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STUDYING THE EFFECT OF Proteinase K AND α-Amylase ON INHIBITION OF Staphylococcus aureus AND Klebsiella pneumoniae BIOFILM ISOLATE FROM URINARY TRACT INFECTION.: STUDYING THE EFFECT OF Proteinase K AND α-Amylase ON INHIBITION OF Staphylococcus aureus AND Klebsiella pneumoniae BIOFILM ISOLATE FROM URINARY TRACT INFECTION.
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The current study aimed to investigate the viability of biofilm formation klebsilla pneumoniae and Staphylococcus aureus. 440 urine samples were collected from patients suffering from urinary tract infection (UTI) from those who were admitted and visitors to Al-Ramadi Teaching Hospital, Al-Yarmouk Teaching Hospital, Al-Ramadi Teaching Hospital for women and children and , Teaching Laboratories in the Medical City for both genders for a period extended from 5 July, 2017 to 10 October, 2017. Samples were diagnosed by culturing them on a selective media and by biochemical testes , also, diagnosis was ensured by using VITEK-2 compact system. Results showed that K.pneumoniae isolation ratio was 17.1%(68) and S.aureus ratio was 13.1%(52). Their sensitivity was tested against 10 antibiotics, isolates showed uneven resistance according to the type of bacteria. Bacterial biofilm formation viability was detected by qualitative and quantitative methods, isolates per bacteria. Artificial material was used proteinase K and α-Amylase with triple concentrations to each enzyme 33,100,250 µg/ml and 20,100,200 mg/ml respectively, with biofilm inhibition of testing bacteria by precision calibration dishes method. Result showed noticeable decreasing in biofilm formation after enzymes addition in comparison with its formation before enzymes addition, where high decreasing ratio of biofilm according to proteinase K was at concentration 33µg/ml with 95% ratio, while less inhibition to K.pneumoniae, the same concentration showed inhibition viability at 93% and less inhibition viability at 259 µg/ml with 61%. Also, regarding to α-Amylase showed a high inhibition activity at 95%with concentration 100 mg/ml to S.aureus and less inhibition ratio was found at 20 mg/ml with 87%. While to K.pneumoniae , the triple concentrations showed inhibition activity ranged between 36-77%. Statistical analysis showed presence of significant variation between each enzyme concentration to both types of bacteria, also presence of significant variation among different concentration to each bacterial type at (P≤0.01).

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Publication Date
Tue Dec 31 2013
Journal Name
Journal Of The College Of Languages
إيقــاع النفـــس الشـــاعـرة في شعر الشاعر الصّقلي علي بن عبد الرحمن البلـَّنوبي
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درست الباحثة البنية الإيقاعية عند الشاعر الصقلي

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Publication Date
Fri Jan 04 2019
Journal Name
Journal Of Planner And Development
أثر التوجيه بتأثيرأشعة الشمس على التطور العمراني للشوارع التجارية في مدينة بغداد
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Publication Date
Sun Sep 05 2021
Journal Name
جامعة بغداد/ كلية التربية للعلوم الصرفة - ابن الهيثم
امتلاك الطالبات لمهارات التفكير المنظومي المتضمنة في كتاب الرياضيات للصف الثالث المتوسط
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Publication Date
Sat May 23 2026
Journal Name
Alustath Journal For Human And Social Sciences
Musical relational in Andalusian poetry (Ibn al-Abar (d. 595 AH - 658 AH) as an example)
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The voice had a special place in the writting of the Andalusian poet (Ibn al-Abar ) , which aroused my attention because of the sonic capacity of the lettering inside the poetic at Ibn al-Abar poems . So Istudied the qualities of the lettering , structures and their exits . Scientists have not been able to determine the musical from the nonmusical sound , but we find the innate ability of Ibn al-Abar , which was able to determine this by using the lettering the right places and to revival the life to reflect the moments of his life , therefore came votes to express his purposes of praise and pride Etc. The poet Ibn al-Abar could exploit the lettering features in the effect of bilateral – static and moving and its ability to e

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Publication Date
Fri Jan 04 2019
Journal Name
Journal Of Planner And Development
تقييم الأحوال الاقتصادية للمعيشة في مدينة الموصل: السكان والمساكن للمدة 2004-2007
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Publication Date
Sun Oct 01 2017
Journal Name
Journal Of Educational And Psychological Researches
مدى معرفة واستعمال تدريسيو اقسام الجغرافية في كليات التربية لتطبيقات التعليم الالكترونية
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Abstract The present research aims to know the range of Knowledge and the using of teachers of aerography department in colleges of Education to Applicant electronic Learning , So the Sample of the research reached (129) teacher, of the university of Boghdad and Al mustansiria who were chosen by using Randomly sample , and applauding questioner for these perpos that prepared by muhamed bn Ghazi Al Judi – and by using the T-test for one sample and person correlation formula as a statistical means. The research reached to the results which indicate that weakness knowledge and using the theachers of geographe department to the application electronic learning. And according this the researcher put some Recommendation and suggestions. 

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Publication Date
Fri Jan 04 2013
Journal Name
المجلة السياسية والدولية
عوامل تكوين الرأي العام وتأثيره في السياسه الخارجيه المصريه بعد عملية التغيير
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Publication Date
Sun Jul 03 2005
Journal Name
Political Sciences Journal
عرض رسائل واطاريح (اثر التواجد العسكري الامريكي على النظام السياسي في العراق)
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اثر التواجد العسكري الامريكي على النظام السياسي في العراق

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Publication Date
Fri Jun 30 2023
Journal Name
مجلة نسق
اثر استراتيجية القوائم المركزة في تحصيل مادة الكيمياء طلاب الصف الثاني المتوسط
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يهدف البحث التعرف على اثر استراتيجية القوائم المركزة في تحصيل مادة الكيمياء عند طلاب الصف الثاني المتوسط وتم استخدام المنهد التجريبي ذو الضبط الجزئي وكذلك تم اختيار مجامع البحث جميع طلاب المدارس الثانوية والمتوسطة التابعة لمديرية تربية كربلاء

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Publication Date
Sun Jul 03 2005
Journal Name
Political Sciences Journal
ترجمة (عملية صناعة واتخاذ القرار في السياسة الخارجية للرئيس جورج والكر بوش)
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عملية صناعة واتخاذ القرار في السياسة الخارجية للرئيس جورج والكر بوش

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