Many of the dynamic processes in different sciences are described by models of differential equations. These models explain the change in the behavior of the studied process over time by linking the behavior of the process under study with its derivatives. These models often contain constant and time-varying parameters that vary according to the nature of the process under study in this We will estimate the constant and time-varying parameters in a sequential method in several stages. In the first stage, the state variables and their derivatives are estimated in the method of penalized splines(p- splines) . In the second stage we use pseudo lest square to estimate constant parameters, For the third stage, the remaining constant parameters and time-varying parameters are estimated by using a semi-parametric regression model and then comparing this method with methods based on numerical discretization methods, which includes two stages. In the first stage we estimate the state variables and their derivatives by (p spline) , In the second stage we use Methods of numerical discretization methods (the Euler discretization method and the trapezoidal discretization method), where the comparison was done using simulations and showed the results superior to the trapezoidal method of numerical differentiation where it gave the best estimations to balance between accuracy in estimation And high arithmetic cost.
