This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.
Basket ball game is one of the collective game that practiced widely with continuous developing .Through it has four chase .it required from it’s the player to have number of physical capable. One of these is endurance of strength in order to perform defense and attack duties. The problem is declining the level of this capable that hands inverting to defence and attack performance. The aim of research is to know the effect of using various exercises in developing endurance of strength and effect in developing two skills. dragging of defense and movement of defender player. The two researchers proposed that there are differences that have statistic function between post-test and pre-test for two experimental and control groups. The experim
... Show Moreهدف البحث إلى بناء مقياس التفكير المنتج بالجمناستك لطالبات، وأعتمد المنهج الوصفي بأسلوب العلاقات الارتباطية على عينة من طالبات المرحلة الثانية في كلية التربية البدنية وعلوم الرياضة للبنات/جامعة بغداد للعام الدراسي (2023/2024) البالغ عددهن الكلي (38) طالبة، وتم اختيار العينة بطريقة عمدياً بأسلوب الحصر الشامل بنسبة (100%) من المجتمع الأصل، واختير منهن (5) طالبات عشوائياً للعينة الاستطلاعية، وتم معالجة النتائج أل
... Show Moreوهو بحث تجريبي أجري على عينة من اللاعبين فئة الناشئين لكرة اليد وبواقع (20) لاعب إذ تم إعداد برنامج متضمن للتمرينات الخاصة بالهجوم الفردي بهدف تطوير بعض القدرات البدنية واللياقة القلبية للاعبين . وتكمن أهمية البحث في استخدام تمرينات الهجوم الفردي، فمن أجل الارتقاء بالمستوى البدني والوظيفي للاعبين من خلال رفع مستوى الأداء البدني لبعض المهارات في لعبة كرة اليد فضلاً عن رفع مستوى اللياقة القلبية وم
... Show Moreإننا نتحدث عن عناصر السرعة مهمة وهي السرعة الانتقالية للأطراف السفلى والسرعة الحركية للذراع المسلحة والسرعة الحركية للطعن وسرعة رد الفعل وسرعة الاستجابة في أداء واحد او ما يسمى بالسرعة التفاعلية؛ لذا هدفت الدراسة الى اعداد تدريبات خاصة تتميز بتمازج أنواع السرعة التي تحتاجها رياضة المبارزة في أداء واحد ومن ثم معرفة تأثير تلك التدريبات في تطوير بعض الجمل الحركية في سلاح الشيش وقد استعمل المنهج التجريبي
... Show MoreThe article presents the results of studies of the species composition of phytopathogens of winter soft wheat grain affected by ear fusarium. For the first time, studies were conducted using a complex of electron microscopic, molecular genetics, and mycological methods. The species F. graminearum dominated in all samples (frequency of occurrence 42.8%). The species F. avenaceum (23.3%), F. sporotrichioides and F. equiseti (8.8% each) were noted with high frequency. Next in descending order of frequency of occurrence: F. oxysporum, F. boothii, F. poae, F. proliferatum, F. accumatum, F. incarnatum, F. vorosii, F. tricinctum, F. cerealis, F. sambuticum. For the first time, 7 species were found for the plain zone of the Pre-Caucasus: F.
... Show MoreEighteen new cyclic imides (maleimides) conncted to benzothiazole moiety through sulfonamide group were synthesized via multistep synthesis.The first step involved preparation of two maleamic acids N-phenylmaleamic acid and N-benzylmaleamic acid via reaction of maleic anhydride with aniline or benzyl amine.Dehydration of the prepared amic acids by treatment with acetic anhydride and anhydrous sodium acetate in the second step afforded N-phenylmaleimide and N- benzyl maleimide which in turn were treated with chlorosulfonic acid in the third step to afford 4-(N-maleimidyl) phenyl sulfonyl chloride and 4-(N-maleimidyl) benzyl sulfonyl chloride respectively.In the Fourth step of this work each one of the two prepared maleimidyl sulfonyl chlorid
... Show MoreBackground: Oral diseases persist to be a major health problem all over the world. Various bacteria and fungi are found to be the possible pathogensresponsible for the oral diseases. Moringa oleifera it is an extraordinary nutritious vegetable tree with many different uses. These leaves have high medicinal value. In the present study, antibacterial and antifungal activities of aqueous extracts of plant Moringa oleifera in comparison to chlorohexidene gluconate and deionized water were determined. Materials and methods: The leaves of plant of Moringa oleifera were collected from College of Pharmacy; Baghdad, Iraq. Tested microorganism (bacterial and fungal) was isolated from different clinical specimens. In-vitroantimicrobial activity was pe
... Show MoreThe education sector suffers from many problems, including the scarcity of schools that can absorb the increasing number of students in light of the increasing population growth rate, as some regions suffer from a lack of opening of new schools or the expansion of existing schools to increase their capacity so that attention is required. The research sought to identify the level of maturity of project management at the research site (Building Department in Al-Karkh I/ Ministry of Education) Being responsible for educational projects and their implementation and to know that, the ten areas of the knowledge guide to project management PMBOK have been adopted according to the PM3 model (one of the models of maturity
... Show MoreObjectives: This study aimed to evaluate the therapeutic potential effects of ascorbic acid or and pyridoxine on diabetic renal microalbumiuria. Methods: This was a cross-sectional study on patients with diabetes mellitus at Al-Yarmouk teaching hospital from January to December 2012, Iraq-Baghdad. Twenty one patients with diabetes mellitus (D.M), 8 IDDM and 13 IDDM were selected from, the duration of disease were ranged from 2-12 years for both type (10 females and 11males) and all enrolled patients ages were ranged from 28-65years. The concentration of total protein in urine was calculated by a biuret colorimetric assay and the urine creatinine level was measured by a modified Jaffe test. Statistical analysis: results are expressed as mean
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