Background: Reliable detection the etiological agent of amoebic dysentery and extra-intestinal amoebiasis have Public health importance specially in asymptomatic human and animals, Since the acquisition of pet dogs in the recent period has become widespread in our city. Aim: To give correct perception of infection rate in asymptomatic individuals (human and domestic dogs) for the first aspect and about detection and diagnosis of the pathogenic species of Entamoeba histolytica from another morphologically similar and commensal one using the molecular technique in stool samples of asymptomatic individuals the second aspect. Methods: During the study period from the beginning of September 2020 to the end of February 2021, a total of 95 stool sample was collected from asymptomatic 71 male and 24 female ages between 20-45 years old also 50 fecal samples of asymptomatic domestic dogs (male) belongs to some patients regardless of the breed were in counter. Results: Microscopic and molecular diagnosis for E.histolytica was done using traditional wet mount method and Real Time polymerase chain reaction (RT-PCR) employing phospholipase gene respectively. The result highlighted the microscopic diagnosis of cyst stage in 33 asymptomatic patient out of 95 (34.73%) with significant differences P<0.05 between males 22/71(66.66%) and females 11/24 (33.33%). For domestic dogs, 9 out of 50 samples (18%) were detected as positive. On the other hand the molecular diagnosis results showed presence of E.histolytica in 10 sample out of 33 (30.3%) with non significant differences P>0.05 between males 7/22 (31.81%) and females 3/11 (27.27%) while for domestic dogs no positive results were recorded. Conclusion: The presence of the pathogen E.histolytica in asymptomatic patients. The microscopic examination of stool sample show a possibility of error to differentiate pathogenic E. histolytica from those of morphologically similar non-pathogenic species lead to inaccurate results therefore, the molecular methods must be adopted for diagnosis. Domestic dogs do not pose a risk of transmitting E.histolytica infection but it can transmit other types of pathogens.
The purpose of the search definition on factors superior this whoever impact on the improvement the exploitation decision this detect on stock exchange markets assumption , and using questioners to data accumulate and the Iraq market of financial papers battleground to search annoy chose the eyeball extension (47) peoples from peoples employees on the market , and using the bought statistic program (SPSS) on input and analysis search data , and machinate the search outputs to their group of factors internal and external which impact on exploitation decision on financial papers markets , and the search commend on necessary rise the incumbency direction in the stock exchange
... Show MoreThis study aimed for isolation and identification of Candida glabrata and identifying some virulence factors. The distribution of patients with candidemia thrush showed that the age group 50-65 years old recorded the highest incidence of candidiasis in female and male with leukemia by 50% and 37.9 % respectively compared to the lowest incidence of candidiasis in the age group under 17 years old in female and male 8.8% and 13.5%, respectively. While the age group between 5-8 years was high, reaching 18 cases of oral candidiasis and 42% of children with leukemia compared with the age group, which was the least, that reached 9 cases, 21%. The highest incidence of C. glabrata was 59 isolates of females and males with leukemia, while C. kefyer w
... Show MoreIdentifying phenolic compounds in some genera belonging in the Amaranthaceae family by HPLC technique
Around fifty isolates of Salmonella enterica serovar Typhi were isolated from blood specimens of patients referring to several hospitals in Kirkuk province, Iraq. The results revealed that all isolates developed resistance to trimethoprim-sulfamethoxazole and chloramphenicol. However, neither sul2 nor tem genes were detected. Moreover, only ten isolates were positive for catP. Our data suggested participation of other genes or mechanisms allow these multidrug isolates to resist the antibiotics in question.
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The study aimed to prepare quick response codes to learn some of the technical skills of the second graders in the Faculty of Physical Education and Sports Sciences. The experimental method was used in the design of the experimental and control experimental and control groups. The research sample was represented by second-graders in the College of Physical Education and Sports Sciences / University of Baghdad, and by lot, the second division (a) was chosen to represent the experimental group that applied the inverse method using the QR code, and the second division (g) to represent the control group and applied the traditional method. (10) Students per group. After the tribal tests, his main experiment was carried out for 10 weeks with one
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