Klebsiella pneumoniae has emerged as one of the eight most essential causes of urinary tract infection (UTI), with increasing resistance to antibiotics, especially carbapenems. About one hundred-eight urine samples were collected from inpatients and outpatients who attended. They were collected at three Baghdad hospitals: two from Karkh (AL-Yarmouk Hospital and Al-karamu Hospital) and one from Rusafa (AL-Baghdad Educational Hospital). A minimal inhibitory concentration (MIC) test was done using the Microtiter plate method and demonstrated different resistance levels against meropenem antibiotics. Gene expression of blaOXA-48 and blaVIM-1 genes was performed when treated with meropenem antibiotic using the Real-time PCR technique. The study showed that the highest value of gene expression in the blaOXA-48 gene was recorded for the resistant group was (1.8705), and the lowest value of gene expression in the OXA-48 gene before treated with the meropenem group was (1.0312). The gene expression value in the non-treated group was (0.97) control. In contrast, A slight increase in gene expression value for the blaVIM-1 gene was recorded for the meropenem-treated group (1.8705), and the sensitive group had a gene expression value of (1.00). The lowest value was in the gene expression of the meropenem-treated group (1.0312), as well as an increase in the value of the expression. The genotype of the blaVIM-1gene for the meropenem-treated group (1.828) is based on the 2-ΔCt method for both genes. When using the 2-ΔΔCt method, gene expression differed slightly in both genes. When relying on the 2- ΔΔCt method, both genes' gene expression was slightly different. It was concluded that the coexistence of blaVIM-1 and blaOXA-48 genes in four strains of K. pneumoniae indicated widespread VIM-1 and OXA-48 in Baghdad, Iraq. Keywords: Klebsiella Pneumonia, blaOXA-48,and blaVIM-1, UTI infection
total of 17 Escherichia coli isolates were collected from urine specimens of patients with urinary tract infection. Antibiotics sensitivity test indicated that amikacin followed by chloramphenicol and ciprofloxacin are the most effective antibiotics. The isolates showed multidruge resistant, nine isolates were resistant to 11-15 antibiotics, 3 were resistant to 16-20 antibiotics and 5 were resistant to 21-25 antibiotic. Two isolates were selected, the first (ED1) was resistant to (22) antibiotics while the second isolate (ED2) was resistant to (14) antibiotics (out of 25). Minimum inhibitory concentration (MIC) of the black and green tea water boiled extracts were determined towards (ED1,ED2).Results showed that MIC of black tea extr
... Show MoreThe recent studies suggested the possible toxicities or genetic alterations associated with biological and medical applications of silver nanoparticles (AgNPs). The current research is directed to see if AgNPs administration can lead to some changes in expression of BRAF gene in selected body organs tissues. Fifty-six male of musmusculs (Balb/C) mice from the animal house of Al-Nahrain Centre of Biotechnology were used. These animals were divided randomly to seven groups (eight mouse in each group), one of these groups represented the control group, three groups were subjected to different doses of AgNPs (0.25, 0.5and 1 mg/kg of body weight) for one week, and the remaining three groups were subjected to three different doses of AgNP
... Show MoreBackground: The influence of glucose metabolism is seen in many infectious diseases, making diabetic patients more vulnerable to sepsis and other serious sequelae of bacterial invasion such as UTI and vaginitis.Patients and method: sixty two patients (women) were suffering from GUTIs consulting Al-Elwya hospital from November- 2009 to March -2010. Two samples were taken from those patients (urine samples and vaginal swabs); these samples were cultured on Blood, Chocolate, and MacConkey's agar for isolation of bacteria.Results: The study group consists of sixty two women suffering from (GUTI), their ages range from 18-55 years. Thirty eight of them were diabetic women and twenty four of them were non- diabetic women. Twenty t
... Show MoreThe study included 200 samples were collected from children under two years included (50 samples from each of Cerebrospinal fluid, Blood, Stool and Urine) from, (Central Children Hospital and Children's Protections Educational Hospital) The Iraqi Ministry of Health, the Department of Health Baghdad .the period from the first of 2015 September to the first of December 2015, Were obtained isolates bacterial subjected to the cultural, microscopic and biochemical examination and diagnosed to the species by using vitek2 system .The results showed there were contamination in 6.5% of clinical samples. The diagnosed colonies which gave pink color on the MacConkey agar, golden yellow color on the Trypton Soy agar and green color on t
... Show Moreدرست عملية تحييد البلازميد لسبع عزلات محلية لبكتريا pneumoniae Klebsiella المتعددة المقاومة للمضادات الحيوية والمعزولة من حالات التهابات المجاري البولية باستخدام مادتي الـ Acridin .(SDS) Sodium dodecyl sulphate والـ orange أظهرت النتائج ان العزلات المحلية لجرثومة pneumoniae.K كانت ذات مقاومة عالية جدا ًاذ بلغت (%100) لمضادات امبيسيلين،ببراسيلين، اموكسيسيلين، بنسلين ج، تتراسايكلين، ارثرومايسين وجنتامايسين واظهرت مقاومة عالية (71–%86) لمضادات
... Show MoreMicrosporum canis is considered one of the filamentous fungi that cause surface fungal contagion in the humans and animals. The present study aimed to diagnose M. canis via the molecular method and differentiating its local Iraqi isolates from global isolates. Microscopic examination showed 55 specimens with M. canis from 130 specimens collected from children aged between 4-10 years suspected of dermatophytes who attended Medical City Laboratories and Baghdad Hospital in Baghdad city from 1/12/2022 to 1/3/2023. The results showed that the frequency of M. canis infections was 55/130 (42.31%). The results demonstrated significant differences in the animals' contact (p <0.0001), lesions (0.03) and habitation area (p =0.002). Whilst
... Show MorePseudomonas aeruginosa is a common and major opportunistic human pathogen, its causes many and dangersinfectious diseases due to death in some timesex: cystic fibrosis , wounds inflammation , burns inflammation , urinary tract infection , other many infections otitis external , Endocarditis , nosocomial infection and also causes other blood infections (Bacteremia). thereforebecomes founding fast and exact identification of P. aeruginosafrom samples culture very important.However, identification of this species may be problematic due to the marked phenotypic variabilitydemonstrated by samples isolates and the presence of other closely related species. To facilitate species identification, we used 16S ribosomal DNA(rRNA) sequence data
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