Preferred Language
Articles
/
bsj-5067
Biochemical and Histological Study of Aminoacylase-1 Purified from Amniotic Fluid in Rats with Oxidative Stress Induced by Lead Acetate
...Show More Authors

This work involves separating and studying the aminoacylase-1 (ACY1) of amniotic fluid from healthy pregnant, mainly one peak with higher activity has been isolated by DEAE-Cellulose ion exchange from the proteinous supernatant produced by deposition of proteins using ammonium sulfate  (65%) after dialysis. The purification folds reaching to 19 folds also gave one protein peak when injected into the gel filtration column, a high ACY1 purity was obtained, with 38 folds of purification. It was found that the molecular weight of the isolated ACY1 was up to 46698 Dalton when using gel chromatography technique.The effect of ACY1 isolate was studied on rats with oxidative stress caused by lead acetate(LA) at 40 mg / kg body weight and compared with normal rats by measuring the selected biochemical parameters which included: Glutathione (GSH), malondialdehyde (MDA), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) through liver and kidney tissue examination. The results showed a significant increase in the levels of (MDA, AST, ALT) and a decrease in the level of GSH compared with the control group, Also it has been observed there that was a significant decrease in the levels of (MDA, AST, ALT) and high level of GSH when injecting the ACY1 isolate in a dose of 4 mg / kg of rat weight with LA at 40 mg/kg. The results of the tissue examination demonstrated high pathological changes in the liver tissue of rats treated with LA at 40 mg/kg of rat weight when compared with normal rats. The liver and kidney tissue improved when treated with isolate at 4 mg / kg rat weight and LA. These results demonstrate the role of ACY1 in protecting from oxidative stress then can reduce the severity of various diseases.

Studying the effect of ACY1 isolated on rats with oxidative stress caused by lead acetate at a dose of 40 mg / kg body weight and compared with normal rats by measuring the selected biochemical parameters which included: Glutathione (GSH), malondialdehyde (MDA), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) as well as through liver and kidney tissue examination. The results showed a significant increase in the levels of (MDA, AST, ALT) and a decrease in the level of GSH compared with the control group, It was also observed that there was a significant decrease in the levels (MDA, AST, ALT) and high level of GSH when using the enzyme isolated in a dose of 4 mg / kg of rat weight with lead acetate at a dose of 40 mg/kg. The results of the tissue examination showed high pathological changes in the liver tissue of rats treated with lead acetate at a dose of 40 mg/kg of rat weight when compared with normal rats, and liver and kidney tissue improvement when isolated enzyme is administered at 4 mg / kg rat weight with lead acetate. These results demonstrate the role of isolated enzyme in protecting the body from oxidative stress then can reducing the severity of various diseases.

Scopus Clarivate Crossref
View Publication Preview PDF
Quick Preview PDF
Publication Date
Mon Jun 01 2020
Journal Name
Journal Of Engineering
Improve Rheological Properties of Palygorskite Water-Based Drilling Fluid by Caustic Soda and Soda Ash
...Show More Authors

In drilling fluid program, selecting the drilling fluid that will reduce the lost time is the first objective, and will be economical regardless of its cost. The amount and type of solids in drilling fluid is the primary control of the rheological and filtration properties. Palygorskite clay (attapulgite) is an active solid that has the ability to reactive with its environment and form a gel structure within a fluid and due to its stability in the presence of brines and electrolytes this type of clay is preferred for use. The aim of this study is to improve properties of Iraqi palygorskite (PAL) by adding different chemical additives such as caustic soda NaOH and soda ash Na2CO3 with a different con

... Show More
View Publication Preview PDF
Crossref (7)
Crossref
Publication Date
Sun Jun 07 2015
Journal Name
Baghdad Science Journal
Morphological and Histological study of pancreas in (Saw-scaled viper) Echis carinatus scochureki (Stemmler,1969)
...Show More Authors

The aims of study were identify the Morphological description and histological structure of pancreas gland in the Saw-scaled viper snake, (Echis carinatus scochureki). The study was conducted on 6 snakes collected from the city of Nasiriya in Dhi Qar province, Doped snakes was dissected to isolate the pancreas gland, and the histological slides prepared after samples fixation by fixative solutions, the routine stains Haematoxylin– Eosin were used. Morphological Description study showed that the pancreas gland was compact in type with one lobe, white-pink in colure. The pancreas Located at the level of the Gall bladder associated with it’s caudal side, The spleen is adjacent to pancreas completely in the dorsal side .and duodenum l

... Show More
View Publication Preview PDF
Crossref
Publication Date
Sat May 01 2021
Journal Name
Journal Of Physics: Conference Series
Morphological, histochemical and histological study of the ileum in the Iraqi Black Partridge (Francolinus francolinus)
...Show More Authors
Abstract<p>Objective: This project aimed to study the morphological description and histological structure of ileum in Iraqi black partridge (<italic>Francolinus francolinus</italic>). Methods: To conduct this investigation, 20 healthy Iraqi black partridge were collected from local suppliers. Birds were euthanized, dissected and then specimens were processed for histological and histochemical staining techniques. Results: A morphological study showed that the ileum appears as a short narrow tubular structure. It extends from the wide end of the jejunum and ends in the Ileo-caecal junction region. Histologically, the wall ileum consists of four tunica mucosa, submucosa, muscularis and </p> ... Show More
View Publication
Scopus (4)
Crossref (1)
Scopus Crossref
Publication Date
Wed Jun 01 2022
Journal Name
Resmilitaris
Evaluation mucin 1 polymorphism and expression with infertility in Iraqi females
...Show More Authors

Scopus (2)
Scopus
Publication Date
Thu May 04 2023
Journal Name
Biomedicine
Relationship between cathepsin K and total oxidative state in diabetes mellitus female patients with osteoporosis in Iraq
...Show More Authors

Introduction and Aim: Diabetes mellitus patients almost always struggle with a metabolic condition known as chronic hyperglycemia. According to the World Health Organization, osteoporosis is a progressive systemic skeletal disorder that is characterized by decreasing bone mass and microstructural breakdown of bone tissue that increases susceptibility to fracture and increased risk of breaking a bone. Here, we aimed to compare the levels of CatK and total oxidative state in patients with diabetes and osteoporosis among the female Iraqi population and study the possible relationship between them.   Materials and Methods: This study included 40 females with diabetes (Group G1), 40 with diabetes and osteoporosis (Group G2) and 40 norma

... Show More
View Publication
Scopus (1)
Scopus Crossref
Publication Date
Mon Feb 24 2025
Journal Name
Baghdad Science Journal
Porosity Measurements of Positive of Lead-Acid Battery Plates by Mercury PSorosimetry
...Show More Authors

A mercury porosimeter has been used to measure the intrusion volume of the three types mercury positive lead acid-battery plates. The intrusion volumes were used to calculate the pore diameter, pore volume, pore area, and pore size distribution. The variation of the pore area in positive lead acid-battery plates as well as of the pore volume has the following sequence. Paste positive > Uncured positive > Cured positive

View Publication
Crossref
Publication Date
Sun Jun 05 2016
Journal Name
Baghdad Science Journal
Bioremediation of Lead and Cadmium Contaminated soil by Sesbania rostrata plant and AM fungi Glomus mosseae
...Show More Authors

This study was conducted to determine the activity of plant Sesbania rostrata and two isolate from arbuscular mycorrhizae fungi (A,B) as a bioremediation of soil polluted by cadmium and lead elements in north and south of Baghdad city. The results showed that the average of soil pollution by cadmium and lead elements in north of Baghdad was less than the average of soil pollution in the south of Baghdad which recorded 10.0, 9.0 mg/kg and 27.0, 25.0 mg/kg respectively. The plant Sesbania recorded ability to accumulate the lead element in shoot system 19.65 mg/kg and in root system 27.2 mg/kg and for cadmium element 19.6, 24.6 mg/kg in shoot and root respectively. The results showed that the isolate A from soil pollution is more effected

... Show More
View Publication Preview PDF
Crossref
Publication Date
Sat Sep 30 2017
Journal Name
College Of Islamic Sciences
Ruling on questioning children by the security authorities: (Comparative Study between Sharia and Law)
...Show More Authors

Some of the issues that have become common in our society recently after the Americans entered our country and were rubbed by some security agencies: obtaining some information from children, and the serious consequences that may lead to the lives of innocent people, became common interrogation of some security agencies and rely on their words.
There are significant cases where their testimony needs to be heard, such as their presence in some places where incidents are not witnessed by others, such as schools or being witnesses to certain crimes.
I saw the study of this case in the light of Sharia and law

View Publication Preview PDF
Publication Date
Sat Jul 30 2022
Journal Name
Gsc Biological And Pharmaceutical Sciences
Histological study of liver for squirrel (Sciurus anomalus) (Güldenstädt, 1785) in Iraq
...Show More Authors

The liver (hepatic) is one of the largest glands or organs of the digestive system in the body of living organisms, including rodents, take the squirrel in this study for example. The study and the collection of sources emerged to be put into the hands of those, especially those interested in histological studies, including junior or professionals and veterinarians, knowledge of the stains used in the research and their final results.

View Publication
Crossref (1)
Crossref
Publication Date
Thu Jan 01 2009
Journal Name
Arab Journal Of Gasteroenterology
Serum HCV-RNA levels in patients with chronic hepatitis C: correlation with histological features.
...Show More Authors