This study aimed to determine the effect of green bismuth oxide (BiO) NPs against multidrug-resistant (MDR) Pseudomonas aeruginosa (P. aeruginosa) from wound infections. Among 450 wound samples collected from patients admitted to the hospital, 200 P. aeruginosa isolates were identified. MDR strains of P. aeruginosa were detected by disc diffusion method. BiO NPs were synthesized using wild Bacillus subtilis (B. subtilis) strain and infrared spectroscopy, X-ray diffraction and scanning electron microscopy techniques. The antibacterial effect of the NPs compared to antibiotics against MDR strains was evaluated using a standard disk diffusion method. BiO NPs were synthesized at 0.005 M concentration of solution. According to the SEM images, the BiO NPs were irregular in size and ranged from 23-41 nm. According to the disk diffusion method, the highest inhibitory effect of BiO NPs was observed against MDR P. aeruginosa at 2000 ppm. The MIC and MBC of Bi-O NPs at 2000 ppm and >2000 ppm was observed against 97/200 (47.5%) of MDR P. aeruginosa. Eighty percent and 20% of P. aeruginosa produced strong and moderate-level biofilms, respectively. In the presence of sub-inhibitory concentration (1200 ppm) of Bi-ONPs, 52 (26%) isolates produced strong-level biofilms (p=0.001) and 22 (11%) produced moderate-level biofilm (p=0.029), while 53% of remaining isolates produced weak biofilms. The results exhibited a significant decrease in biofilm formation in the presence of Bi-O NPs. BiO NPs exerted an antibacterial effect that 2000 ppm and had a significant inhibitory effect against P. aeruginosa biofilms
One hundred forty three of Klebsiellapneumoniae isolates had been collected from some hospitals in Baghdad city. The isolates were taken from different clinical specimens.Antimicrobial susceptibility test was carried out towards fifteen antimicrobial agents by using Vitek2 system with Antimicrobial susceptibility test cards. The results of antibiogram showed that the local isolates were possess highly resistance towards most antimicrobial agents under study. The high resistance wastoAmpicillin while the low resistance was to Imipenem.Two methods were used for detection of Extended Spectrum Beta Lactamases (ESBLs) production; first methods by using of Vitek2 system,thesecondmethods by using of polymerase chain reaction (PCR) technique to dis
... Show MoreStaphylococcal enterotoxin B (SEB) is a potent superantigen produced by
Sage (Salvia officinalis), belong to Labiatae family is indigenous to Iraq and other Mediterranean areas but now cultivated world- wide, principally for its use as culinary herb. In the present study preliminary screening for the important phytochemical natural product groups indicated the presence of flavonoid, saponin, hyrolysable and condensed tannin groups. The antibacterial activity of two concentrations 10 mg/ml and 100 mg/ml of chloroform and hydroalcoholic extracts from Salvia officinalis leaves was evaluated against four strains of gram negative bacteria (Escherichia coli, Pseudomonas arigenossa, Klebsiella pneumonia, and Proteus spp) and two str
... Show MoreThe antibacterial activity of some extracts of A. eupatoria (aqueous and ethanolic) against some pathogenic bacteria (Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli ) and their activity on wound healing in rats , also the presence of some active compounds in both extracts were detected . The results showed that the ethanolic extract was more effective on inhibiting tested bacteria than the aqueous extract . P.aeruginosa was the most resistant bacteria, while highest inhibition zone appeared on E.coli (20 mm) .There was a moderate activity against S.aureus with inhibition zone 15 mm. by using ethanolic extract (10 mg/ml) . The phytochemical analysis for detection of active compounds revealed the presence of Carbohydrate
... Show MorePotential pattern of foodborne bacteriophages against multidrug-resistant pathogens was a promising hygienic strategy module. Post-antibiotics era becomes evident due to emerging of dramatic episodes of infectious foci harboring biofilm and multidrug-resistant pathogens transferred mainly throughout food chain. Vancomycin-resistant enterococci (VRE) were struggling among these new infectious emergencies. Phenotypic epigenetic transit tolerant drift cascaded by genetic resistant shift behaviors of recalcitrant VRE forbidden clones recovered from mastitis cases in Cows from territories of Abu-Ghraib, Al- Fudhaliyah and Al-Sadrya in Baghdad ecosystem were combated by redirected lytic bacteriophages cocktails recovered from the same raw-milk ec
... Show MoreIn this research ,Undoped Nio and 1%Li doped Nio thin films were deposited utilizing chemical spray pyrolysis on the glass substrates heated (450C). The effects of non-thermal plasma on the structural and optical properties were studied. XRD measurement shows that Nio and Nio:1%Li films were found to be polycrystalline and have cubic structure with a preferred orientation (111). Decreased crystal size after exposure especially at (7) sec. AFM data indicate that the surface roughness average and (RMS) values of the prepared doped films are increasing after exposure to plasma, the transmittance increases after doped samples exposure to plasma, it was found that the energy gap value decreased when doped samples exposure to plasma, also, thickn
... Show MoreWe have studied the effect of applying an external magnetic field on the characteristics of iron oxide (IO) nanoparticles (NPs) synthesized by pulsed laser ablation in dimethylformamide (DMF). The NPs synthesized with and without applying of magnetic field were characterized by Fourier transformation infrared spectroscopy (FT-IR), UV–Vis absorption, scanning electron microscope (SEM), atomic force microscope (AFM), and X-ray diffraction (XRD). SEM results confirmed that the particle size was decreased after applying magnetic field.