Photocatalytic materials are being investigated as effective bactericides due to their superior ability to inactivate a broad range of dangerous microbes. In this study, the following two types of bacteria were employed for bactericidal purposes: Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus). The shape, crystal structure, element percentage, and optical properties of Ag9(SiO4)2NO3 were examined after it was successfully synthesized by a standard mixing and grinding processing route. Bactericidal efficiency was recorded at 100% by the following two types of light sources: solar and simulated light, with initial photocatalyst concentration of 2 µg/mL, and 97% and 95% of bactericidal activity in ultra-low photocatalyst concentration of 0.2 µg/mL by solar and simulated light, respectively, after 10 min. The survival rate was studied for 6 min, resulting in 99.8% inhibition at the photocatalyst dose of 2 µg/mL. The mechanism of bactericidal efficiency was found to be that the photocatalyst has high oxidation potential in the valence band. Consequently, holes play a significant part in bactericidal efficiency.