This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.
ملخـــص البحــــث
إن البحث الموسوم (رؤية العلماء اليقينية من مصير السماوات والأرض قبل أحداث الساعة) تناول موضوع خلق السموات والأرض، ومصيرهما قبل أحداث النهاية، من خلال آيات القرآن الكريم والسنة النبوية الشريفة، وبيان الحقائق الثابتة حول تغيير الأرض وطي السموات كطي السجل للكتب، كما بدأه الله تعالى أول خلق، كمادة واحدة، فقال تعالى:
... Show More
هدفت الدراسة التعرف على واقع الريادة الاستراتيجية لإدارة بطولات العاب المضرب في العراق من وجهة نظر القائمين عليها ، وإنتهجت الباحثتان أسلوب دراسة الحالة من منهج البحث الوصفي على عينة من الأفراد العاملين في الإتحادات المركزية والفرعية القائمين على إدارة بطولات ألعاب المضرب في (14) محافظة عراقية عدا محافظات أقليم كردستان يمثلون الهيئات الإدارية للاتحادت المركزية والفرعية فضلا عن الحكام والمدربين ، في ثل
... Show More
The sport took the rapid progress in recent years, as a result of thedevelopment in the principles and theories of sports training, and lambdabenefit sports coaches of the various technical and scientific progress invarious big science. Corrigendum to the skill of jumping is one of thebasic skills and vitality of the basketball player , and on this basis, thebasketball coaches to be bound by the education and training anddevelopment of abilities and physical abilities to their students andathletes to perform this skill, so as to create a team of offensive nature tomake the most effective number of points, often to compensate for theanomalies in the defense, if any.مجلة الریاضة المعاصرة ..... المجلد الثامن ....
... Show Moreملخـــص البحــــث
فإنَّ الاشتغالَ بعلومِ الشريعةِ مِن أشرفِ العلوم في الدنيا منزلةً، وأعلاها عندَ اللهِ تعالى درجةً، وإن خدمةَ هذه العلومِ ومصنَّفاتها فرض على أبناءِ الأُمةِ الإسلامية، دراسةً وشرحاً وتحقيقاً ولاسيما التراثَ المخطوطَ منهُ.
وقد وَقعَ اختيارنا مِن بينِ هذا التُّراثِ الغالي على تحقيق كتابِ "زبدة الفتاوى" للإمامِ محمد بن يوسف القرماني، وتقدَّمتُ به
... Show More
(17)
(6)
Reishi Mushroom, Ganoderma, is considered one of important wood-decaying medicinal mushrooms. This study aimed to identify three samples of this genus in Mosul city in February and April 2019. Three species of Ganoderma were collected from three various trees including Eucalyptus, Morus, and Olea (olive) in Mosul City, Northern Iraq. Their identifications and their DNA sequences were genetically identified by using PCR techniques according to detect nuclear ribosomal internal transcribed spacer (ITS) regions. Results exhibited the finding of Ganoderma resinaceum, Ganoderma applanatum, and Ganoderma sp. This study is first attempt to identify Reishi Mushroom by molecular methods in Iraq. Thus, the current study is considered new good d
... Show More
(2)
The present study introduces description of new species of leafhopper Cicadella latreilla nov. External morphological characters particularly female genitalia were discussed and illustrated, shape of male abdominal apodeme, chaetotaxy of genital style and genital plate of male genitalia, shape of Aedeagus and connective . The external morphological characters were: spotting patterns of vertex, face, pronotum of prothorax, and metothorax, and of lower and upper valves of ovipositors,shape of female seventh abdominasternum.