Potential pattern of foodborne bacteriophages against multidrug-resistant pathogens was a promising hygienic strategy module. Post-antibiotics era becomes evident due to emerging of dramatic episodes of infectious foci harboring biofilm and multidrug-resistant pathogens transferred mainly throughout food chain. Vancomycin-resistant enterococci (VRE) were struggling among these new infectious emergencies. Phenotypic epigenetic transit tolerant drift cascaded by genetic resistant shift behaviors of recalcitrant VRE forbidden clones recovered from mastitis cases in Cows from territories of Abu-Ghraib, Al- Fudhaliyah and Al-Sadrya in Baghdad ecosystem were combated by redirected lytic bacteriophages cocktails recovered from the same raw-milk ecosystem. Modified and verified techniques were dependent for this enrollment. Plaques-forming techniques (PFU.ml-1) posted by Vega3 scanning electron microscopy confirm recovery and potency of unveiled phages. Frequency and distribution pattern of VRE clones unveiled 27 strains (30%) out of 90-pooled raw-milk samples, as 19 strains (21.11%) of E. faecalis versus 8 strains (8.88%) of E. faecium. Resistance profile index reveal diverse degree of resistance to beta-lactams and vancomycin of all recovered clones. Diverse and intelligent phage profile lytic and bactericidal index was present among recovered VRE clones in which, verified (5-9) logs PFU.ml-1 reduction load noticed according to degree of potency for both recovered clones and phages cocktails. Milieus with new active bio-preservative and matrixed nano biosensor lytic foodborne phages against multidrug-resistant entities of enterococci located and borne in the same niche ecosystem reveal a promising tool for healthy lifestyle in Baghdad.
