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Extending the storage life of some fruits by using Pullulan produced from locally isolate Aureobasidium pullulans: Extending the storage life of some fruits by using Pullulan produced from locally isolate Aureobasidium pullulans
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Six isolates of A. pullulans were collected from many sources including Hibiscus sabdariffa (Roselle), old Roofs of houses and bathroom surface that referred as Ap ros1, Ap or2, 3, 4 and Ap bs5, 6 respectively, all these isolates were identified based on morphological characteristics and nutritional physiology profiles, all were able to utilize various carbon and nitrogen sources such as glucose, xylose, sucrose, maltose, ammonium sulfate, ammonium nitrate and ammonium chloride, also they showed positive test for starch and amylase, while α-cellulose, ethanol, and methanol were could not be assimilated and could not grow in 0.05% cycloheximide, the other tests referred to different results among isolates, the Ap ros1 isolate from Hibiscus sabdariffa (Roselle) was selected for production of pullulan.

           Four bacterial strains of two gram (+) and two gram (-) were chosen for the tests. The effect of pullulan coating on bacterial growth was observed in all tested strains, it was 63, 65, 70 and 75% for Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Salmonella typhimuram respectively.

The weight loss of uncoated peach was 3.2, 4.8, 6.1, 7, 7.2, 8.3 and 9.1%, while in coated peach with 10% pullulan were 3.1, 4.5, 5.8, 6.2, 6.8, 7.9 and 8.9%, and in coated peach with 20% pullulan were 2.9, 3.8, 4.1, 4.6, 5.8, 6.4 and 7.6%, when these samples are stored at 3, 6, 9, 12, 15, 18 and 21 days respectively that stored at 25°C, and when uncoated and coated peach with 10 and 20% pullulan stored at 4°C . The weight loss in uncoated peach was 0.01, 0.06, 0.16, 0.23, 0.35, 0.46, 0.6, 0.68, 0.73 and 0.75%, while in coated peach with 10% pullulan were 0.01, 0.05, 0.12, 0.16, 0.2, 0.3, 0.5, 0.58, 0.64 and 0.71%, and in coated peach with 20% pullulan were 0.015, 0.02, 0.04, 0.08, 0.1, 0.12, 0.18, 0.3, 0.46 and 0.5%, when these samples are stored at 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 days respectively.

The weight loss of uncoated pear was 1.4, 2, 2.6, 4.4, 5.5, 6.4 and 7.2%, while in coated peach with 10% pullulan were 1.2, 1.6, 2, 2.4, 4.3, 5.2 and 6.6%, and in coated peach with 20% pullulan were 0.8, 1, 1.4, 1.6, 3, 4.6 and 5.4%, when these samples are stored at 3, 6, 9, 12, 15, 18 and 21 days respectively that stored at 25°C, and when uncoated and coated pear with 10 and 20% pullulan that stored at 4°C. The weight loss is 0.02, 0.07, 0.14, 0.16, 0.3, 0.33, 0.44, 0.45, 0.52 and 0.54%, while in coated peach with 10% pullulan were 0.02, 0.06, 0.11, 0.16, 0.21, 0.3, 0.36, 0.38, 0.44 and 0.46%, and in coated peach with 20% pullulan were 0.01, 0.05, 0.08, 0.09, 0.1, 0.16, 0.19, 0.24, 0.26 and 0.28%, when these samples are stored at 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 days respectively.

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Publication Date
Mon Sep 30 2002
Journal Name
Iraqi Journal Of Chemical And Petroleum Engineering
Removal of Wool Dyes from Industrial Wastewater by Reverse Osmosis Process
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Publication Date
Wed Dec 30 2020
Journal Name
Iraqi Journal Of Chemical And Petroleum Engineering
Removal of Acetaminophen Residues from Wastewater by Bulk Liquid Membrane Process
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   The removal of Anit-Inflammatory drugs, namely; Acetaminophen (ACTP), from wastewater by bulk liquid membrane (BLM) process using Aliquat 336 (QCl) as a carrier was investigated. The effects of several parameters on the extraction efficiency were studied in this research, such as the initial feed phase concentration (10-50) ppm of ACTP, stripping phase (NaCl) concentration (0.3,0.5,0.7 M), temperature (30-50oC), the volume ratio of feed phase to membrane phase (200-400ml/80ml), agitation speed of the feed phase (75-125 rpm), membrane stirring speed (0, 100, 150 rpm), carrier concentration (1, 5, 9 wt%), the pH of feed (2, 4, 6, 8, 10), and solvent type (CCl4 and n-Heptane). The study shows that high ext

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Publication Date
Tue May 23 2023
Journal Name
Journal Of Engineering
REMOVAL OF OIL FROM WASTEWATER BY ADVANCED OXIDATION PROCESS / HOMOGENEOUS PROCESS
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In the present work advanced oxidation process, photo-Fenton (UV/H2O2/Fe+2) system, for the treatment of wastewater contaminated with oil was investigated. The reaction was influenced by the input concentration of hydrogen peroxide H2O2, the initial amount of the iron catalyst Fe+2, pH, temperature and the concentration of oil in the wastewater. The removal efficiency for the system UV/ H2O2/Fe+2 at the optimal conditions and dosage (H2O2 = 400mg/L, Fe+2 = 40mg/L, pH=3, temperature =30o C) for 1000mg/L load was found to be 72%.

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Publication Date
Sat Jul 22 2023
Journal Name
Journal Of Engineering
Removal of Boron from Simulated Iraqi Surface Water by Electrocoagulation Method
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The removal of boron from aqueous solution was carried out by electrocoagulation (EC) using magnesium electrodes as anode and stainless steel electrodes as cathode. Several operating parameters on the removal efficiency of boron were investigated, such as initial pH, current density, initial boron ion concentration, NaCl concentration, spacing between electrodes, electrode material, and presence of carbonate concentration. The optimum removal efficiency of 91. 5 % was achieved at a current density of 3 mA/cm² and  pH = 7 using (Mg/St. St. ) electrodes, within 45 min of operating time. The concentration of NaCl was o. 1 g/l with a 0.5cm spacing between the electrodes. First and second order rate equation were applied to study adsorp

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Publication Date
Wed Sep 30 2020
Journal Name
Iraqi Journal Of Chemical And Petroleum Engineering
Production of 7-methylxanthine from Theobromine by Metabolically Engineered E. coli
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In this work, a novel biocatalytic process for the production of 7-methylxanthines from theobromine, an economic feedstock has been developed. Bench scale production of 7-methlxanthine has been demonstrated. The biocatalytic process used in this work operates at 30 OC and atmospheric pressure, and is environmentally friendly. The biocatalyst was E. coli BL21(DE3) engineered with ndmB/D genes combinations. These modifications enabled specific N7- demethylation of theobromine to 7-methylxanthine. This production process consists of uniform fermentation conditions with a specific metabolically engineered strain, uniform induction of specific enzymes for 7-methylxanthine production, uniform recovery an

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Publication Date
Fri Apr 01 2011
Journal Name
Journal Of Engineering
REMOVAL OF PHOSPHORUS FROM WASTEWATER BY ADSORPTION ONTO NATURAL IRAQI MATERIALS
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This study focused on treating wastewater to remove phosphorus by adsorption onto naturaland local materials. Burned kaolin, porcelinite, bauxite and limestone were selected to be testedas adsorption materials.The adsorption isotherms were evaluated by batch experiments, studyingthe effects of pH, temperature and initial phosphorus concentration. The results showed that at pH6, temperature 20°C and 300 mg/l initial phosphorus concentration; the sorption capacity was0.61, 9, 10 and 13 mg/g at 10 h contact time, for burned kaolin, porcelanite, limestone and bauxiterespectively. As the pH increased from 2 to 10 the removal efficiency for the materials differs inbehaviour. The removal efficiency increased from 40 to 90 % for limestone, and dec

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Publication Date
Sat Mar 01 2014
Journal Name
Al-nahrain Journal Of Science
Adsorption of Ciprofloxacin Hydrochloride from Aqueous Solution by Iraqi Porcelinaite Adsorbent
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Pharmaceuticals are widely distributed in different applications and also released into the environment. Adsorption of Ciprofloxacin HCl (CIPH) on Porcelinaite was studied at ambient conditions. The adsorption isotherms can be well described using the Freundlich and Temkin equations. The pH of the solution influences significantly the adsorption capacity of Porcelinaite, the adsorption of CIPH increased from the initial pH 1.3 and then decreased over the pH rang of 3.8-9. The adsorption is sensitive to the change in ionic Strength, which indicate that electrostatic attraction is a significant mechanism for sorption process. The enthalpy change (∆H) for the adsorption of CIPH onto Porcelinaite signifies an endothermic adsorption. The ∆G

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Publication Date
Thu Jan 01 2015
Journal Name
Journal Of Materials Science And Chemical Engineering
Extraction and Modelling of Oil from Eucalyptus camadulensis by Organic Solvent
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This work was conducted to study the extraction of eucalyptus oil from natural plants (Eucalyptus camadulensis leaves) by organic solvents. the effects of the main operating parameters were studied; type of solvent (n-hexane and ethanol), time to reach equilibrium, the temperature (45°C to 65°C) for n-hexane and (45°C to 75°C) for ethanol, solvent to solid ratio (5:1 to 8:1 (v/w)), agitation speed (0 to 900 rpm) and the particle size (0.5 to 2.5 cm) of fresh leaves to find the best processing conditions for the achieving maximum oil yield. The concentration of eucalyptus oil in solvent was measured by using UV-spectrophotometer. The results (for n-hexane) showed that the agitation speed of 900 rpm, temperature 65°C with solvent to soli

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Publication Date
Wed Mar 01 2017
Journal Name
Journal Of Al-nahrain University Science
Adsorption of Ciprofloxacin Hydrochloride from Aqueous Solution by Iraqi Porcelinaite Adsorbent
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Publication Date
Tue Oct 04 2022
Journal Name
Caspian Journal Of Environmental Sciences
Detecting genetics of several isolated bacterial species from soils by hydrocarbons
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The presence of hydrocarbons in the soil is considered one of the main problems of pollution. In our current study, eight samples isolated from soil saturated with hydrocarbons were taken from different areas of Baghdad, Iraq. In this study, 5 isolates belonging to Pseudomonas aeruginosa by 99%, 4 isolates to Klebsiella pneumoniae by 98%, and 3 isolates to Enterobacter hormaechei by 97% were diagnosed in different ways. A molecular examination was also conducted by 16sRNA. We recorded P. aeruginosa, K. Pneumoniae and E. hormaechei as new local isolates in NCBI. In addition, a comparison was made between our isolates and the global isolates to determine the degree of convergence in the evolutionary line. The genes alkB and nahAc7 were diagno

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