The present work focuses on the experimental implementation of one of the fiber optical sensors, the optical glass fiber built on surface Plasmon resonance. A type of optical glass fiber was used in this work, single-mode no-core fiber with pre-tapering diameter: (125.1 μm) and (125.3 μm), respectively. The taper method can be tested by measuring the output power of the optical fiber before and after chemical etching to show the difference in cladding diameter due to the effect of hydrofluoric acid with increasing time for the taper process. The optical glass fiber sensor can be fabricated using the taper method to reduce the cladding diameter of the fibers to (83.12 µm, 64.37 µm, and 52.45 µm) for single-mode fibers using Hydrofluoric acid to enhance its properties. Next, SPR-based glass fibers were used as a biomedical sensor to sense and determine the refractive index and hemoglobin concentration in blood samples. The response surface plasmon resonance curve of different blood samples was registered in this study showed a decline in the resonance location. The alteration in the refractive index of the sensing medium changed the wavelength of the response surface plasmon resonance curve.
