Background: Removal of bacteria from the pulp system by instrumentation of an infected root canal, will be significantly reduced the number of bacteria, but it is well documented that instrumentation alone can-not clean and kill all bacteria found on the root canal walls. Antibacterial irrigants are needed to kill the remaining microorganisms. The aims of this study was to assess antibacterial effect of titanium tetrafluoride (TiF4) solution and brewing green tea against root canal bacteria and to compare with sodium hypochlorite and normal saline through microbiological and molecular studies. Materials and methods: Microbiological study was carried out to determine the concentration of titanium tetrafluoride and brewing green tea at which they exert antibacterial effect against ten swabs that had been taken from necrotic root canals that were incubated aerobically and anaerobically by paper disk diffusion test, whilemolecular study carried out among Forty children in which the antibacterial effect of titanium tetrafluoride and brewing green tea compared with sodium hypochlorite and normal saline were assessed by real time polymerase chain reaction using SYBR Green. Results: The microbiological study results showed that TiF4 achieved maximum antibacterial effect at concentration 5% against aerobic and anaerobic bacteria while green tea exhibited antibacterial effect when brewed for 20 minutes at concentration 100mg/1ml against staphylococcus aureus, but not active against other microorganisms like Escherichia coli and streptococcus. While results of molecular study illustrated that sodium hypochlorite remained the most effective endodontic irrigant solutions followed by titanium tetrafluoride then green tea while normal saline showed no antibacterial effect. Statistically titanium tetrafluoride, green tea and sodium hypochlorite have significant differences compared to normal saline. Conclusions: This study revealed that, titanium tetra fluoride and brewing green tea can be used as antibacterial irrigant solutions for root canal treatment in children.
In this research, the electrical characteristics of glow discharge plasma were studied. Glow discharge plasma generated in a home-made DC magnetron sputtering system, and a DC-power supply of high voltage as input to the discharge electrodes were both utilized. The distance between two electrodes is 4cm. The gas used to produce plasma is argon gas which flows inside the chamber at a rate of 40 sccm. The influence of work function for different target materials (gold, copper, and silver), - 5cm in diameter and around 1mm thickness - different working pressures, and different applied voltages on electrical characteristics (discharge current, discharge potential, and Paschen’s curve) were studied. The results showed that the discharge cur
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The Ceramic was a part from the humanitarian production that masseure the development and promotion of the Nations through decades. The Ceramic development involve wide and more techniques. One of these techniques that appear through the developmental centuries, the ( Alkaro Ceramic )technique, this technique depended the simplicity that comitted on religions thoughts through their development and its origin, so it had connected with these religions thoughts and had transmitted to the differnt Nations and they development in it to stay as a connection to the original key although its a way to keep them from adoption a new path. The tracker of the Ceramic fine art through its modernal level could knew and touched the Enormons transformati
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Improving" Jackknife Instrumental Variable Estimation method" using A class of immun algorithm with practical application
KA Hadi, AH Asma’a, IJONS, 2018 - Cited by 1
Methylotrophs bacteria are ubiquitous, and they have the ability to consume single carbon (C1) which makes them biological conversion machines. It is the first study to find facultative methylotrophic bacteria in contaminated soils in Iraq. Conventional PCR was employed to amplify MxaF that encodes methanol dehydrogenase enzyme. DNA templates were extracted from bacteria isolated from five contaminated sites in Basra. The gene specific PCR detected Methylorubrum extorquens as the most dominant species in these environments. The ability of M. extorquens to degrade aliphatic hydrocarbons compound was tested at the laboratory. Within 7 days, gas chromatographic (GC) studies of remaining utilize
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