Estimation of Immunoglobulins and complements and Using Enzyme linked Immuno sorbant Assay in Identification of Vulvovaginal candidiasis

Amna N.  Jassim; Hadeel A.  Majeed; Kalid A. Habib

doi:10.21123/bsj.2009.6.3.427-431

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Publication Date

Sun Sep 06 2009

Journal Name

Baghdad Science Journal

Volume

6

Issue Number

3

DOI

10.21123/bsj.2009.6.3.427-431

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Estimation of Immunoglobulins and complements and Using Enzyme linked Immuno sorbant Assay in Identification of Vulvovaginal candidiasis

"Vulvovaginal Candidiasis

ELISA

Candida spp"

Amna N. Jassim

Hadeel A. Majeed

Kalid A. Habib

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This study was conducted to determine the Immuno – globulins and complements quantitatively. The result revealed that the concentration of Immunoglobulin M(IgM) was increased significantly in patient group comparing with control group . The concentration of complement protein C4 was increased significantly in patient group comparing with control group.IgG of Candida albicans was detected by using ELISA Technique, the result indicated also that this antibody was found in 628% of the women who infected with Vulvovaginal Candidiasis. The sensitivity and specificity of the test were 63% and 89% respectively.

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Publication Date

Sun Sep 07 2014

Journal Name

Baghdad Science Journal

Determination of Copper and Lead and Study their Poisonous Effects in Drinking Water in Baghdad

Lead determination

Spectrophotometry

1-(2-pyridylazo)-2-napthol

copper determination

Thkra A.

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The objective of this study is to determine the concentration of copper and lead (mg/L) in drinking water by using absorption spectrophotometic and Atomic Absorption spectrophotometric method from different area in Baghdad and with different intervals , The results show that the concentration of copper and Lead ( mgL) in tap water which remains motionless in plumbing system for following periods one hours, 3 hours, 6 hours, 12 hours, 24 hours, 7 days and 14 days are (1 , 2.2 , 4 , 5.3 , 7.5 , 10 and 16 mgL copper ) & ( 0.3, 0.5 , 0.8 , 1 , 2.5 , 3 , 3.8 mg /L lead ) respectively .from these results its clear that high levels of copper & Lead occur if tap water comes in contact with copper - lead plumbing and copper lead -containing fix

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Publication Date

Thu Aug 18 2022

Journal Name

Physica Scripta

NORM and hazard indices in soil and dates of palms groves in Baghdad Governorate, Iraq

Basim Khalaf

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AbstractDate palm (Phoenix dactylifera) is one of the world’s oldest cultivated fruit crops and belongs to the Arecaceae family. It originated in Mesopotamia (Iraq) in 4000 BC. Large areas of palm groves in Iraq produce various types of dates for internal consumption and export. Their cultivation has spread and has become a significant crop in the Arabian Peninsula, North Africa, and the Middle East. Date fruits are widely consumed in Iraq, and radiological monitoring of this crop is necessary as activity concentrations of 238U, 232Th, 40K, and 137Cs were measured in 12 soil samples and 12 date samples from ... Show More

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Publication Date

Wed Oct 07 2020

Journal Name

Indian Journal Of Forensic Medicine & Toxicology

Correlation of Toxoplasmosis Seroprevalence and Serum Level of Interleukin-10 in Iraqi Breast Cancer Women

Breast cancer

ELISA

interleukine-10

Iraq

Toxoplasma gondii

Maysoon

Zeina

Zahraa

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Toxoplasmosis is regarded as one of the most important global life-threatening diseases in immune-compromised people. The intracellular protozoon Toxoplasma gondii is the causative pathogen of toxoplasmosis. Aim of this study is to investigate the possible association between T. gondii infection and breast cancer (BC) in Iraqi women, also to assess the effect of T. gondiion interleukin 10 (IL-10) of the immune response. By ELISA method, blood samples from 81 women with breast cancer and 60 apparently healthy women have been examined for presence of anti-toxoplasmaantibodies, also the levels of serum IL-10 were estimated in these subjects. Results showed that women with BC had the highest prevalence rate of toxoplasmosis. The anti- T.gondii

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Publication Date

Fri May 15 2009

Journal Name

Journal Of Planner And Development

Study of Change in Regional Land Use Using Remote Sensing Technology and Geographic Information Systems / Study Area (Hawr Al Huweiza) for the Years 1973-1990-2004

تقنيات مكانية

صلاح يوسف

علي حسين

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his project try to explain the using ability of spatial techniques for land cover change detection on regional level with the time parameter and did select for explain these abilities study case (Hewaizah marsh ) . this area apply to many big changes with the time. These changes made action on characters and behaviors of this area as well as all activities in it . This Project concerting to recognize the Using importance of remote sensing and GIS Methodology in data collecting for the changes of land use and the methodology for the analyses and getting the results for the next using as a base data for development and drawing the plans as well as in regional planning .This project focus on practical

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Publication Date

Sun Dec 01 2013

Journal Name

Baghdad Science Journal

Flow injection analysis for the photometric determination of promethazine-HCl in pure and pharmaceutical preparation via oxidation by persulphate using Ayah 3SX3-3D solar micro photometer

Flow injection analysis

promethazine hydrochloride

spectrophotometric.

Issam M. A.

Nagam S.

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The first flow injection spectrophotometric method is characterized by its speed and sensitivity which have been developed for the determination of promethazine-HCl in pure and pharmaceutical preparation. It is based on the in situ detection of colored cationic radicals formed via oxidation of the drug with sodium persulphate to pinkish-red species and the same species was determined by using homemade Ayah 3SX3-3D solar flow injection photometer. Optimum conditions were obtained by using the high intensive green light emitted diode as a source. Linear dynamic range for the absorbance versus promethazine-HCl concentration was 0-7 mmol.L-1, with the correlation coefficient (r) was 0.9904 while the percentage linearity (r2%) was 98.09%. the L.

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Publication Date

Sat Dec 01 2018

Journal Name

Journal Of Accounting And Financial Studies ( Jafs )

" Measuring the competitive performance of the National Insurance Company and some of its branches using the Revealed comparative advantage index RCAI "

أ. م. حيدر شاكر

م. م. محمد إبراهيم

م. م. عامر رشيد

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The research dealt with the subject of measuring the competitive performance of the National Insurance Company and some of its branches (Basra, Ninwa, Kirkuk and Babil), Depending on the Revenue Growth Index at the activity level, and the Revealed Comparative Advantage Index RCAIAt the branch level,To measure the competitiveness of the company And some branches, As the problem of research in the lack of adoption by some companies in the insurance service sector on scientific indicators to measure their competitive performance, The aims of the research is to measure the competitiveness of the National Insurance Company, as well as the competitiveness of its branches according to the scientific method, One of the main Conclusions of the re

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Publication Date

Sun Jan 01 2023

Journal Name

Plant Archives

INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION AND GERMINATION OF DATE PALM CV BARHI BY USING CELL SUSPENSION CULTURE TECHNIQUEe

Moayad Rajab Abood

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INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION AND GERMINATION OF DATE PALM CV BARHI BY USING CELL SUSPENSION CULTURE TECHNIQUEe

Publication Date

Thu Oct 01 2020

Journal Name

August

INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION AND GERMINATION OF DATE PALM BARHI C.V BY USING CELL SUSPENSION CULTURE TECHNIQUE

Ali . A

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INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION AND GERMINATION OF DATE PALM BARHI C.V BY USING CELL SUSPENSION CULTURE TECHNIQUE

Publication Date

Fri Mar 27 2020

Journal Name

Plant Archives 20 (supplement 1), 1666-1670‏

INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION AND GERMINATION OF DATE PALM CV BARHI BY USING CELL SUSPENSION CULTURE TECHNIQUEث‏

Moayad Rajab Abood

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Publication Date

Mon Apr 06 2020

Journal Name

August

INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION AND GERMINATION OF DATE PALM CV BARHI BY USING CELL SUSPENSION CULTURE TECHNIQUE

This study was done to examine different concentrations of mercuric chloride (Hgcl2) for Explants sterilization and different concentration of NAA) mg/L with 2iP for their effect on initiation primary callus and subsequent embryogenesis in Phoenix dactyliferae cultivar Barhi. Leaf primordial and shoot tips were excised from 2-3 years old offshoot. The result showed that contamination percentage decreased with an increase in concentration of Hgcl2 for two explants where in the control (0.0)% gave highest value 75% and 62.5% of contamination for leaf primordial and shoot tip respectively while the addition (0.1)% gave a significant decrease 37.5 and 0% for explants respectively. The Explants were inoculated onto Murashiege and Skoog

1962 (MS) medium supplemented with 2iP 3mg/L and containing 0

25

50 or 100 mg/L of NAA

the addition (50) mg/L of NAA gave highest value 68.8% in percentage of callus initiation with significant difference from addition 0% gave lowest value (18.8)%

as well Explants of shoot tip gave significant difference was 53.1% while leaf primordial gave 28.1%

Primary callus was transferred to fresh MS medium containing (0

5

10 and 20) mg/L of NAA with 3mg/L 2iP

Results were recorded after 12 weeks. A highest value of embryonic callus induction percentage or fresh weight and dry weight 87.5% or 1.56g and 0.47g for the character respectively for the treat 20 mg/L while control treatment 0.0 gave the lowest value 12.5% or 0.37g and 0.14g for same character respectively. Inoculated 1g fresh biomass into a 250 ml Erlenmeyer flask containing 50 ml MS liquid medium supplemented with NAA 20mg/L and agitated at 150 rpm by shaker to making suspension culture the measurements of packed cell volume ( pcv) or fresh and dry weight taken weekly for 12 weeks

maximum values were reached in the eighth week of PCV and fresh weight but the dry weight reached to highest value in seventh week

then the curve began to decline gradually until it reached stability in the last two weeks. The cell suspension was Diluted to different initial cell density of 100

500

10000

50000 and 100000 cells /ml with hemocytometer. The initial cell number of (10000) cells gave significant different of plating efficiency PE (11.6)% while (100) cells gave lowest value 1.8% of PE.

Ali A.

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