I’m an assistant lecturer at the University of Baghdad, majoring in microbiology. My main interests are in molecular biology and bioinformatics, especially how we can use these tools to better understand microbial genetics and resistance mechanisms. I enjoy working at the intersection of traditional microbiology and modern molecular techniques, and I’m passionate about using research to support public health and scientific progress.
The spread of antibiotic resistant bacteria is a worldwide problem. Due to the importance of P. aeruginosa as a multidrug resistant bacterium, this study aimed, through molecular techniques, to detect point mutations in chromosomal genes responsible for the quinolones class of antibiotics resistance. A total of 52 isolates from burn infections were identified using specific primers for P. aeruginosa 16S rDNA. Ciprofloxacin minimum inhibitory concentrations (MIC) were estimated using the agar dilution assay. DNA sequences of the quinolone resistance-determining regions of gyrA and parC were determined for detecting the mutations found in these genes and the relations among the i
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