The Gram-negative bacillus Serratia marcescens (S. marcescens) belongs to the Enterobacteriaceae family. S. marcescens was once thought to be a nonpathogenic saprophytic organism that appeared on decomposing organic matter, animals, plants, and foods, water, and soil. However, it has evolved into an opportunist pathogen that causes nosocomial infections, where it is linked to a variety of hospital-acquired infections, including conjunctivitis, septicemia, wound, and eye infections, pneumonia, meningitis, osteomyelitis, and endocarditis, in addition to respiratory tract and urinary tract infections (UTI). Chitinase, protease, nuclease, lipase, and hemolysin are among the products released by S. marcescens strains, and many of these compounds are probably virulence factors in human infections caused by this disease. Prodigiosin pigment is a natural red produced by S. marcescens which exhibits anticancer, and immunosuppressive properties furthermore to antimicrobial activities. This bacterium has been identified as an opportunistic pathogen that may harbor multidrug resistance mechanisms that complicate treatment, many S. marcescens strains are resistant to a wide range of antibiotics. There are two categories of S. marcescens strains: non-pigmented (white), and pigmented (red). Two stages are involved in the biosynthesis of prodigiosin. The bipyrrole moiety 4-methoxy-2,2-bipyrrole-5-carbaldehyde (MBC) and the monopyrrole moiety 2-methyl-3-n-amyl-pyrrole (MAP) are produced in the first step, which is also referred to as the cytoplasmic step. The condensation of the terminal products of the two parallel routes, MBC and MAP, to generate prodigiosin is the second step in the final biosynthetic process. The aim of this research is to study and know the use of prodigiosin in an applied and medical field and the method of extracting and purifying prodigiosin and the possibility of genetic manipulation of S. marcescens.
In present days, drug resistance is a major emerging problem in the healthcare sector. Novel antibiotics are in considerable need because present effective treatments have repeatedly failed. Antimicrobial peptides are the biologically active secondary metabolites produced by a variety of microorganisms like bacteria, fungi, and algae, which possess surface activity reduction activity along with this they are having antimicrobial, antifungal, and antioxidant antibiofilm activity. Antimicrobial peptides include a wide variety of bioactive compounds such as Bacteriocins, glycolipids, lipopeptides, polysaccharide-protein complexes, phospholipids, fatty acids, and neutral lipids. Bioactive peptides derived from various natural sources like bacte
... Show MoreCephalexin and its derivatives are commonly utilized in the pharmaceutical and medicinal industry due to their biological and pharmaceutical activities, including anti-microbial, anti-cancer, anti-bacterial, and herbicidal activities as well as possessing high palatability and being useful for skin and joint infections. Interestingly, some organic drugs, including cephalexin, which exhibit toxicological and pharmacological properties, can be administered in forms of metal complexes. Many researchers have synthesized organic ligands derived from cephalexin in forms of Schiff bases and azo compounds which exhibited higher biological and medicinal properties when compared to cephalexin alone. One of the important features that make Schiff base
... Show MoreIn this study Microwave and conventional methods have been used to extract and estimate pectin and its degree of esterification from dried grapefruit and orange peels. Acidified solution water with nitric acid in pH (1.5) was used. In conventional method, different temperature degrees for extraction pectin from grape fruit and orange(85 ,90 , 95 and 100?C) for 1 h were used The results showed grapefruit peels contained 12.82, 17.05, 18.47, 15.89% respectively, while the corresponding values were 5.96, 6.74, 7.41 and 8.00 %, respectively in orange peels. In microwave method, times were 90, 100, 110 and 120 seconds. Grapefruit peels contain 13.86, 16.57, 18.69, and 17.87%, respectively, while the corresponding values were of 6.53, 6.68, 7.2
... Show MoreDysmenorrhea is a common menstrual disorder among young women and is frequently associated with physical discomfort, emotional distress, and functional limitations. Among medical students, the demanding academic environment may further exacerbate its impact. However, data from Iraqi medical students remain limited, despite the potential academic and quality-of-life consequences. This study aimed to assess the prevalence of dysmenorrhea, its impact on academic performance and quality of life, and associated coping strategies and health-seeking behaviors among female medical students.
Used cooking oil was undergoing trans-esterification reaction to produce biodiesel fuel. Method of production consisted of pretreatment steps, trans-esterification, separation, washing and drying. Trans-esterification of treated oils was studied at different operation conditions, the methanol to oil mole ratio were 6:1, 8:1, 10:1, and 12:1, at different temperature 30, 40, 50, and 60 º C, reaction time 40, 60, 80, and 120 minutes, amount of catalyst 0.5, 1, 1.5, and 2 wt.% based on oil and mixing speed 400 rpm. The maximum yield of biodiesel was 91.68 wt.% for treated oils obtained by trans-esterification reaction with 10:1 methanol to oil mole ratio, 60 º C reaction temperature, 80 minute reactio
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