The pathogenicity resulting from Staphylococcus aureus infection has remarkable importance as one of the community-associated bacterial infections, due to the virulent ability of these bacteria to produce biofilms. This study was designed to detect biofilm production in clinical isolates from samples of wounds and urinary tract infections. The expression levels of the icaA gene that is responsible of slime layer production in biofilms was compared in isolates with different biofilm producing capabilities. Fifty seven samples that included 32 samples from urine and 25 samples from wounds were collected from Alwasti Hospital, Al-Kindi Teaching Hospital, and Alzahraa Clinic, Baghdad, Iraq. The bacteria was identified accor

The opportunistic multidrug resistance pathogen Pseudomonas aeruginosa has one or several flagella, and the numbers of these sophisticated machines are regulated by the flagellar regulator gene FleN. The flagellar hook gene FlgE is important for its synthesis, motility and tolerance to antibiotics. Bacteriahave resistance to antibiotics, especially to cephalosporin beta-lactam antibiotics. For the current study, 102 clinical specimens were collected and identified using routine laboratory tests and confirmed by Vitek-2 compact system.  A total of 33 isolates of P. aeruginosa were identified. The antibiotic susceptibility test was done by the Vitek 2 Compact system. Flagellar gene detected by conventional PCR revealed that the FleN

The pathogen "Pseudomonas aeruginosa" is extremely hazardous for people with weak immune systems because it has several virulence factors and can resist antibiotics. During the time period between October 2022 and February 2023, 102 specimens from different clinical sources, including burn, mid-stream urine, wound, ear, and sputum were collected. From 102 clinical specimens, only 33 isolates (32.35%) of P. aeruginosa were identified by Phenotypic characteristics, a biochemical test, the Vitek-2 compact system, and confirmed by Conventional Polymerase Chain Reaction (PCR). The antibiotic susceptibility test AST was done by the Vitek 2-Compact system. The highest resistance percentages were for Cephalosporin category at 8