This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.
The whole world and the Arab world, especially an important part of this international system, is undergoing a radical transformation at all levels. This mosaic of political, economic, social and military relations and alliances, whether based on the special interests of the major Powers or on the basis of mutual interests, The major transformations to social, economic, political and military conflict and these transformations still bear more surprises, at all levels, nothing remains constant, all changed, relations changed and alliances changed and loyalties fell and the principles of the M changed and the spectacular imperial economies collapsed and the will of the masses was no longer fixed.
ملخّص البحث
يهدف هذا البحث إلى التعريف بنوع من أنواع لطائف الإسناد، وهو الحديث المسلسل بالرواة الشاميين في صحيح البخاري. وأقوال العلماء فيه، ومدى موافقة أقوالهم مع شروط الحديث المسلسل بالرواة. وأهم ما جاء فيه :
- جرى اختيار الرواة الشاميين في صحيح البخاري ، وقد بلغ عدد الأحاديث اثني عشر حديثاً .
- عني شراح حديث صحيح البخاري بذكر لطائف الإسناد ، ولاسيما ابن
إن عمليات نقل وزرع الأعضاء البشرية هي نوع من أنواع الأعمال الطبية، ولذلك فهي تخضع بصفة عامة لذات القواعد التي تحكم الأعمال الطبية، وقد نظمها المشرع عن طريق وضع الضوابط أو الشروط التي تكفل لتلك الممارسات عدم الخروج عن إطارها القانوني المحدد لها بالشكل الذي يضمن المحافظة على الجسم الإنسان واحترام كرامته. وقد أكد المشرع العراقي في قانون عمليات زرع الأعضاء البشرية ومنع الاتجار بها رقم (11) لسنة 2016 على حظر
... Show MoreOf the most prominent elements of the educational process in physical education is a curriculum decision, which is containing a number of elements, including the book Alorteurllmadp study and which must be characterized by a high level of quality is reflected on the nature of the scientific level that will excel by the students after them with knowledge about it and to identify the vocabulary and here emerged the idea and the importance of Find Considering researchers that the quality of the textbook of the article subjects of great importance in the delivery of educational material form the right and perhaps the most prominent subjects and most important for the students of Physical Education is a substance that teaching methods and so to
... Show Moreملخـــص البحــــث
تناول هذا البحث أهم وابرز النظريات الفلسفية المعاصرة وهي نظرية المادية الجدلية ، والتي اذعنت بأفكارها ان المادة هي الاساس الوحيد في اصل الوجود ، وان الموجودات مهما بلغت من مرتبة فهي دون مرتبة المادة ، وعلى هذا الاساس قامت اسسهم ، وكان للعلماء المسلمين موقفا صلبا امام هذه النظرية ، فعملوا على تفنيد هذه الاسس وابطالها بكل الوسائل المتاحة لديهم ، من
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