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STUDY THE INHIBITION ACTIVITY OF PURIFIED BACTERIOCIN FROM LOCAL ISOLATION Lactococcuslactis ssp. lactisagainst SOME PATHOGENIC BACTERIAL SPECIES ISOLATED FROM CLINICAL SAMPLES: دراسة الفعالية التثبيطية للبكتريوسين المنقى من العزلة المحلية Lactococcuslactis ssp. Lactis تجاه بعض البكتريا الممرضة المعزولة من عينات سريرية
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This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.

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Publication Date
Thu Nov 15 2018
Journal Name
Pakistan Journal Of Biological Sciences
Immunomodulation of Zerumbone via Decreasing the Production of Reactive Oxygen Species from Immune Cells
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Scopus (22)
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Publication Date
Fri May 05 2023
Journal Name
مجلة العلوم التربوية والنفسية / مركز البحوث التربوية والنفسية
معوقات وصول المرأة الى المواقع القيادية في الوسط الجامعي من وجهة نظرها
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Publication Date
Mon Dec 01 2025
Journal Name
Al-academy
التحول من اللوحة إلى الفكرة الفن المفاهيمي بين التعبير الذاتي واشكالية اللامقتنى
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يتناول هذا البحث التحول الجذري في الفن الحديث من الكيان المادي (كاللوحة) إلى "الفكرة" كمحور للعمل الفني، مركزًا على الفن المفاهيمي وإشكالية "اللامُقتنى". تم في المبحث الأول تحليل العلاقة المعقدة بين الفن والسوق، وكيف سعى الفن المفاهيمي إلى تفكيك منطق التسلّع من خلال أعمال يصعب اقتناؤها، مثل عمل "الموزة" لكاتيلان، الذي أثار جدلاً حول جدوى التسعير في الفن. أما المبحث الثاني فقد ركّز على فن التركيب كوسيط فني ي

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Publication Date
Tue May 26 2026
Journal Name
Economics And Administrative Studies Journal (easj) (formerly Al-dananeer Journal)
دور الوعي الاستراتيجي في الحد من الحواجز التي تعيق تحقيق الاهداف الاستراتيجية
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Publication Date
Sun Sep 14 2025
Journal Name
Journal Of The College Of Basic Education
تحضير الكاربون المنشط النانوي من مخلفات نباتية ومخلفات بوليمرية بتقنية الاشعة المايكروية
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   تم تحضرالكاربون المنشط النانوي (NAC) من مخلفات النباتات باستخدام فرن الميكروويف وذلك بخلط نسب مختلفة من قشور الرز مع مخلفات البولي إثلين واطىء الكثافة (LDPE) وكاربونات البوتاسيوم كعامل تنشيط كيميائي في عملية الكربنة، تم تنفيذ كل من عمليتي الكربنة والتنشيط عن طريق تشعيع الموجات الدقيقة بقوة 600 واط لمدة 15 دقيقة، وبنسب وزنية مختلفة لكل من قشور الرز والمخلفات البوليمر. تم قياس المساحة السطحية الخارجية لعينات

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Publication Date
Sat Jun 25 2022
Journal Name
Journal Of The College Of Basic Education
تحضير الكاربون المنشط من مخلفات البولي بروبلين وقشور الجوز باستخدام فرن المايكرويف
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في هذا البحث حضر الكاربون المنشط ذو الحجم الحبيبي في المدى المايكروي والمدى النانوي من قشور الجوز واجريت عملية الكربنة باستخدام فرن المايكرويف ومن ثم استعمل محلول (%50) من حامض الفسفوريك مع المادة المكربنة وبأزمان مختلفة (2,4,8,16,24) ساعة لتحديد الزمن الأفضل لعملية التنشيط في هذا البحث ، وبعد ذلك اضيفت نسب وزنية مختلفة من مخلفات بولي بروبلين عند زمن (8) ساعة وكانت النسب 0.05):,1 0.1:,1 0.15:0.2 ,1: (1:0.25 ,1 للمضاف البوليمري ومس

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Publication Date
Wed Jun 19 2019
Journal Name
Journal Of Accounting And Financial Studies ( Jafs )
Accounting information system and its role in enhancing competitivness in the public industrial sector companies in iraq, from the standpoint of the auditors at the federal supeme audit board field study
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In a world of fierce competition companies of different activities strive to strengthen their competitiveness in order to be able to deliver greater value to their customers and gain a distinct sites in competition with other companies in the market at the local and international levels. Every company seeks to focus on one or more of the competitive capabilities in order to turn it into an obvious advantage or a number of competitive advantages to contribute in improving the performance and superiority over its competitors. Therefore, the management of companies no longer need only useful information for the internal aspects of the environment, but also need to include the external environment that includes various and constantly changin

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Publication Date
Fri Jan 01 2021
Journal Name
Journal Of Economics And Administrative Sciences
"Acquired organizational immune and its impact on the application of knowledge management strategies" Analytical study of the opinions of a sample of Lecturer staff at the Technical College of Engineering and the Technical Institute of Amara
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             The present study aims to reveal the extent of the influence of the acquired organizational immune through its dimensions (organizational vaccination, organizational learning, organizational memory, and benchmarking) in the application of knowledge management strategies in its two dimensions (codification strategy, personalization strategy) as well as clarifying that influential relationship between the study variables Because of its importance in reducing resistance to change by responding to the requirements of the environment. A set of main and sub-hypotheses emerged from the study, which was formulated in view of the hypothesis scheme of the study, and i

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Publication Date
Wed May 01 2019
Journal Name
Research Journal Of Chemistry And Environment
Biodiesel from fresh and waste sunflower oil using calcium oxide catalyst synthesized from local limestone
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Scopus (9)
Scopus
Publication Date
Sun Jan 01 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Scienc
Synthesis, Spectral and Bacterial Studies of Mixed Ligand Complexes of Schiff Base Derived from Methyldopa and Anthranilic Acid with Some Metal Ions
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Bidentate Schiff base ligand 3-(3,4-Dihydroxy-phenyl)-2-[(4-dimethylamino-benzylidene)-amino]-2-methyl-propionic acid was prepared and characterized by spectroscopic techniques studies and elemental analysis. The Cd(II), Ni(II), Cu(II), Co(II), Cr(III),and Fe(III) of mixed-ligand complexes were structural explicate through Moler conductance , [FT-IR, UV-Vis & AAS], chloride contents, , and magnetic susceptibility measurements. Octahedral geometries have been suggested for all complexes. The Schiff base and its complexes were tested against various bacterial species, two of {gram(G+) and gram(G-)} were shown weak to good activity against all bacteria.

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