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Synergy of antibacterial and antioxidant activities from crude extracts and peptides of selected plant mixture
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Abstract<sec><title>Background

A plant mixture containing indigenous Australian plants was examined for synergistic antimicrobial activity using selected test microorganisms. This study aims to investigate antibacterial activities, antioxidant potential and the content of phenolic compounds in aqueous, ethanolic and peptide extracts of plant mixture.

Methods

Well diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays were used to test antibacterial activity against four pathogenic bacteria namelyStaphylococcus aureus, Escherichia coli, Bacillus cereus,andPseudomonas aeruginosa. DPPH (2, 2-diphenyl-1- picrylhydrazyl) and superoxide dismutase (SOD) assays were used to evaluate antioxidant activity. HPLC and gel filtration were used for purification of the peptides. Scanning electron microscope was applied to investigate the mode of attachment of the peptides on target microbial membranes.

Results

Aqueous extraction of the mixture showed no inhibition zones against all the test bacteria. Mean diameter of inhibition zones for ethanol extraction of this mixture attained 8.33 mm, 7.33 mm, and 6.33 mm againstS. aureusat corresponding concentrations of 500, 250 and 125 mg/ml whileE .colishowed inhibition zones of 9.33 mm, 8.00 mm and 6.66 mm at the same concentrations.B. cereusexhibited inhibition zones of 11.33 mm, 10.33 mm and 10.00 mm at concentrations of 500, 250 and 125 mg/ml respectively. The peptide extract demonstrated antibacterial activity againstS. aureus,E. coliandB. cereus. The MIC and MBC values for ethanol extracts were determined at 125 mg/ml concentration againstS. aureusandE. coliandB. cereusvalue was 31.5 mg/ml. MIC and MBC values showed that the peptide extract was significantly effective at low concentration of the Australian plant mixture (APM). Phenolic compounds were detected in hot aqueous and ethanolic extracts of the plant mixture. Hot aqueous, ethanol and peptides extracts also exhibited antioxidant activities.

Conclusions

It was concluded that APM possessed good antibacterial and antioxidant activities following extraction with different solvents. The results suggest that APM provide a new source with antibacterial agents and antioxidant activity for nutraceutical or medical applications.

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Publication Date
Sun Jan 27 2019
Journal Name
Journal Of The College Of Education For Women
Effect of the activity of Boswellia Carterii extracts on preservation of ground meat
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Publication Date
Mon Mar 08 2021
Journal Name
Baghdad Science Journal
Impact germination in the content of the chemical components of Homs and sensory qualities of Biskt plant it,
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Publication Date
Sat Oct 04 2025
Journal Name
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Evaluation of antibacterial action of photosensitizer solution activated by diode lamp and three intracanal medicaments (in vitro study)
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Publication Date
Fri Jun 02 2023
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Review Study in Discourse Analysis and Appraisal Theory in Selected Prison Letters by Antonio Gramsci and other Scholars.
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Sat Dec 01 2018
Journal Name
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Compare Estimate Methods of Parameter to Scheffʼe Mixture Model By Using Generalized Inverse and The Stepwise Regression procedure for Treatment Multicollinearity Problem
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Publication Date
Tue Dec 05 2023
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Cytotoxic Activities, Determining Toxin, and Molecular Docking of Ovary Pufferfish (Tetraodon leiurus) in Singkarak Lake as Cancer Chemoprevention Candidate
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Publication Date
Sun Feb 03 2019
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The analysis of the energy spectra of the soil samples show that
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289.62 Bq/kg for U-238, Th-232, k-40 respectively. The radiation
hazard parameters of the natural radionuclides; radium equivalent
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Publication Date
Sat Oct 04 2025
Journal Name
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Wed Dec 14 2016
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Tue Jan 01 2019
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