The trichomes and chemical composition of three species of the genus Salvia wild-grown (Salvia lanigera, Salvia spinosa) and cultured (Salvia officinalis) were studied in the Anbar governate, the chemical components of the stem and leaves were studied by Gas chromatography–mass spectrometry(GC-MS), in addition to studying the trichomes of the epidermis in the stem and leaves (upper and lower epidermis) by Light microscope. Important differences appeared to us in the chemical study, where it was found that some compounds were found in species without others, which gives them taxonomic importance, also, the trichomes were important in distinguishing the studied species, the species S. spinosa was distinguished by the presence of glandular trichome with funnel form head on the lower epidermis of the leaves and absence non-glandular multicellular-uniseriate flagellated trichomes. S. officinalis was distinguished by the density of non-glandular multicellular-uniseriate –flagellated trichomes, while S. lanigera was distinguished by non-glandular multicellular-uniseriate –branched trichomes, addition to the presence non-glandular multicellular-uniseriate – straight, curved and glandular trichome with globular head in all studied species.