INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION AND GERMINATION OF DATE PALM CV BARHI BY USING CELL SUSPENSION CULTURE TECHNIQUEث
Publication Date
Thu Jan 12 1995
Journal Name
مجلة العلوم الزراعية العراقية 26 (1), 88-92
Publication Date
Thu Dec 01 2016
Journal Name
Journal Of Cleaner Production
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(44)
Publication Date
Fri Mar 17 2017
Journal Name
Elixir Mech. Engg. 109 (2017) 47879-47881, Www. Elixirpublishers. Com …
Publication Date
Thu Jan 24 2019
Journal Name
Multifaceted Protocol In Biotechnology
Culturing and Maintaining Mammalian Cell Culture
Mammalian cell culture refers to culturing mammalian cells in a medium that provide nutrients for cells to be able to grow in vitro under environment that closely mimic the in vivo conditions. By enabling culturing these cells outside living biological entities, investigation on intra- and intercellular activities and flux; genetic and phenotyping analysis; proteomics, study of toxicology, drug discovery and development can be carried out without manipulation of living animals. In this chapter, detail protocol of media preparation, cell culture maintenance and preservation are elaborated for both types of mammalian cell culture, monolayer or suspension cultures. Determination of number of cells is discussed as well.
Publication Date
Tue Jan 16 2018
Journal Name
Design And Manufacture An Automatic Knife For Date Palm Tree Frond Cutting Operates By Frequency Theory Cutting Mra Abdulrazak A. Jasim
Publication Date
Thu Feb 16 1995
Journal Name
. مجلة العلوم الزراعية العراقية 26 (1), 84-87
Publication Date
Sun Dec 01 2019
Journal Name
Regenerative Therapy
In vitro periodontal ligament cell expansion by co-culture method and formation of multi-layered periodontal ligament-derived cell sheets
Keywords:
Collagen graft
Mesenchymal stem cells
Periostin
Isolation PDL
Scaffold
Three-dimension culture
Objective: Per-implantitis is one of the implant treatment complications. Dentists have failed to restore damaged periodontium by using conventional therapies. Tissue engineering (stem cells, scaffold and growth factors) aims to reconstruct natural tissues. The paper aimed to isolate both periodontal ligament stem cells (PDLSCs) and bone marrow mesenchymal stem cells (BMMSCs) and use them in a co-culture method to create three-layered cell sheets for reconstructing natural periodontal ligament (PDL) tissue. Materials and methods: BMMSCs were isolated from rabbit tibia and femur, and PDLSC culture was established from the lower right incisor. The cells were co-cultured to induce BMMSC differentiation into PDL cells. Cell morphology, stem cel
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Publication Date
Tue Nov 01 2022
Journal Name
Journal Of Cleaner Production
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Publication Date
Tue Oct 01 2013
Journal Name
International Journal Of Biological Macromolecules
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Publication Date
Tue Nov 01 2022
Journal Name
Journal Of Cleaner Production
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