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STUDYING THE EFFECT OF Proteinase K AND α-Amylase ON INHIBITION OF Staphylococcus aureus AND Klebsiella pneumoniae BIOFILM ISOLATE FROM URINARY TRACT INFECTION.: STUDYING THE EFFECT OF Proteinase K AND α-Amylase ON INHIBITION OF Staphylococcus aureus AND Klebsiella pneumoniae BIOFILM ISOLATE FROM URINARY TRACT INFECTION.
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The current study aimed to investigate the viability of biofilm formation klebsilla pneumoniae and Staphylococcus aureus. 440 urine samples were collected from patients suffering from urinary tract infection (UTI) from those who were admitted and visitors to Al-Ramadi Teaching Hospital, Al-Yarmouk Teaching Hospital, Al-Ramadi Teaching Hospital for women and children and , Teaching Laboratories in the Medical City for both genders for a period extended from 5 July, 2017 to 10 October, 2017. Samples were diagnosed by culturing them on a selective media and by biochemical testes , also, diagnosis was ensured by using VITEK-2 compact system. Results showed that K.pneumoniae isolation ratio was 17.1%(68) and S.aureus ratio was 13.1%(52). Their sensitivity was tested against 10 antibiotics, isolates showed uneven resistance according to the type of bacteria. Bacterial biofilm formation viability was detected by qualitative and quantitative methods, isolates per bacteria. Artificial material was used proteinase K and α-Amylase with triple concentrations to each enzyme 33,100,250 µg/ml and 20,100,200 mg/ml respectively, with biofilm inhibition of testing bacteria by precision calibration dishes method. Result showed noticeable decreasing in biofilm formation after enzymes addition in comparison with its formation before enzymes addition, where high decreasing ratio of biofilm according to proteinase K was at concentration 33µg/ml with 95% ratio, while less inhibition to K.pneumoniae, the same concentration showed inhibition viability at 93% and less inhibition viability at 259 µg/ml with 61%. Also, regarding to α-Amylase showed a high inhibition activity at 95%with concentration 100 mg/ml to S.aureus and less inhibition ratio was found at 20 mg/ml with 87%. While to K.pneumoniae , the triple concentrations showed inhibition activity ranged between 36-77%. Statistical analysis showed presence of significant variation between each enzyme concentration to both types of bacteria, also presence of significant variation among different concentration to each bacterial type at (P≤0.01).

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Publication Date
Mon Jan 01 2018
Journal Name
جامعة بغداد - كلية التربية للعلوم التطبيقية - إبن الهيثم
دليل التجارب العملية في الكيمياء اللأعضوية / المرحلة الثانية
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تعد التجارب المختبرية من أهم الوسائل التي تمكن من فهم خصائص المواد الكيميائية وسلوكها، كما إنها الوسيلة الوحيدة لتحضير المركبات الكيميائية المختلفة. وقد أدى تطور التكنولوجيا في الوقت الحاضر إلى إستخدام أدوات وأجهزة قياس في المختبر مكنت من زيادة دقة نتائج التجارب وسرعة الحصول عليها. أن تفعيل المختبر وإجراء الأنشطة العملية يمكن أن تحقق عدة أمور منها : 1- تُنمي في الطالب مهارات التفكير العلمي ( الملاحظ

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Publication Date
Mon Apr 29 2019
Journal Name
Arab Science Heritage Journal
ادارة إدارة المكتبات في العراق بين التراث والمعاصرة
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Iraqies has a spot in administration of the libraries and organized it since the reign of civilizations , the ancient libraries in the world were in the mesopatemia, then Baghdad had crown to be Islamic civilization when it hold the greatest ones as Bait Al-Hikma according to its organization as well as the persons who were responsilble for libraries must be of higher education and thoutful ones .

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Publication Date
Tue Jan 01 2008
Journal Name
Journal Of Educational And Psychological Researches
مواجهة مشكلة المخدرات في العراق بين الواقع والمستقبل
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لم تكن المخدرات وليدة هذا العصر فقد عرفها الانسان منذ القدم وحاربتها المجتمعات القديمة ثقافياً واجتماعياً ودينياً ، حيث تعتبر المخدرات اخطر كارثة عرفتها البشرية في تاريخها وبدأت تتطور لتصبح تجارة عالمية غير مشروعة ترعاها عصابات منظمةهدفها تدمير طاقات وقدرات وقيم الشباب آمال هذه الامة وكنزها الحقيقي وهذا يعني تفريغ المجتمع من العقول المبدعة فيه حتى يبقى مجتمعنا متخلفاً من السهل السيطرة عليه.

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Publication Date
Sun Jun 18 2023
Journal Name
Arab Science Heritage Journal
الاحوال العامة للمدن الاسلامية في رحلة ابن بطوطة
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        This research shows Ibn Battuta's approach in his book Ibn Battuta's Journey that is called "Tuhfet Al-Anzar fi Ghera'ib Al-Amsar wa Eaja'ib-Al-Asfar", which explore the urban aspects of shops, markets, palaces, houses, mosques, monasteries and other antiquities within its pages, whether in terms of the site or in terms of studying it and the plans that explained the basis for the art and architecture of these cities, the intellectual dimensions in their civilizational history are clarified

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Publication Date
Sat Jun 01 2019
Journal Name
مجلة البحوث والدراسات الإسلامية
اثر الفكر الاصولي اللغوي في بناء المجتمع الاسلامي
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يعد علم أصول الفقه السبيل إلى استنباط الأحكام من النصوص الشرعية، وبقواعده يسدد المجتهد نظره في أحكام الشريعة ويعينه على الوصول إلى الحقيقة. فهو علم دقيق المسلك، يقصده كل من يتحاشى السطحية في فهم النصوص الشرعية وتفسيرها وتكييف الوقائع والتصرفات الشرعية على النصوص تكييفا صحيحا يعصم العقل عن الخطأ، فهو بالنسبة إلى العلوم الشرعية كالنحو بالنسبة إلى العلوم العربية. وقد نال هذا العلم من جهد العلماء الحظ الأكبر وا

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Publication Date
Sat Apr 17 2021
Journal Name
رؤوية افريقيا
المصالح الفرنسية في القارة الافريقية بعد عام 2000
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تنشر الدولة الفرنسية قواتها العسكرية في جميع دول القارة الافريقية بهدف السيطرة عليها وتحقيق مصالحها الشخصية

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Publication Date
Fri Jan 04 2019
Journal Name
Journal Of Planner And Development
مفاهيم وتطبيقات لإمكانية التخطيط والتصميم المستدام في السكن
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Publication Date
Tue Sep 27 2022
Journal Name
Arab Science Heritage Journal
كتب الخطط في مصر حتى نهاية العصر المملوكي
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Books of plans are a type of writing in Islamic history , its a type of work whose authors have strived to serve as a comprehensive historical encyclopedia, and they focused most of their attention on highlighting the various aspects of the history of Egypt

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Publication Date
Wed Jan 02 2019
Journal Name
Journal Of Educational And Psychological Researches
Parents Role in Social Personality Formation for Children
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Experts of Psychology and education emphasized that childhood is one of the most important stages in the formation of human personality, and the most influential in his public life, especially the stage in which he lives within his family, which must provide him with the requirements of healthy growth of physical, emotional, moral and social. Emotional-social connection of the child to his family, is especially important in determining the characteristics of his social character, in accordance with the norms and values ​​prevailing in society. This requires the child to be cared for and cared for, and to deal with him in a healthy social manner. So as to achieve positive growth and harmony in the process of social control in internal

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Publication Date
Tue Dec 11 2018
Journal Name
Arab Science Heritage Journal
غربة غربة الشعراء المشردين وحنينهم في العصر الجاهلي
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Alienation, and Westernization and away, whether forcibly sense exile or voluntary in the sense of migration, it will generate nostalgia, and a sense of overwhelming homesickness spatial or alienation spiritual (emotional) and perhaps alienation resulting from exile or forced deportation by the authority

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