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Extending the storage life of some fruits by using Pullulan produced from locally isolate Aureobasidium pullulans: Extending the storage life of some fruits by using Pullulan produced from locally isolate Aureobasidium pullulans
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Six isolates of A. pullulans were collected from many sources including Hibiscus sabdariffa (Roselle), old Roofs of houses and bathroom surface that referred as Ap ros1, Ap or2, 3, 4 and Ap bs5, 6 respectively, all these isolates were identified based on morphological characteristics and nutritional physiology profiles, all were able to utilize various carbon and nitrogen sources such as glucose, xylose, sucrose, maltose, ammonium sulfate, ammonium nitrate and ammonium chloride, also they showed positive test for starch and amylase, while α-cellulose, ethanol, and methanol were could not be assimilated and could not grow in 0.05% cycloheximide, the other tests referred to different results among isolates, the Ap ros1 isolate from Hibiscus sabdariffa (Roselle) was selected for production of pullulan.

           Four bacterial strains of two gram (+) and two gram (-) were chosen for the tests. The effect of pullulan coating on bacterial growth was observed in all tested strains, it was 63, 65, 70 and 75% for Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Salmonella typhimuram respectively.

The weight loss of uncoated peach was 3.2, 4.8, 6.1, 7, 7.2, 8.3 and 9.1%, while in coated peach with 10% pullulan were 3.1, 4.5, 5.8, 6.2, 6.8, 7.9 and 8.9%, and in coated peach with 20% pullulan were 2.9, 3.8, 4.1, 4.6, 5.8, 6.4 and 7.6%, when these samples are stored at 3, 6, 9, 12, 15, 18 and 21 days respectively that stored at 25°C, and when uncoated and coated peach with 10 and 20% pullulan stored at 4°C . The weight loss in uncoated peach was 0.01, 0.06, 0.16, 0.23, 0.35, 0.46, 0.6, 0.68, 0.73 and 0.75%, while in coated peach with 10% pullulan were 0.01, 0.05, 0.12, 0.16, 0.2, 0.3, 0.5, 0.58, 0.64 and 0.71%, and in coated peach with 20% pullulan were 0.015, 0.02, 0.04, 0.08, 0.1, 0.12, 0.18, 0.3, 0.46 and 0.5%, when these samples are stored at 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 days respectively.

The weight loss of uncoated pear was 1.4, 2, 2.6, 4.4, 5.5, 6.4 and 7.2%, while in coated peach with 10% pullulan were 1.2, 1.6, 2, 2.4, 4.3, 5.2 and 6.6%, and in coated peach with 20% pullulan were 0.8, 1, 1.4, 1.6, 3, 4.6 and 5.4%, when these samples are stored at 3, 6, 9, 12, 15, 18 and 21 days respectively that stored at 25°C, and when uncoated and coated pear with 10 and 20% pullulan that stored at 4°C. The weight loss is 0.02, 0.07, 0.14, 0.16, 0.3, 0.33, 0.44, 0.45, 0.52 and 0.54%, while in coated peach with 10% pullulan were 0.02, 0.06, 0.11, 0.16, 0.21, 0.3, 0.36, 0.38, 0.44 and 0.46%, and in coated peach with 20% pullulan were 0.01, 0.05, 0.08, 0.09, 0.1, 0.16, 0.19, 0.24, 0.26 and 0.28%, when these samples are stored at 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 days respectively.

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Publication Date
Sun Sep 01 2013
Journal Name
Baghdad Science Journal
Purification and Characterization of Endoglucanase from local isolate of Aspergillus flavus
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Endoglucanase produced from Aspergillus flavus was purified by several steps including precipitation with 25 % ammonium sulphate followed by Ion –exchange chromatography, the obtained specific activity was 377.35 U/ mg protein, with a yield of 51.32 % .This step was followed by gel filtration chromatography (Sepharose -6B), when a value of specific activity was 400 U/ mg protein, with a yield of 48 %. Certain properties of this purified enzyme were investigated, the optimum pH of activity was 7 and the pH of its stability was 4.5, while the temperature stability was 40 °C for 60 min. The enzyme retained 100% of its original activity after incubation at 40 °C for 60 min; the optimum temperature for enzyme activity was 40 °C.

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Publication Date
Thu Mar 07 2013
Journal Name
International Journal Of Pharma Sciences
Separation and Purification of Hemolysin from Local Isolate of Serratia marcescens
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Twenty isolates of Serratia marcescens were isolated from inflammation of the urinary tract (UTI)., These isolates were found to produce hemolysin as indicated by blood agar plates in which the hemolysis of red blood cell indicate a positive result. Isolates were selected according to their hemolysis activity by measuring absorbance of hemoglobin at 405 nm that released from red blood cell. Hemolysin was completely purified using 50-75% saturation of ammonium sulphate followed by ion exchange chromatography with DEAE-cellulose then gel filtration chromatography by sepharose 4B. Accordingly molecular weight for the purified toxin was estimated as 45 KD.

Publication Date
Mon Dec 20 2021
Journal Name
Baghdad Science Journal
Isolation and identification of some bacterial isolates from soil contaminated with crude oil and Testing Their Effectiveness
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Bacterial strains were isolated from oil-contaminated soil, in 2018, these isolates were identified, and with the aim of finding out the ability of these isolates to degrede the oil compounds, the color change of medium which added to it isolates was read by the method of Pacto Bushnell Hans. Then the change in the petroleum compounds was read by gas chromatography, for the most effective isolates.

The nine isolated bacterial showed different degrees of color change, and the isolates (Pseudomonas, Bacillus, Micrococcus) outperformed the color change amount (78, 78, 77) %, respectively, compared to the control, and the three isolates together showed the best color change of 90.7. % Compared to the control, and the

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Publication Date
Tue Jan 01 2019
Journal Name
Journal Of Global Pharma Technology
The role of levan produced from pseudomonas fluorescens as antipathogenic substances and phagocytic enhancer against some of bacterial isolates
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Scopus
Publication Date
Wed Jun 29 2016
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
Effect of adding the Flavonoids from Zizypus spina-christi leaves in redox indicators of the fat extracted from Cows, Sheeps bones and storage on defferent temperature and periods.: Effect of adding the Flavonoids from Zizypus spina-christi leaves in redox indicators of the fat extracted from Cows, Sheeps bones and storage on defferent temperature and periods.
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Flavonoids were extracted from Zizyphus spina-christi leaves by Ethyl acetate after acid digested and used as antioxidant. The dried extract was added separately to each sample of fat extracted from hallow cow and sheep bones as follows: T1 cow fat, T2 control for cow fat, T3 sheep fat and T4 control for sheep fat (the control T2 and T4 reffered to samples without added antioxidant).
Samples were stored at -18, 5, 25 and 55 °C for 28 days. The storage trials were conducted at -18, 5 and 25 °C for 28 days for T1, T2, T3 and T4. The chemical indices examined initially and at the end of storage period. PVs was 1.46, 1.46, 1.8 and 1.8 meq/ Kg oil respectively, FFA values were 0.245, 0.245, 0.244 and 0.244% respectively and TBA va

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Publication Date
Sat Jun 01 2019
Journal Name
Journal Of Accounting And Financial Studies ( Jafs )
Measuring the Performance of IT Governance According to COBIT5 Framework by Using the Balanced Scorecard
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The research aims to clarify the COBIT5 framework for IT governance and to develop of a criterion based on Balanced Scorecard that contributes in measuring the performance of IT governance. To achieve these goals, the researchers adopted the deductive approach in the design of balanced scorecard to measure the IT governance at the Bank of Baghdad that was chosen because it relied heavily on IT.

The research has reached a number of conclusions, the most important of which is that the performance of IT department in the Bank of Baghdad falls within the good level that requires constant monitoring, the most committed items of Balanced Scorecard by the Bank were customer, internal operation, growth and finally the financial item; IT

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Publication Date
Sun Mar 07 2010
Journal Name
Baghdad Science Journal
Improvement of thermostable productivity ?-amylase from local isolate Bacillus licheniformis H14.
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(28)Bacterial local isolates of Bacillus sp. were obtained from soil samples. Isolates were tested for thermostable alpha- amylase production on solid media; fifteen isolates were able to develop clear zone around the bacterial growth after floating the plates with iodine reagent (Lugol's solution). There were further tested in submerged culture which led to selection of Bacillus sp. H14since it was the most efficient .Microbial and biochemical tests showed that the local isolate Bacillus sp.H14was refered to the species B.licheniformis that signed as H14 was refered to the species B.licheniformis H14 .,To get ahigher yield of alpha – amylase(48.70unit/mg protein) production from the local isolate B.licheniformis H14 . This study used

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Publication Date
Mon Jan 01 2024
Journal Name
Euphrates Journal Of Agricultural Science
Measuring of Antioxidant enzyme activity and some fruits quality characteristics in apple trees
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In this study, a factorial experiment was conducted using a Randomized Complete Block Design (RCBD) with three replicates to investigate the effects of silicon at four concentrations: 0, 2, 4, and 6 ml/L, designated as S0, S1, S2, and S3, respectively and a calcium-boron combination at three concentrations: 0, (0.5 g/L Ca-EDTA, + 10 mg/L B), and (1 g/L Ca-EDTA, + 20 mg/L B), designated as C0, C1, and C2, respectively. on the activity of antioxidant enzymes and some qualitative traits of fruits. The results indicated that the studied traits were significantly influenced by the factors. Silicon application notably increased enzyme activity, treatment S3 showed the highest activity levels for peroxidase (POD) and superoxide dismutase (SOD) rea

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Publication Date
Sat Dec 31 2022
Journal Name
Al-kindy College Medical Journal
Evaluation of Streptocin SH3, a Bacteriocin produced by Streptococcus sanguinis isolated from Human Dental Plaque
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Background: Bacteriocin is a peptidic toxin has many advantages to bacteria in their ecological niche and has strong antibacterial activity. Objective: The aim of this study was to evaluation of bacteriocin using Streptococcus sanguinis isolated from human dental caries.

Subjects and Methods: Thirty five streptococcus isolates were diagnosed and tested for their production of bacteriocin, and then the optimal conditions for production of bacteriocin were determined.  After that, the purification of bacteriocin was made partially by ammonium sulfate at 95% saturation levels, followed by and gel filtration chromatography

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Publication Date
Mon Sep 30 2019
Journal Name
College Of Islamic Sciences
Some Applied Models of Appreciation   From the Book of Almbsot Imam Muhammad bin Hassan al-Shaibani (d 189 e): - Fundamental study -
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The forensic evidence important for the sources of legislation after the book of God Almighty and the Sunnah of the purified, including what is agreed upon in the protest, which is the book and the Sunnah and consensus, and what is different in the protest, such as measurement and approval and say companions and interests sent, and that the approval of the evidence that did not agree Accordingly, the terms of the fundamentalists differed and their definition differed with the similarity between each other, and the approval in some schools is an argument is not considered as the Shaafa'is, so it may correspond to measurement with them, but the approval at the tap and those who agree is a hidden measurement is likely to clear measurement,I

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