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bsj-1433
Molecular Diagnosis of Brucella species in Baghdad
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Brucellosis is possess a significant public health problem in Baghdad. In this study, we investigated the potential role of the PCR assay in detection of Brucella species, from patients suspect to have brucellosis, using blood samples in both human and animal. To establish a PCR technique for diagnosis of active brucellosis in our samples, DNA extraction was carried out using a commercial kit, and a laboratory extraction procedure. PCR amplification was done using 1 set of primers: B4/B5 for Brucella species. Extraction of Brucella DNA using the commercial kit was successful. The laboratory extraction was successful and more economic. A total of 178 peripheral blood specimens were collected from patients with high suspected brucellosis, and 15 samples from animals. When PCR technique was applied to blood samples, 13 cases for patients blood and 9 cases for animals blood, were positive for Brucella species.

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Publication Date
Sun Jan 01 2017
Journal Name
World Journal Of Pharmaceutical Research
IDENTIFICATION OF SOME SPECIES OF WIREWORM AGRIOTES SPP. (COLEOPTERA: ELATERIDAE) IN BAGHDAD
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Publication Date
Sun Jun 01 2014
Journal Name
Baghdad Science Journal
Pristina species (oligochaeta: naididae) in tigris river within baghdad city / Iraq
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A total of 72 individuals of genus Pristina were sorted from aquatic plant, Ceratophyllum demersum L., and filamentous algae collected from three sites on Tigris River at Baghdad including: Al-Sarafiya area (S1), Al- Jadiriyah area (S2), and Al- Za´afaraniya area (S3). Four species were identified including P. longiseta, P. aequiseta, P. proboscidea and P. foreli, with percentags of 51.7 , 36.4, 1.1, and 10.5 % respectively. The first two species found in all sites , while , P. proboscidea found only in S1 and P. foreli only in S2.

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Publication Date
Mon Sep 28 2026
Journal Name
Philippine Journal Of Veterinary Medicine
Traditional Molecular Study of Eimeria Species (Eucoccidiorida, Eimeriidae) from Ostriches (Struthio camelus Linnaeus, 1758) in Middle of Iraq
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Abstract Background: Coccidiosis in birds, including ostriches, is a major concern for animal health and productivity worldwide. There is limited data on Eimeria infections in farmed ostriches (Struthio camelus). Therefore, this research aims to investigate the species of Eimeria in ostriches using molecular methods. It is considered the first detailed molecular study of Eimeria species in Iraqi ostrich farms. Methods: A total of 150 ostrich feces samples from farms in three regions of Iraq using both microscopy and molecular methods. Statistical analysis of the positive samples was performed using the chi-square test, with the significance level set at a p-value < 0.05. Results: Initial testing showed a high prevalence rate of 51.33%. Five

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Publication Date
Wed Jan 01 2025
Journal Name
Journal Of Advanced Veterinary And Animal Research
Investigation and genetic confirmation of the Cryptosporidium species in fish handlers in Baghdad city
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Objective: The present study aims to investigate molecular confirmation for <i>Cryptosporidium</i> species in fish handlers in Baghdad City, central Iraq. Materials and Methods: Sixty stool samples were collected between early November 2023 and late April 2024. All samples were examined phenotypically using a modified Ziehl-Neelsen stain and genotypically (nested polymerase chain reaction technique) based on a partial sequence of 18S rRNA genes with sequencing and phylogenetic tree analysis. Results: The total molecular results identified <i>Cryptosporidium parvum</i> with an infection rate of 45% (27/60). A higher infection rate of 51.9% (14/27) was found in the age group between 15 and 35 years, and male

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Publication Date
Fri Mar 19 2021
Journal Name
Annals Of The Romanian Society For Cell Biology
Molecular Detection of Acientobacter Baumannii Isolated From Nosocomial Infections in Baghdad Hospitals
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&nbsp;The present study is an attempt for detection of A. baumannii by conventional and PCR methods using species-specific primers for these A. baumannii. A total of 87 samples were collected from hospitals in Baghdad (Al-Rasafa and Al-Karkh Hospitals) during the period from 2019 to 2020.The samples included: 40 specimens, from wounds, respiratory infections (sputum), burns, CSF and 47 samples from the hospital environment (swabs), while samples collected from intensive care unit including patient beds, surgical instruments and appliances, emergency lobby and baby incubators. A. baumannii isolate identification depending on the morphologic characteristics on the culture media including, blood agar, MacConkey&nbsp; agar, as well as t

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Publication Date
Sat Oct 02 2010
Journal Name
Journal Ibn Al-haitham Journal For Pure And Applied Sciences
Seasonal abundance of adult beetle species on the exposed carcasses in Baghdad City
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Publication Date
Wed Apr 01 2009
Journal Name
Ibn Al-haitham Journal For Pure And Applied Science
Seasonal Abundance of Adult Fly Species on the Exposed Carcasses in Baghdad City
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Adult of dipterous flies were collected monthly from exposed animals carcasses during the period from February 2006 to January 2007 in Baghdad city. The results obtained showed that flies could be collected all over the year with variation of their population density in different seasons. The majority of the collected species were abundant during Spring and late Autumn (at lowest numbers) . In this investigation, nineteen species confined to four families were collected; these families are: Calliphoridae, Muscidae, Sarcophagidae and Fanniidae. The species Musca domestica Linn .was the most abundant followed by Chrysomya megacephala ( Fabricius ) , while Pollenia sp . and Fannia sp . were the least abundant species.

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Publication Date
Thu Dec 31 2009
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Seasonal abundances of adult fly species on the exposed carcasses in Baghdad City
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Publication Date
Thu Jul 01 2010
Journal Name
Bulletin Of The Iraq Natural History Museum (p-issn: 1017-8678 , E-issn: 2311-9799)
KEYS FOR GENAR AND SPECIES OF FAMILY PHLEAOTHRIPIDAE (TUBUILIFERA: THYSANOPTERA) IN BAGHDAD-IRAQ
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Keys to four genera and twelve species of the subfamily Phlaeothripinae (Phlaeothripidae) were constructed, these are: Haplothrips; Karnyothrips; Phlaeothrips; and Dolicholepta ,and the species are: Haplothrips cerealis Priesner; Haplothrips tritici kurdjumov, Haplothrips hukkineni Priesner; Haplothrips subtilissimus (Haliday) ؛ Haplothrips reuteri Karny; Haplothrips jasonis Priesner; Haplothrips sallloumensis Priesner ; Haplothrips pharao Priesner ; Phlaeothrips sycomri Priesner ; Karnyothrips flavipus (Jones); Karnyothrips melaleucus (Bagnall) ; Dolicholepta micrurus (Bagnall). These were collected from Baghdad

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Publication Date
Fri Sep 01 2023
Journal Name
Advancements In Life Sciences
Investigation of pathogenic Streptococci species in local and imported mozzarella soft cheese in Baghdad city
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Background: The present work investigated the profile and biodiversity of the pathogenic streptococci species isolated from local and imported mozzarella soft cheese in Baghdad City from October 2022 to January 2023. The study aimed to examine the molecular characterization of 16Sr RNA gene in some streptococcus species isolates from mozzarella soft cheese in Baghdad city Methods: From 50 samples, 8 isolates in all were found and identified based on the VITEK, molecular, and sequencing of the 16SrRNA gene. The eight isolates represented Lactococcus cremoris. Streptococcus alactolyticus, Streptococcus sanguinis, and Streptococcus thoraltensis. The isolates were subjected to conventional PCR and electrophoresis to detect the 16SrRNA gene usi

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