Background: Mycotoxins have a significant impact on population health worldwide. Ochratoxin is a common mycotoxin that can be detected in the serum of healthy people due to its prevalence in food. Researches on ochratoxin and its metabolites in biological fluids can help us better understand the toxin's biological impacts. Objective: The aim of this study was to investigate whether individuals in Baghdad, Iraq, were exposed to ochratoxin A. Methodology: Serum samples were obtained from 90 healthy persons (ages 14–40 years) and evaluated for ochratoxin A using the human Ochratoxin A ELISA Kit, an accurate, quantitative, and sensitive technique (limit of detection 0.34 ng/ml). Results: We detected Ochratoxin A in all serum samples, with a mean concentration of 10.447 ng/ml (ranging from 2.66 to 71.447 ng/ml). The most prevalent concentration of ochratoxin A was between 10.1 and 20 ng/ml. The correlation between OTA concentration level and age groups was significant at the 0.01 level. The study did not show a significant difference in the concentration of ochratoxin between males and females. Conclusion: This study provides useful information about human exposure to OTA in Baghdad. Further research in youngsters, elderly, and adult cohorts from various regions is recommended. © 2025, Egyptian Society for Medical Microbiology (ESMM). All rights reserved.
