This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.
يتناول هذا البحث التحول الجذري في الفن الحديث من الكيان المادي (كاللوحة) إلى "الفكرة" كمحور للعمل الفني، مركزًا على الفن المفاهيمي وإشكالية "اللامُقتنى". تم في المبحث الأول تحليل العلاقة المعقدة بين الفن والسوق، وكيف سعى الفن المفاهيمي إلى تفكيك منطق التسلّع من خلال أعمال يصعب اقتناؤها، مثل عمل "الموزة" لكاتيلان، الذي أثار جدلاً حول جدوى التسعير في الفن. أما المبحث الثاني فقد ركّز على فن التركيب كوسيط فني ي
... Show Moreتم تحضرالكاربون المنشط النانوي (NAC) من مخلفات النباتات باستخدام فرن الميكروويف وذلك بخلط نسب مختلفة من قشور الرز مع مخلفات البولي إثلين واطىء الكثافة (LDPE) وكاربونات البوتاسيوم كعامل تنشيط كيميائي في عملية الكربنة، تم تنفيذ كل من عمليتي الكربنة والتنشيط عن طريق تشعيع الموجات الدقيقة بقوة 600 واط لمدة 15 دقيقة، وبنسب وزنية مختلفة لكل من قشور الرز والمخلفات البوليمر. تم قياس المساحة السطحية الخارجية لعينات
... Show Moreفي هذا البحث حضر الكاربون المنشط ذو الحجم الحبيبي في المدى المايكروي والمدى النانوي من قشور الجوز واجريت عملية الكربنة باستخدام فرن المايكرويف ومن ثم استعمل محلول (%50) من حامض الفسفوريك مع المادة المكربنة وبأزمان مختلفة (2,4,8,16,24) ساعة لتحديد الزمن الأفضل لعملية التنشيط في هذا البحث ، وبعد ذلك اضيفت نسب وزنية مختلفة من مخلفات بولي بروبلين عند زمن (8) ساعة وكانت النسب 0.05):,1 0.1:,1 0.15:0.2 ,1: (1:0.25 ,1 للمضاف البوليمري ومس
... Show MoreIn a world of fierce competition companies of different activities strive to strengthen their competitiveness in order to be able to deliver greater value to their customers and gain a distinct sites in competition with other companies in the market at the local and international levels. Every company seeks to focus on one or more of the competitive capabilities in order to turn it into an obvious advantage or a number of competitive advantages to contribute in improving the performance and superiority over its competitors. Therefore, the management of companies no longer need only useful information for the internal aspects of the environment, but also need to include the external environment that includes various and constantly changin
... Show MoreThe present study aims to reveal the extent of the influence of the acquired organizational immune through its dimensions (organizational vaccination, organizational learning, organizational memory, and benchmarking) in the application of knowledge management strategies in its two dimensions (codification strategy, personalization strategy) as well as clarifying that influential relationship between the study variables Because of its importance in reducing resistance to change by responding to the requirements of the environment. A set of main and sub-hypotheses emerged from the study, which was formulated in view of the hypothesis scheme of the study, and i
... Show MoreBidentate Schiff base ligand 3-(3,4-Dihydroxy-phenyl)-2-[(4-dimethylamino-benzylidene)-amino]-2-methyl-propionic acid was prepared and characterized by spectroscopic techniques studies and elemental analysis. The Cd(II), Ni(II), Cu(II), Co(II), Cr(III),and Fe(III) of mixed-ligand complexes were structural explicate through Moler conductance , [FT-IR, UV-Vis & AAS], chloride contents, , and magnetic susceptibility measurements. Octahedral geometries have been suggested for all complexes. The Schiff base and its complexes were tested against various bacterial species, two of {gram(G+) and gram(G-)} were shown weak to good activity against all bacteria.