This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.
Generally, there are many problems that people run into; unemployment is one of these common diseases which usually hits the whole communities leaving crucial effects (economic, social, and psychological) that are difficult to handle them. The current study aims to identify the level of emotional disorders among Iraqi unemployed alumni for the academic years (2013-2016). To this end, the researchers have prepared a questionnaire to measure (anxiety, depression, and anger): these criteria distributed over (30) item. As for sample, it limited to (50) male and female student. The findings of study have revealed that the whole sample showed a high level of emotional disorders, and anger disorder showed higher level than the level of (depress
... Show Moreسارت اليهودية على نهج التوحيد بعدما بعث الله تعالى موسى (عليه السلام) ليبلغهم رسالات ربهم، ليكونوا على هدى وصراط قويم، غير أن القوم لم يستقيموا على ذلك الصراط" فبدلوا وأولوا وتمردوا على موسى (عليه السلام) وصوروا الرب حسب أهواء أنفسهم لأنهم جبلوا على حب المادة والإيمان بالمشهود، فصوروا الرب بصور عدة بما يتماشى مع ما الفت عليهم نفوسهم .
ملخـــص البحــــث
يرجع تأريخ ظهور الفساد إلى بداية الخلق والخليقة، إذ اهتم الأديان السماوية بها كثيراً; وذلك لتوعية الناس إلى أن جَلل المصاب تكمن وراء هذه الظاهرة.
والقرآن الكريم اهتم بهذا الجانب اهتماماً بالغاً، فقد ذكر تأثيرات الفساد على المجتمعات عموماً، وعلى الأفراد خصوصاً، ولم تأتِ آية كريمة تتحدث عن آثا
... Show Moreالحمد لله رب العالمين والصلاة والسلام على نبينا محمد صلى الله عليه وآله وصحبه وسلم اجميعن.
وبعد...
فان من أعظم مظاهر اعجازه البياني ذلك التشابه العجيب بين كثير من آياته ، وقد اكتفيت في هذا البحث عن موضوعين ، وقد بينت التشابه بوصف اللفظي حتى لا ينصرف الذهن الى المتشابه المقابل للمحكم ، وقد اظهر البحث سمة الترتيب ، ولاسيما الترتيب داخل الجملة ، ويتضح ذلك في موضوع التقديم والتأخير بين الجمل في ا
... Show Moreالأسطورة ذلك العالم الغريب المجهول الذي يرويه الناس بعضهم يؤمنون به وآخر يبقى بعيد الصلة عنه ولكن لا يمكن أن يشكك به وبما يعتقد به البعض الآخر، تبقى متوارثة من جيل لجيل حتى تتذكر جزئياً أو كلياً وحسب ثقافة الكائن البشري. وفي معطيات كثيرة نجد الإنسان لا يتوانى أن يوعز تلك الأشياء إلى مجهول مسيطر ومهيمن، يستطيع أن يكون متحرراً من كثير من الالتزامات والإشكالات التي يعاني منها الإنسان، وهو في هذا إنما ينتمي أو ي
... Show MoreTo develop a new basis to reduce the phenomenon of stress, one researcher used relaxation techniques is the use of medicinal herbs sedative which is emphasized by some specialists and researchers because of its direct impact on some of the functions of the body as members have a significant impact on the player from the mental and physical Came the importance of research in the use of medicinal herbs sedative and in particular (Lafracin Black, Plantago Sinani great, Alternen) within the program to soothe the psychological players badminton to get rid of the tension felt by sports by athletic competition through the use of a program to ease the psychological with a range of herbs and both means of calming influence of the nervous system. The
... Show MoreToxic dyes are commonly discharged into waste waters and dyes are extensively used in the textile industry so it is necessary to find out efficient and eco-friendly method for treating waste waters resulting from industrial effluences. To achieve this aim the fungus Trichoderma sp. is employed into two lines: first line was self – immobilized fungal pellets in (Czapek – Dox medium) to adsorbs two dyes crystal violet, congo red by concentrations 0.01, 0.02, 0.03, 0.04, 0.05, 0.06 mg/L to both dyes, PH 2, room temperature with shaker in ( hrs.2,hrs.4,hrs.24) , by Uv- Visible spectrum . the removal efficiency of 0.05 mg/L crystal violet by Trichoderma sp was 96%. but there was no remova
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Melanoidins can be diagnosed using the Fourier transform infrared (FTIR) technique. UV/Vis is an effective tool for both qualitative and quantitative analysis of chemical components in melanoidin polymers. The structural and vibrational features of melanoidin synthesized from D-glucose and D-fructose are identical, according to FTIR spectra, with the only difference being the intensity of bands. Using FTIR spectra, the skeleton of melanoidin is divided into seven major regions. The existence of the C=C, C=N, and C=O groups in all melanoidins formed from fructose and glucose with ammonia is confirmed by the areas ranging from 1600 to 1690 cm-1, and the band is largely evident as a broad shoulder. Both melan
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Aleppo bentonite was investigated to remove ciprofloxacin hydrochloride from aqueous solution. Batch adsorption experiments were conducted to study the several factors affecting the removal process, including contact time, pH of solution, bentonite dosage, ion strength, and temperature. The optimum contact time, pH of solution and bentonite dosage were determined to be 60 minutes, 6 and 0.15 g/50 ml, respectively. The bentonite efficiency in removing CIP decreased from 89.9% to 53.21% with increasing Ionic strength from 0 to 500mM, and it increased from 89% to 96.9% when the temperature increased from 298 to 318 K. Kinetic studies showed that the pseudo second-order model was the best in describing the adsorption sys
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