Hepatitis B and Hepatitis C viruses are the major health problem in the worldwide. In the Middle East, the prevalence of HBV in general population with the chronic infectionsis 2-5%,whereas the prevalence of HCV is about 1% in Arabian Gulf countries. World Health Organization (WHO) revealed that the risks of HBV and HCV transmissionas well as human immunodeficiency virus (HIV) through the transfusion of contaminated blood and blood products is high, because of the fragility of health services in these countries. Several viral diseases are transportby different modes like bloodtransfusion, sexual contact, and unsafe injections. The mostcommon blood-transmitted viruses are hepatitis B virus(HBV), hepatitis C virus (HCV) and humanimmunodeficiency virus (HIV);which cause fatal and chronic disorders. The prevalence ofthese viruses varies in Asia and developing countries depending on nationality, geography and lifestyle, sex, race, and educational levels. For all these previous causes, thisstudydesigned to know the seroprevalenceof HBV, HCV and human retroviruses(HIV-1, 2) among inpatients and outpatients of Balad General Hospital in Saladin governorate in Iraq. The study conducted in Balad General Hospital. Subjects with hepatitis C, B viruses, and HIV recorded from January2017 to December 2017. The age of patients ranged from 6 to 70 years; and the total number of tested serum samples was 32,942 serum samples. The highest percentage of HBVprevalence was in July by using ELISA technique (11.1%) for outpatients followed by 15.40% for outpatients in May by using anti-HBV strip.While the highest percentage of HCV prevalence was recorded in July by using ELISA technique (11.73%) for outpatients followed by 14.76% for outpatients in May by using anti-HCV strip. The percentage of patients infected with HBV in both sexes (females and males) was higher than the percentage of patients infected with HCV (1.80%vs.0.57%, respectively). Also, the present study indicated the seroprevalence of HBV (anti-HBV) was 38.27% for female and 41.02% for male with age range 21-30 years, and this significantly higher than in HCV which thepercentage of the seropositive cases were 37.03% in female and 39.13% in male with age range 41-50 years.Therewere no significant differences betweenthe seroprevalences of eachof anti-HCV Ab,anti-HBV Ab and HBsAg,and only 2 cases (0.03%) were seropositive for HIV-1, 2. Although, the HBV and HCV in Iraq are a major problem for the community and viral infectious diseases;the current study revealed that Iraq has a low prevalence for HBsAg, HBV, HCV and HIV.