good optical properties. Due to the presence of shell coating on core CdSe, the energy gap of the core/shell nanomaterial were increased from 2.2 to 2.3eV. The resulted QDs  are a promising candidate for photovoltaic and biosensor applications.

One hundred forty three of Klebsiellapneumoniae isolates had been collected from some hospitals in Baghdad city. The isolates were taken from different clinical specimens.Antimicrobial susceptibility test was carried out towards fifteen antimicrobial agents by using Vitek2 system with Antimicrobial susceptibility test cards. The results of antibiogram showed that the local isolates were possess highly resistance towards most antimicrobial agents under study. The high resistance wastoAmpicillin while the low resistance was to Imipenem.Two methods were used for detection of Extended Spectrum Beta Lactamases (ESBLs) production; first methods by using of Vitek2 system,thesecondmethods by using of polymerase chain reaction (PCR) technique to dis

Anti-Toxoplasma gondii seropositive status among obese and diabetic patients has recently attracted substantial attention. The objective of this study was to assess the seropositive rate of T. gondii and its relation to high body mass index (BMI), diabetes, and metabolic syndrome among participants (n=100) who attended Al-Kadhimiya Teaching Hospital, Baghdad-Iraq. An observational analytical study was conducted from October 2019 to March 2020. Participants were divided into three groups based on their BMI; obese (n=38), overweight (n=32) and normal (n=30). In addition, they were divided into diabetic (n=45) and non-diabetic (n=55) based on clinical examination, laboratory examination, and medical interview. Another clas

This encapsulates the general relationship between plant and bacteria in the natural and agricultural ecosystem. It is based on the activities of useful bacteria, such as plant growth-promoting bacteria (PGPRs) and nitrogen-fixing bacteria, in promoting plant growth and plant tolerance to stressful situations regarding pollution, salinity, and drought. The article also mentions that the bacteria maintain plant health by secretion of phytohormones, nitrogen fixation, solubilization of phosphate, and production of antibiotics against pathogenic bacteria. The article also mentions the existing applications of the interaction in sustainable agriculture and bioremediation of contaminated soils.

Luminescent sensor membranes and sensor microplates are presented for continuous or high-throughput wide-range measurement of pH based on a europium probe.

The aim of this paper is to approximate multidimensional functions by using the type of Feedforward neural networks (FFNNs) which is called Greedy radial basis function neural networks (GRBFNNs). Also, we introduce a modification to the greedy algorithm which is used to train the greedy radial basis function neural networks. An error bound are introduced in Sobolev space. Finally, a comparison was made between the three algorithms (modified greedy algorithm, Backpropagation algorithm and the result is published in [16]).

One hundred isolates of Pseudomonas aeruginosa were obtained from patients admitted to Baghdad hospitals, Iraq during the period between May 2018 until July 2018. These isolates were distributed as 15 isolates from blood, 25 isolates from urinary tract infections, 10 isolates from sputum, 12 isolates from wounds, 15 isolates from ear infections, 15 isolates from bronchial wash of patients suffering from respiratory tract infections in addition to 8 isolates from cystic fibrosis patients. The isolates were initially identified by culturing on MacConkey agar, blood agar and P. aeruginosa agar then diagnosed by performing some morphological and biochemical tests. The second diagnosis was done by API 20E system followed by Vitek 2 compact syste

The current study aimed to determine the morphometric and meristic characteristics of the North African catfish Clarias gariepinus (Burchell, 1822). Six specimens of C. gariepinus were collected from the Tigris River, in central Iraq. This study is considered the confirmation first record of this species in Iraq, and the second documentation of this exotic fish. The present species is characterized by a very long dorsal fin, a rounded caudal fin and four pairs of barbels.

      The study area is situated in the northern part of the Arabian Plate. The evolution of the Zagros Foreland basin is related to the compressional tectonic system at the beginning of the Tertiary Period.

This study gives an adequate nomenclature for the Oligocene – Early Miocene Sequence is Missan Group. The Buzurgan Oilfield was chosen to represent the stratigraphic column corresponding to that period. These sediments were subdivided into two cycles, where each one ends by a sequence boundary, equivalent to the lowstand siliciclastic residues in the basin center. The first cycle, Paleocene-Oligocene Epoch, was deposited marly limestone with planktonic foraminifera in the basin center during the transgressive and highst

In this paper, membrane-based computing image segmentation, both region-based and edge-based, is proposed for medical images that involve two types of neighborhood relations between pixels. These neighborhood relations—namely, 4-adjacency and 8-adjacency of a membrane computing approach—construct a family of tissue-like P systems for segmenting actual 2D medical images in a constant number of steps; the two types of adjacency were compared using different hardware platforms. The process involves the generation of membrane-based segmentation rules for 2D medical images. The rules are written in the P-Lingua format and appended to the input image for visualization. The findings show that the neighborhood relations between pixels o

Objective: Zerumbone (ZER) is a well-known natural compound that has been reported to have anti-cancer effect. Thus, this study investigated the ZER potential to inhibit Thymidine Phosphorylase (TP) and the ability to trigger Reactive oxygen species (ROS)-mediated cytotoxicity in non-small cell lung cancer, NCI-H460, cell line. Material and Method: The antiangiogenic activity for ZER was evaluated by using the thymidine phosphorylase inhibitory test. Reactive oxygen species (ROS) production was determined via DCFDA dye by using flow cytometry. Result and Discussion: ZER was found to be potent TP inhibitory with the IC50 value of 50.3± 0.31 μg/ml or 230±1.42 µM. NCI-H460 cells upon treatment with ZER produced sign

At present, the ability to promote national economy by adjusting to political, economic, and technological variables is one of the largest challenges faced by organization productivity. This challenge prompts changes in structure and line productivity, given that cash has not been invested. Thus, the management searches for investment opportunities that have achieved the optimum value of the annual increases in total output value of the production line workers in the laboratory. Therefore, the application of dynamic programming model is adopted in this study by addressing the division of investment expenditures to cope with market-dumping policy and to strive non-stop production at work.

Ten isolates of Klebsiella pneumoniae, seven isolates of Pseudomonas aeruginosa and nine isolates of Staphylococcus aureus, were obtained from 100 urine samples collected from Baghdad hospitals. All isolates were identified biochemically and confirmed by using VITEK 2 and were then tested for their susceptibility towards 6 antibiotics and for phenolic extracts of Thymus vulgaris and Cinnamomum cassia. All bacteria were greatly affected by T. vulgaris, especially K. pneumoniae. Viable count was performed, it was noted that the number of bacterial cells reduced from 1×108 CFU to 1.2× 103, 2×105 and 1.8×106CFU of K. pneumoniae, P. aeruginosa and S. aureus respectively. While C. cassiahad a slight effect on them. K. pneumoniae isola

Nanostructured photodetectors have garnered great attention due to their enriched electronic and optical properties. In this work, we aim to fabricate a high-performance CeO2/Si photodetector by growing a CeO2 nanostructure film on a silicon substrate using the pulsed laser deposition (PLD) technique at different laser energy densities. The impact of laser energy density and the number of pulses on the morphological, optical, and electrical properties was studied. Field emission scanning electron microscopy (FESEM) results show that the CeO2 film has a spherical grain morphology with an average grain size ranging from 33 to 54 nm, depending on the laser energy density. The film deposited at various numbers of laser pulses also has spherical

Leishmania parasites are the causative agent of leishmaniasis. Many studies are inspecting chemical drugs, including the use of miltefosine and amphotericin B, but curative values may be limited for these drugs with side effects due to the chemical origin, therefore, investigating less toxic therapies is essential. The aim of this study was to investigate the effectiveness of artemisinin on Iraqi strain of Leishmania tropica, by experimental macrophage ex vivo infection of amastigotes into mouse macrophage cell-line RAW264.7. Different concentrations (100, 200, 300, 400, 500)μM of artemisinin (ART) were screened to examine the susceptibility of L. tropica amastigotes to invade macrophage cell line along three times of follow up (24, 48 and