The purpose of this work was to study the effects of the Nd:YAG laser on exposed dentinal
tubules of human extracted teeth using a scanning electron microscope (SEM). Eighty 2.5mm-thick
slices were cut at the cementoenamel junction from 20 extracted human teeth with an electric saw. A
diamond bur was used to remove the cementum layer to expose the dentinal tubules. Each slice was
sectioned into four equal quadrants and the specimens were randomly divided into four groups (A to D ).
Groups B to D were lased for 2 mins using an Nd:YAG laser at 6 pulses per second at energy outputs of
80 , 100 and 120 mJ. Group A served as control. Under SEM observation, nonlased specimens showed
numerous exposed dentinal tubules. SEM observation revealed that application of Nd:YAG laser at
energy output of 80 mJ may cause melting of dentin and partial closure of exposed dentinal tubules
without dentin surface cracking. But when the energy output raised to 100 and 120 mJ more sealing of
dentinal tubules occurs. Heat generation occurred in all lased groups but the amount of temperature
increase was within the permissible limits(less than 5.5⁰C). The measured sealing depths were from 3-
5μm for groups B-D and the width of the created groove was 30μm in all lased groups. In conclusion
lasing with nanosecond Nd:YAG with a wavelength of 1064nm at 120mJ with 6Hz for 2 minutes (group
D) shows the efficient laser parameters for complete sealing of exposed dentinal tubules without
temperature increase to the damaging level of the pulp tissue.
Receipt date:06/23/2020 accepted date:7/15/2020 Publication date:12/31/2021
This work is licensed under a Creative Commons Attribution 4.0 International License
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