Gingival crevicular fluid (GCF) may reflect the events associated with orthodontic tooth movement. Attempts have been conducted to identify biomarkers reflecting optimum orthodontic force, unwanted sequallea (i.e. root resorption) and accelerated tooth movement. The aim of the present study is to find out a standardized GCF collection, storage and total protein extraction method from apparently healthy gingival sites with orthodontics that is compatible with further high-throughput proteomics. Eighteen patients who required extractions of both maxillary first premolars were recruited in this study. These teeth were randomly assigned to either heavy (225g) or light force (25g), and their site specific GCF was collected at baseline and after 1hr, 1day, 7days, 14days, 21days and 28days post force application. Periostrips were used for GCF collection and subsequent phosphate buffered saline (PBS) was used for immediate protein elution with centrifugal speed of 10000rpm for 5min and stored at -80°C. Protein concentration was estimated using Bradford colorimetric assay. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was carried out to resolve the purity of proteins in the collected samples and the method of collection was validated by western immuno-blotting of alpha amylase salivary enzyme. The current collection, storage and protein extraction protocol showed the best protein recovery and purity with validated collection free of salivary contamination. In conclusion, tiny GCF volume from healthy sites and evaporation issues of such promising non-invasive fluid motivate us to investigate a standardized protocol enabling optimal preservation of GCF sample and the currently followed protocol may serve as a reference for future proteomic studies searching for GCF biomarkers in diagnosing and monitoring orthodontic tooth movement.
تقدمت احداث النصف الاخير من القرن الماضي من تاريخ العالم الاقتصادي صور كثيرة من التكتلات والاليات التعاونية والتشاركية القائمة على مصادر مختلفة للتجمع الاقتصادي من اهمها شكل الشراكات التجارية والاقتصادية ولها امثلة كثيرة على المستوى الاقتصادي الدولي ومنها الشراكة الاورومتوسطية التي تمثل منطقة حيوية في حسابات التبادل الدولي تجاريا وراسماليا ولكن هذا التشكيل التكتلي يحتاج الى وقفه تخص
... Show MoreThis study aims at investigating the partial Islamic rules of preparing and distributing cartoons in order to issue an overall Islamic rul. To reach an end, descriptive and analytical approaches are adopted to clarify the nature of cartoons and other related concepts. The researcher, as well, with reference to verses of the Holy Quran, tradition (Hadith) and Islamic jurists, adopts a deductive approach to issue Islamic rules related to the industry of cartoons and it's distribution
The study consists of three sections. The first Section addresses the following issues: Definition animation; and related wordy. The second Section: Origin of Cartoon's history and it's negative and positive effects. The third Section: Islamic rules related
The research aims to introduce the elders of Imam Al-Ala who died in the year 761 AH. The study showed the prominent role of women in the eighth century AH in the sciences in general and in the hadith in particular. The woman did not depart from the call of divine education in the Almighty saying: Read in the name of your Lord who created * created mankind * created mankind * And your Lord, the Most Generous, who taught with the pen * taught man unless he knows the captive of verses 1-5.
Families in that era had a prominent role in this concern, especially parents. They were attending boys and girls since their childhood, the lessons of scholars, and the benefit of science, and they were
Dada la importancia de la gramática en las lenguas hemos tratado en esta investigación manifestar la importancia de la posición del adjetivo en la lengua castellana y hacer una simple comparación con la lengua inglesa y la lengua árabe.
Los comportamientos de los adjetivos en lo referente a su posición respecto del sustantivo no siguen una fórmula fija. En esta cuestión “no se trata de leyes, sino de tendencias”, como dice Gili Gaya1, la inmensa mayoría de los adjetivos del español son susceptibles de ser colocados antes o después del sustantivo al que sirvan de adjunto. Las formulaciones sobre el orden Sust.+Adj. o el orden Adj.+Sust., por muy genéricas que sean, sufren excepciones,según las preferencias contextualiz
An ancient iraq man lived under sever shadow which led him to be creative for providing instruments that ables him to keep his life with who are near to him, this matter led to appear humanity civilization
This research is dealing with Islamic creed faith which would be the prophecy concept. The prophecies subject one greatest creed doors with God’s - high ranking – fortunate - messengers faith is one faith’s six pillars ; though no correct faith to adorer , until the adored completely faith in God’s angels , holly books
The main goal of this paper is to introduce and study a new concept named d*-supplemented which can be considered as a generalization of W- supplemented modules and d-hollow module. Also, we introduce a d*-supplement submodule. Many relationships of d*-supplemented modules are studied. Especially, we give characterizations of d*-supplemented modules and relationship between this kind of modules and other kind modules for example every d-hollow (d-local) module is d*-supplemented and by an example we show that the converse is not true.
The nuclear charge density distributions, form factors andcorresponding proton, charge, neutron, and matter root mean squareradii for stable 4He, 12C, and 16O nuclei have been calculated usingsingle-particle radial wave functions of Woods-Saxon potential andharmonic-oscillator potential for comparison. The calculations for theground charge density distributions using the Woods-Saxon potentialshow good agreement with experimental data for 4He nucleus whilethe results for 12C and 16O nuclei are better in harmonic-oscillatorpotential. The calculated elastic charge form factors in Woods-Saxonpotential are better than the results of harmonic-oscillator potential.Finally, the calculated root mean square radii usingWoods-Saxonpotentials ho
... Show MoreThe research aims to identify small and medium enterprises in accounting thought in terms of definition and concept, and this international financial reporting standard for small and medium-sized enterprises (SMEs) in the theoretical aspect. As for the practical aspect, the small and medium-sized enterprises standard has been applied to the financial statements of the company in question, the preparation of the opening entry on the date of the transition, the requirements of measurement and the accounting disclosure on the date following the application of the standard, and the preparation of the company's financial statements and the accompanying explanations according to the standard of small and medium-sized enterprises. The r
... Show MoreThe nuclear charge density distributions, form factors and
corresponding proton, charge, neutron, and matter root mean square
radii for stable 4He, 12C, and 16O nuclei have been calculated using
single-particle radial wave functions of Woods-Saxon potential and
harmonic-oscillator potential for comparison. The calculations for the
ground charge density distributions using the Woods-Saxon potential
show good agreement with experimental data for 4He nucleus while
the results for 12C and 16O nuclei are better in harmonic-oscillator
potential. The calculated elastic charge form factors in Woods-Saxon
potential are better than the results of harmonic-oscillator potential.
Finally, the calculated root mean square