Background: Titanium implant is widely used in dentistry because of its extraordinary biocompatibility and mechanical properties. To increase bone–implant connection and provide early loading after placement, implant is stored in different storage medium and treated with UV light. Both of them are applicable methods to increase the bioactivity of titanium and overcome the biological aging. This study was designed to assess the effect of vacuum storage method and air storage with and without UV light treated of Cp Ti implant mechanically and histologically. Materials and methods: Titanium screws were acid etched and prepared in four different modes using different storage methods (air or vacuum and, with or without UV treatment. The implant screws were stored 4 weeks and then implanted into rabbit's tibia for histological and torque removal examinations. Results: There was a significant increase in mean value of torque removal test for screws air treated with UV light, vacuum and vacuum treated with UV light (14.5 ,13.8 ,17.92 N.cm respectively after 2 weeks, and (21.5333, 22.5 ,26.47 N.cm respectively after 4 weeks) compared with air storage screws (8.4 N.cm after 2 weeks, and 11.4 N.cm after 4 weeks). Histological examination showed bone formation mineralization and maturation increased with advancing time for air treated with UV light, vacuum and vacuum treated with UV light screws. Conclusion: vacuum storage method and air storage with UV light treated methods of Cp Ti implant had great effect in increasing the osseointegration than air storage methods, while the vacuum storage and treated with UV light have synergistic effects on the biological properties of aged titanium surfaces.