PhD, NRI, University of Greenwich
outstanding editor 2022 (Archives of Phytopathology and Plant Protection)
Plant virology, Plant Pathology,Biological Control Of Plant Diseases,Phytopathology,Molecular Plant Pathology,Plant Protection, PCR, Integrated Pest Management, Plant Biotechnology, Molecular Characterization, Geminiviridae, Cloning, Primer, Sequencing, Restriction Digestion, Disease Resistance, Essential Oils. Antiviral, Immunoassay Electrophoresis, DNA, Reverse Transcription
فايروس جزيئي/دكتوراه فايروسات نبات متقدم/ماجستير فايروسات نبات/مرحلة رابعة امراض خضر ومحمية/مرحلة رابعة
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The antiviral activity of leaf extracts from Datura stramonium and tomato plants inoculated with TMV, combined with 20% skimmed milk, was investigated. A TMV isolate was confirmed using bioassay, serological, and molecular approaches and subsequently used to inoculate plants. Tomato plants, both pre- and post-inoculated with TMV, were sprayed with leaf extracts from either TMV-free or infected plants, alone or mixed with 20% skimmed milk. Enzyme-linked immunosorbent assay (ELISA) using tobamovirus-specific antibodies and local lesion tests were conducted to assess antiviral activity based on virus concentration and infectivity in treated plants. The experiment followed a completely randomized design (CRD), and the Least Significant
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