Influence of Indole acetic acid and Tryptophan on production   of Vinblastine and Vincristine of  Catharanthus roseous callus cells in the accumulation media of In Vitro tissue culture

Sabah Mehdi  Hadi

doi:10.21123/bsj.2010.7.3.1113-1119

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Publication Date

Sun Sep 05 2010

Journal Name

Baghdad Science Journal

Volume

7

Issue Number

3

DOI

10.21123/bsj.2010.7.3.1113-1119

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Influence of Indole acetic acid and Tryptophan on production of Vinblastine and Vincristine of Catharanthus roseous callus cells in the accumulation media of In Vitro tissue culture

"???? ??? ???? ??

????????? ?????????

????? ????? ??????. MS: Murashige and Skooge IAA : indolyl-3-acetic acid NAA: naphthalene acetic acid 2

4-D: 2

4-dichlorophenoxy acetic acid"

Sabah Mehdi Hadi

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This study on the plant of Ain –AL Bason Catharanthus roseous showed the ability of callus cells that is produced by In Vitro culture technique and transformed to the accumulated media (MS 40gm/L sucrose ,2gm/L IAA Indole acetic acid , 0.5gm/L Tryptophan) to produce Vinblastine and Vincristine compounds. Extraction, purification and quantitive determination of Vinblastine and Vincristine compounds using High performance liquid chromatography technique (HPLC)were carried out. The results showed that the highest concentration of Vinblastine and Vincristine compounds were ( 4.653,12.5 (ppm /0.5 dry Wight respectively from transformed callus cells from MS 40 gm /L sucrose , 2 gm / L NAA Naphthaline acetic acid .

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Publication Date

Thu Jun 25 2020

Journal Name

Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)

Synthesis, Characterization and Antibacterial Activity Evaluation of New Indole-Based Derivatives

N-acyl hydrazone

Indole-3-propionic acid

antibacterial.

Ahmed T.

Susan

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A new series of N-acyl hydrazones (4a-g) derived from indole-3-propionic acid (IPA) were synthesized. These N-acyl hydrazones were prepared by the reaction of 3-(1H-indol-3-yl) propane hydrazide and aldehyde in the existence of glacial acetic acid as a catalyst. ¹HNMR and FT-IR analyses were used to identify the synthesized compounds and they were in vitro evaluated as antibacterial agents against six different types of microorganisms by using well diffusion method. All the tested N-acyl hydrazones (4a-g) displayed moderate activity against the Gram-negative E.coli, comparable to that of Amoxicillin. Some of the tested N-acyl hydrazones also exhibited intermediate activity ag

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Publication Date

Thu Apr 01 2021

Journal Name

Biochemical And Cellular Archives

Improvement in the Growth of Cumin Plant, Cuminum Cyminum L. with Spraying Tryptophan and Iq Combi Nanofertilizer

Cumin plant

amino acids

tryptophan

nanotechnology

AL-Kazzaz A.G.M.

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The experiment was carried out in the Department of Biology, College of Education for Pure Science –Ibn AL Haitham, University of Baghdad, Iraq, during the growing season 2017 – 2018. The objective was to find out the effect of foliar spraying of tryptophan and IQ COMBI nano fertilizer on cumin plants. The obtained results show that both tryptophan and IQ COMBI nano fertilizer increased plant height, root length, shoot dry weight, the content of nitrogen, phosphorus, potassium, protein percentage, no. compound umbel.plant-1, wt. seeds. plant-1. The optimum treatment combination was calculated as 30 mg.L-1 tryptophan, 1000mg.L-1 IQ COMBI nano fertilizer, which gave the highest values for most of the parameters studied

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Publication Date

Fri Dec 01 2023

Journal Name

Asian Pacific Journal Of Cancer Prevention

Effect of Laetrile Vinblastine Combination on the Proliferation of the Hela Cancer Cell Line

azal hamoody

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Publication Date

Wed May 01 2013

Journal Name

Chemical And Process Engineering Research

Synthesis and Characterization of Polycyclicacetal Derived from PEG & Erythro-Ascorbic Acid Derivative and Study Its Effect on the Activity of ACh Enzyme (In Vitro)

Polycyclicacetal

PEG

Acetylcholinesterase.

Maha A

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Polycyclicacetal was prepared from the reaction of PEG with aldehyde derived from Erythro-ascorbic acid (pentulosono-ɣ-lactone-2,3-enedianisoate).All these compounds were characterized by Thin Layer Chromatography (TLC) and FTIR spectra and aldehyde was also characterized by (U.V-Vis), 1HNMR, 13CNMR, and mass spectra.The inhibitory effect of prepared polymer on the activity of human serum AcetylCholinesterase has been studied in vitro. The polymer showed a remarkable activity at low concentration (4.7x10-3 – 4.7x10-8M).

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Publication Date

Sun Sep 01 2019

Journal Name

Baghdad Science Journal

Efficiency of Green Algae Chlorella vulgaris in Remediation of Polycyclic Aromatic Hydrocarbon (Anthracene) from Culture Media

Anthracene

Bioremediation

Chlorella vulgaris

Green algae

Polycyclic aromatic hydrocarbon(PAHs).

Juda

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The fresh water green alga Chlorella vulgaris was selected to study its ability to degrade one of polycyclic aromatic hydrocarbon (PAHs)known is anthracene (ANT). ANT is widely used in artificial products such as wood preservatives; insecticides; dye and coating materials. This algae was cultivated in CH-10 medium under constant laboratory conditions and exposed to different concentrations (1, 3, and 5 mg/l) of anthracene for 3, 5, 7, 9 and 15 days, with the concentration of ANT measured by high performance liquid chromatographic analysis (HPLC). The results showed that C.vulgaris has high ability to reduce anthracene to 80% at 1mg/Lcon centration after 3 days and 100% after 5 days, while at 3 and 5 mg / L concent

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(5)

(1)

Publication Date

Mon Sep 02 2024

Journal Name

Journal Of The American Oil Chemists' Society

Direct application of tungstosilicic acid hydrate for the treatment of high free fatty acid in acidic crude palm oil and for biodiesel production

Khalid M.

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Abstract<p>This study explored the use of industrial acidic crude palm oil (ACPO) for biodiesel production, facing a significant obstacle due to its high free fatty acid (FFA) content, which complicates the biodiesel production process. Typically, esterification is employed to convert FFAs into fatty acid methyl ester (FAME). Herein, the effectiveness of tungstosilicic acid hydrate (TSAH) as an unsupported heteropoly acid (HPA) catalyst for FFA esterification in ACPO was investigated. The FFA content was reduced from 8.43% to 0.95% under optimum conditions (4 wt% catalyst dosage, a methanol to oil molar ratio of 10:1, 150 min and a temperature of 60°C). Noteworthy, the TSAH catalyst showed stability over 7</p> ... Show More

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(2)

Publication Date

Sun Jan 01 2023

Journal Name

Journal Of Clinical And Experimental Dentistry

Preventive effect of combined Er, Cr: YSGG and fluoride gel on acid resistance of the permanent tooth enamel: An in vitro study

Dhuha

AM.

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(5)

Publication Date

Mon Apr 06 2020

Journal Name

August

INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION AND GERMINATION OF DATE PALM CV BARHI BY USING CELL SUSPENSION CULTURE TECHNIQUE

This study was done to examine different concentrations of mercuric chloride (Hgcl2) for Explants sterilization and different concentration of NAA) mg/L with 2iP for their effect on initiation primary callus and subsequent embryogenesis in Phoenix dactyliferae cultivar Barhi. Leaf primordial and shoot tips were excised from 2-3 years old offshoot. The result showed that contamination percentage decreased with an increase in concentration of Hgcl2 for two explants where in the control (0.0)% gave highest value 75% and 62.5% of contamination for leaf primordial and shoot tip respectively while the addition (0.1)% gave a significant decrease 37.5 and 0% for explants respectively. The Explants were inoculated onto Murashiege and Skoog

1962 (MS) medium supplemented with 2iP 3mg/L and containing 0

25

50 or 100 mg/L of NAA

the addition (50) mg/L of NAA gave highest value 68.8% in percentage of callus initiation with significant difference from addition 0% gave lowest value (18.8)%

as well Explants of shoot tip gave significant difference was 53.1% while leaf primordial gave 28.1%

Primary callus was transferred to fresh MS medium containing (0

5

10 and 20) mg/L of NAA with 3mg/L 2iP

Results were recorded after 12 weeks. A highest value of embryonic callus induction percentage or fresh weight and dry weight 87.5% or 1.56g and 0.47g for the character respectively for the treat 20 mg/L while control treatment 0.0 gave the lowest value 12.5% or 0.37g and 0.14g for same character respectively. Inoculated 1g fresh biomass into a 250 ml Erlenmeyer flask containing 50 ml MS liquid medium supplemented with NAA 20mg/L and agitated at 150 rpm by shaker to making suspension culture the measurements of packed cell volume ( pcv) or fresh and dry weight taken weekly for 12 weeks

maximum values were reached in the eighth week of PCV and fresh weight but the dry weight reached to highest value in seventh week

then the curve began to decline gradually until it reached stability in the last two weeks. The cell suspension was Diluted to different initial cell density of 100

500

10000

50000 and 100000 cells /ml with hemocytometer. The initial cell number of (10000) cells gave significant different of plating efficiency PE (11.6)% while (100) cells gave lowest value 1.8% of PE.

Ali A.

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View Publication

Publication Date

Mon Apr 01 2019

Journal Name

Biochemical And Cellular Archives

Influence of chemical fertilizers and foliar spraying with humic acid in growth and yield of red cabbage

Mohammed Mahmood

Ridha Mustfa

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(6)

Publication Date

Wed Oct 01 2014

Journal Name

Al–bahith Al–a'alami

Study of the patterns of media frames used in the production of Arabic spring breaks in Al Arabiya channel

Styles

media frames

production breaks

Arab Spring

Al Arabiya Channel

ايمان

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production of the spot in the news channels - a study of patterns of spot media used in the production of spring breaks Arab Al-Arabiya

The spacers news in Arabic, one of the messages that seek channel output are fit and policy on the one hand and meet the needs of viewers for information intensive and image influential to find out the latest developments on the Arab arena, especially the Syrian revolution on the other and thus The viewer for the type of coverage the stomach through a newsletter about the event on the one hand to keep following up on her and eager to watch the other hand

And sou

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