This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.
In today's world the role of leadership in the control factors of the organization different and advancement, and that the possession of the human ability to the development and creation and innovation varies from one individual to another depending on experience; and levels of performance and intellectual; and his mental and physical and other such factors can create a kind of differentiation between the built humans, and this distinction creates our leaders are able to influence others and have the wisdom and connect active individuals and organizations. as the need for organizations of all sizes and levels of leadership transformation is an urgent need to link the application of the various plans and strategies device which direction
... Show More:الملخص/كلية التربية هدف البحث الكشف عن التدريس الفاعل لأساتذة الكيمياء( . /ابن الهيثم، من وجهة نظر الطلبة وتكونت عينة البحث من للعلوم الصرفة80)طالبا( من طلبة قسم الكيمياء وللصفوف الأربعة في القسم، وتم تقويم50)أستاذا جامعيامن أساتذة القسم، واستخدم المنهج الوصفي في تحليل بيانات هذا البحث، وكذلك(علي الأزيرجاوي، استخدم مقياس التدريس الفاعل الذي أعده2010)بعد التحقق منصدقه وثباته، وقد أظهرت نتائج البحث أن متوسط درجا
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The research deals with the collection of Allamah Ali al-Nuri’s guidance on readings, which he included in his book (Ghaith al-Naf’ fi al-Qira’at al-Sabe) and singled out it in a separate study,
إنها وصف واقعي أو خيالي لأفعال تهدف إلى ترفيه أو تثقيف المستمع أو القارئ. (روبرت لويس ستيفنسون) يقول أحد رواد سرد القصص البارزين: هناك ثلاث طرق فقط لكتابة قصة: إما أن يختار الكاتب حبكة ثم يجعل الشخصيات تتناسب معها، أو أن يختار شخصية ويختار الأحداث والمواقف التي تُطور الحبكة. الحبكة هي التصميم أو المنطق أو البنية لنوع معين من الخطاب الذي يُطور وحداته من خلال تسلسل زمني. وهي تُعطي القصة...
