Acute myeloid leukemia (AML) represents the most prevalent type of acute leukemia in adults and is responsible for approximately 80% of all cases. The tumor suppressor gene (TP53) is a gene that has been frequently studied in cancer, and mutations in this gene account for about 50% of human cancers. This study aims to evaluate the correlation between two single nucleotide polymorphisms (SNPs) in the gene: rs1042522 and rs1642785, and a group of Iraqi patients suffering from pre-diagnostic acute myeloid leukemia (AML). Blood samples were collected from sixty patients (26 males and 34 females) and sixty controls (26 males and 34 females); these subjects were matched in gender, age, and ethnicity. Genomic DNA has been extracted from whole frozen blood samples by using the Easy Pure® Blood Genomic Kit, and then the purity and concentration have been measured by using the Nano drop NAS-99 spectrophotometer. Nano Drop readings ranged between 7-55ng/µl for the concentration and between 1.78-1.9 for the purity. High resolution melt (HRM) real-time PCR was used in the detection of these two SNPs. TP53 genotype frequencies have been in accordance with Hardy–Weinberg equilibrium (HWE), with statistically significant differences p≤0.05 between the genotypes of the control and patient groups. The rs1042522 genotype frequency was significantly different between patients and controls p = 0.0001, and participants with the GA genotype were more likely to develop AML OR = 7.8, 95% CI 3.2–18.4, p= 0.0001. In addition, the genotype frequency of rs1642785 was significantly different between patients and controls p = 0.002, and participants with the GA genotype were more likely to develop AML OR = 3.5, 95% CI 1.5–8.12, p = 0.002.
Resumen
El trabajo se investiga en el realismo y la tradición social que incluye la tragedia Bodas de sangre, del autor español Federico García Lorca. A lo largo de estas páginas se da la luz sobre los temas, acciones, oraciones y palabras que pertenecen a la realidad de la vida española en general y sobre todo en territorio andaluz, en el que ocurren los sucesos de la obra. Así como las tradiciones sociales que el dramaturgo español contemporáneo abundando a lo largo de su texto, y que han sido tomadas de la herencia popular.
Literariamente, esta obra se clasifica como Tragedia Moderna, por la razón de que incorpora los elementos necesarios de d
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Die Übersetzung gilt in der Vergangenheit als Brücke zwischen verschiedenen Gesellschaften. Sie spielt eine große Rolle im Bereich Kulturaustausch der Länder. Die Araber haben das früh betrachtet, und die Übersetzung als Wissenschaft wird eine der wichtigsten Lehren. Viele griechische Wissenschaften waren durch Übersetzung in den arabischen Ländern bekannt.
In dieser Forschung findet man einen Überblick über die Wichtigkeit der Sprichwörterübersetzung und welche Rolle spielt diese Übersetzung im Z
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