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Gelatin Nanoparticles as a Carrier for Acriflavine and Triethylenetetramine: Cytotoxicity and Antibacterial Activity
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ABSTRACT<p> Despite extensive investigation as biocompatible drug carriers, gelatin nanoparticles (GNPs) have not been thoroughly assessed for carrying chemically distinct cationic molecules such as acriflavine (ACF) and triethylenetetramine (TETA). In this study, we hypothesize that GNPs can effectively encapsulate ACF and TETA, forming stable delivery systems with distinct antibacterial and cytotoxic activities. ACF encapsulated in gelatin was prepared adapting desolvation technique. The procedure involved stirring of an aqueous solution of gelatin and ACF at room temperature, the pH was titrated to eight using NaOH followed by addition of ethanol. The resulting nanoparticles were treated with glutaraldehyde solution for stabilizing of formed nanoparticles and the cross‐linking was achieved under stirring for 24 h. Finally, nanoparticles were decorated with TETA. An optimization study was conducted on TETA‐ACF‐GNPs using FT‐IR in comparison to untreated gelatin and ACF, a new peak associated with the imine (C═N) formed bonds appears at 1643 cm <sup>−1</sup> providing evidence of the successful formation of gelatin‐ACF conjugate. With respect to morphology, surface texture, and roughness, AFM result shows that the particle density of TETA‐ACF‐GNPs increased dramatically with a substantial decrease in particle size and a highly uniform surface topography, which are indicative of successful encapsulation. Higher entrapment efficiency and production yield were proved using UV–visible spectrophotometer. TETA‐ACF‐GNPs exhibit good antibacterial activity against <italic>Staphylococcus aureus</italic> and <italic>Pseudomonas auruginosa</italic> as well as less toxicity against normal human MRC‐5 fetal lung fibroblast cells. Accordingly, gelatin might be useful as protein containers to be combined with ACF and TETA for improving pharmaceutical properties of drugs. </p>
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Publication Date
Fri Jan 01 2010
Journal Name
International Journal Of Communication Networks And Distributed Systems
A novel Radon-based multi-carrier direct sequence code division multiple access transceiver design and simulation
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Multi-carrier direct sequence code division multiple access (MC-DS-CDMA) has emerged recently as a promising candidate for the next generation broadband mobile networks. Multipath fading channels have a severe effect on the performance of wireless communication systems even those systems that exhibit efficient bandwidth, like orthogonal frequency division multiplexing (OFDM) and MC-DS-CDMA; there is always a need for developments in the realisation of these systems as well as efficient channel estimation and equalisation methods to enable these systems to reach their maximum performance. A novel MC-DS-CDMA transceiver based on the Radon-based OFDM, which was recently proposed as a new technique in the realisation of OFDM systems, will be us

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Publication Date
Thu Oct 26 2023
Journal Name
Farmacia
THE DEVELOPMENT OF A BRAIN TARGETED MUCOADHESIVE AMISULPRIDE LOADED NANOSTRUCTURED LIPID CARRIER
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Publication Date
Tue Jun 17 2025
Journal Name
Baghdad Science Journal
Synthesis, Characterization and Studying Mass Spectra and Thermal Analysis for Complexes of Some Metal Ions and Determining their Activity as Antioxidants
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The new azo dye was prepared from the reaction of the diazonium salt derived from 3-aminophenol with 2- hydroxyquinoline, then it was used to prepare a series of complexes with the chlorides of cobalt, chromium, copper, nickel, platinum, palladium and ammonium molybdate. The ligand was identified by a proton and carbon nuclear magnetic resonance spectroscopy, and the compounds were collected. The prepared materials were subjected to infrared, ultraviolet-visible, and mass spectrometry, as well as thermogravimetric analysis, differential calorimetry, and elemental analysis. Conductivity, magnetic susceptibility, metal content, and chlorine content of the complexes were also measured. The results showed that the ligand behaves in a trigonal b

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Publication Date
Tue Jan 01 2019
Journal Name
Biochem. Cell. Arch
BIOSYNTHESIS OF SILVER NANOPARTICLES USING MINTS LEAF EXTRACT AND EVALUATION OF THEIR ANTIMICROBIAL ACTIVITY
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The aim of this study is to synthesize an easy, non-toxic and eco-friendly method. Silver nanoparticles which were synthesized by leaf extract of mint were characterized by UV-Visible Spectroscopy which appears UVVisible spectrum of demonstrated a peak 448 nm corresponding to surface Plasmon resonance of silver nanoparticles, Fourier Transform Infrared Spectroscopy (FTIR); functional groups involved in the silver nanoparticles synthesis were identified, the presence of silver nanoparticles was confirmed by X-ray diffraction (XRD) and Atomic Force Microscope (AFM) analysis clearly illustrated that the shape of silver nanoparticles was spherical and the size of the silver nanoparticles has been measured as 55- 85 nm. Evaluation of its antimic

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Publication Date
Sun Aug 01 2021
Journal Name
Nano Biomedicine And Engineering
In-vivo and In-vitro Anti-Acinetobacter Baumannii Activity of Citrate-Capped Silver Nanoparticles
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Silver nanoparticles (AgNPs) are of potential interest because of their effective antibacterial and antiviral activities. Capping agents are used for exhibiting a better antibacterial activity than uncapped Ag NPs. There are very few reports that have shown the usage of AgNPs for in-vivo antibacterial therapy. Citrate-capped silver nanoparticles were synthesized chemically by citrate reduction method; the size of Cit-AgNPs was determined by an atomic force microscope (AFM) and was between 15-90 nm. Acinetobacter baumannii (A. baumannii) isolates were the only sensitive species to Cit-AgNPs. MICs and MBC of Cit-AgNPs were determined by using A. baumannii. The results showed an additive effect of Cit-AgNPs. Four mice groups were infected with

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Publication Date
Fri Mar 01 2024
Journal Name
Baghdad Science Journal
Antimicrobial Activity of Silver Nanoparticles on Pathogenic Bacteria
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Nosocomial infection is acquired contamination of hospitals and health care units caused by multidrug resistant bacteria. Currently, bacterial resistance to antimicrobial medication represents a complicated public health problem. Recent studies on the antimicrobial activity of silver nanoparticles (AgNPs) attracted researchers worldwide to focus on the safe synthesis of AgNPs as antimicrobial agents against multidrug resistant bacteria. The antimicrobial efficacy of AgNPs on pathogenic bacteria isolated from clinical cases of acquired hospital infection was targeted in this project. Fifty specimens of stool were collected through private laboratories in Baghdad from patients who suffered diarrheal symptoms. Bacterial isolation, identific

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Publication Date
Mon Dec 30 2024
Journal Name
Iraqi Journal Of Science
Bio Synthesis, Characterization, and Evaluation of the Anticancer Activity of Gold and Silver Nanoparticles and Their Polyacetal Nanocomposite
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This study synthesized polyacetal from the reaction of polyvinyl alcohol with para-nitrobenzaldehyde. Polyacetal/polyvinylpyrrolidone polymer blends were prepared using solution casting. Gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) were biosynthesized using onion peel extract as the reducing agent. Nanocomposites were fabricated by blending polyacetal/PVP with AuNPs and AgNPs at different ratios. XRD and FESEM characterized the AuNPs and AgNPs. FTIR, FESEM, TGA, and DSC characterized the polyacetal, polymer blends, and nanocomposites. DSC and TGA confirmed the improved thermal stability of the polymer blends and nanocomposites. Nanocomposites demonstrated higher efficacy in inhibiting lung cancer cell lines compared t

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Publication Date
Mon Dec 05 2016
Journal Name
Iraqi
Synthesis, spectroscopic and Antibacterial activity study of N-]2(2-phenyl hydrazinyl) phenyl benzothiazol-2-amine complexes.
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Publication Date
Fri Jan 01 2016
Journal Name
The Pharma Innovation
Preparation, spectroscopic study of Schiff base ligand complexes with some metal ions and Evaluation of antibacterial activity
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Publication Date
Wed Apr 13 2016
Journal Name
The Pharma Innovation Journal
Preparation, spectroscopic study of Schiff base ligand complexes with some metal ions and Evaluation of antibacterial activity
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The new bidentate Schiff base ligand namely [(E)-N1-(4-methoxy benzylidene) benzene-1, 2-diamine] was prepared from condensation of 4-Methoxy benzaldehyde with O-Phenylene diamine at 1:1 molar ratio in ethanol as a solvent in presence of drops of 48% HBr. The structure of ligand (L) was characterized by, FT-IR, U.V-Vis., 1H-, 13C- NMR spectrophotometer, melting point and elemental microanalysis C.H.N. Metal complexes of the ligand (L) in general molecular formula [M(L)3], where M= Mn(II), Co(II), Ni(II),Cu(II) and Hg(II); L=(C14H14N2O) in ratio (1:3)(Metal:Ligand) were synthesized and characterized by Atomic absorption, FT- IR, U.V-Vis. spectra, molar conductivity, chloride content, melting point and magnetic susceptibility from the above d

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