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STUDY THE INHIBITION ACTIVITY OF PURIFIED BACTERIOCIN FROM LOCAL ISOLATION Lactococcuslactis ssp. lactisagainst SOME PATHOGENIC BACTERIAL SPECIES ISOLATED FROM CLINICAL SAMPLES: دراسة الفعالية التثبيطية للبكتريوسين المنقى من العزلة المحلية Lactococcuslactis ssp. Lactis تجاه بعض البكتريا الممرضة المعزولة من عينات سريرية
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This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.

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Publication Date
Wed Jun 01 2011
Journal Name
Journal Of Economics And Administrative Sciences
Diagnosis of the reality of marketing knowledge management"A survey of the views of a sample of telecom operatorsIn the city of Sulaymaniyah "
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This research aimed to diagnose the perception based on Telecommunications of Iraq to the importance of activating knowledge management marketing in possession, as well as Indication of impediments to activate the management of marketing knowledge in the researched companies, also aimed to show the extent of the existence of significant differences in perception based on Telecommunications Iraqi importance of activating the management marketing knowledge in possession. To achieve the objectives of this research, the questionnaire was developed and distributed to a sample of telecommunications companies in the city of Sulaimaniya, was selected on the criterion according to the company's life in terms of seniority in the telecommunication

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Publication Date
Wed Mar 10 2021
Journal Name
Baghdad Science Journal
Morphological study of sex organs and pollen grain of chosen wild species from Solanaceae family in Iraq
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In this study four species from Solanaceae family was conducted. These four species belong to four different genera (Solanum L. ? Physalis L. ?Withania Pauq. ? Lycium L.) The study included morphological characters of sex organs and their pollen grains for these Iraqi wild plants.The results showed that the position of epipetalous stamens , the shape of anther, their dimensions ,and the length of filaments are important taxonomical characters .On the others hand the shape of their ovaries and stigmas are also important characters in distinguish between these four species .Pollen grains are similar in their general shapes and polarities, they have three germinal furrows and germinal apertures, so they are minor in distinguish between these f

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Publication Date
Thu Feb 24 2022
Journal Name
Journal Of Educational And Psychological Researches
The Role of the Faculty of Education in Serving the Community in the Light of the Vision of the Kingdom 2030 from the Faculty Members’ Point of View at the University of Jeddah
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The study aimed at identifying the role of the Faculty of Education in the community service in light of vision of the Kingdom 2030 from the faculty members’ point of view at the University of Jeddah. The study used a descriptive method through a questionnaire administered to a sample of the faculty members.It concluded that the role of the College in serving the community is the development of the right habits in the family and the importance of establishing bridges of cooperation between the Faculty of Education and the surrounding environment and teaching special education teachers how to apply some methods of measurement to determine the needs of students with disabilities. The results also pointed out that there are no statistical

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Publication Date
Sun Jun 30 2019
Journal Name
Journal Of The College Of Education For Women
The Impact of Home Economics on High Performance
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يسعى هذا البحث الى تقديم اطار عملي ونظري حول موضوع "تأثير الاقتصاد المنزلي في تعزيز الاداء العالي" وتم اختبار مخطط الدراسة الفرضي في القطاع التعليمي الحكومي في محافظة البصرة ,ويتضمن عدد من تشكيلات الجامعة التقنية الجنوبية. واستخدمت الاستبانة والمقابلة الشخصية كأسلوب لجمع البيانات للدراسة وكان حجم العينة114 موظف. وقد استخدمت عدد من الاساليب الاحصائية لاختبار فرضيات الدراسة. واظهرت النتائج بأن هناك تأثير ايج

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Publication Date
Tue Mar 01 2005
Journal Name
Albahith Alalami
الجوانب الدعائية في السينما العالمية اثناء النصف الاول من القرن العشرين
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شهدت السينما اثناء عمرها الممتد عبر نحو قرن من الزمن احداثا وتطورات سياسية مهمة، فضلا عن التطورات التقنية والفنية وكانت السينما حاضرة وشاهدة على احداث سياسية بارزة مر بها العالم قبل واثناء وبعد الحربين العالميتين الاولى والثانية وفترة الحرب الباردة وما بعده. وقد حظيت السينما اثناء النصف الاول من القرن العشرين باهتمام كبير من قبل الدول الكبرى والسياسيين فيها فضلا عن الاقبال التجاري للشرك

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Publication Date
Sun Dec 06 2015
Journal Name
مجلة ابن الهيثم للعلوم الصرفة والتطبيقية
فصل وتنقية جزئية لأنزيم الارجنيز من امصال النساء المصابات بتليف الرحم
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Publication Date
Fri Dec 30 2022
Journal Name
Modern Sport
علاقة الثبات والتوازن بدقة مهارة التصويب من القفز عاليا بكرة اليد
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تلخصت مقدمة البحث بأن التحليل  الحركي الميكانيكي وسيلة مهمة بواستطتها يتم تشخيص وتقيم الظاهرة بشكل موضوعي وفق أسس علمية ثابتة ،بأستخدام التقنية الحديثة، وكرة اليد من بين الألعاب التي ظهرت في الآونة الأخيرة بمستوى أداء يجذب إعجاب المشاهد لهذه اللعبة، كماأن الدقة في التصويب تعد مفتاح لتحقيق الهدف من خلال وصول الكرة إلى المكان الصحيح ودخولها إلى المرمى قبلَ أن تكون فرصة للصد . و مشكلة البحث بان اللاعب يهدر

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Publication Date
Tue Jan 01 2002
Journal Name
Al-adab Journal
تقويم برنامج التربية العملية في جامعة بغداد من وجهة نظر الطلبة
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Publication Date
Sat Mar 30 2019
Journal Name
College Of Islamic Sciences
لمحات حقوق الإنسان الفكرية من مقاصد فقه العبادات: (أركان الإسلام مثالاً(
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ملخـــص البحــــث

 

ان الحكمة من تشريع العبادات هي ربط المخلوق بالخالق بصورة دائمة ومستمرة لأجل سعادة هذا المخلوق في الدنيا والاخرة ، فكل عبادة فيها من الغايات والمقاصد التي تحفظ للإنسان فكره وعقله واخلاقه وكرامته وكل ما يملك من حاجات مادية ومعنوية وروحية ، فالعبادات وخاصة اركان الاسلام هي تشريف وتكريم للعباد قبل ان تكون تكليفا ، يسمو الانسان من خلالها فكريا وعقليا وقلبيا

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Publication Date
Sun Jan 01 2023
Journal Name
حوليات أداب عين شمس
تطبيق نظرية العلاقات البلاغية في تحليل اثنتين من السوناتات الرومانسية الإنجليزية
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