Osteoporosis (OP) is asymptomatic disease until the experience of falls or impacts that cause broken bones or fractures happened. Estrogen deficiency, abnormality in bone matrix components and interleukins expression can impact on bone homeostasis which gradually paved to osteoclastogenesis over activation leading to bone loss. A current case-control study was designed to explain the alteration in certain biochemicals, bone matrix components, immunological and molecular parameters that which accompanied with OP in the postmenopausal women. The period of study's investigation was from December 2022 to July 2023. All participants provided written informed consent, and the study was approved by Department of Biology, College of Science, University of Baghdad Ethical Council (Reference code CSEC/0922/0092, on 26-9-2022). One hundred and ten postmenopausal women were randomly chosen to participate in this study based on specific criteria from Al-Wasity Hospital, Medical City and Al- Imamein Kadhimein Medical City Hospitles, Baghdad, Iraq. The age of the participants was ranged from 45 to 70 years. Firstly, Dual Energy X-ray Absorptiometry (DEXA) scan was performed to all participants in order to assess the precence or abscense of OP disease. Out of 110 samples, 70 samples were confirmed with OP. Meanwhile, 40 samples were control. Venous blood samples were collected from all participants to estimated compelet blood count (CBC), immunological parameters as C-Reactive Protein (CRP), Rheumatoid Facter (RF), Anticyclical Citrullinated Peptide Antibody (ACPA), Interleukin-8 (IL8), Interleukin (IL-17), and Interleukins-22 (IL-22) using enzyme link immunosorbent assay (ELISA) technique, Parathyroid Hormone (PTH), and Calicum (Ca+2), bone matrix component parameters osteopontin (OPN), osteocalicn (OCN), and osteonectin (ON), in addition to,Osteopontin gene SNP rs11730582 polymorphism was determined by the Hight Resolution Melting (HRM) analysis. Highly significant (P≤0.01) decrease in WBC count (6.30±0.18 k/mcL) and HGB (9.900±0.132 g/dL) were observed in OP patients as when compared with the control group, WBC was (8.26±0.27k/mcL) and HGB was (13.321±0.287 g/dL), Conversely, highly significant (P≤0.01) increase was recorded in ESR (43.73±1.76 mm/hr), PLT (437.88±7.00 1083/uL) in OP patients in compare with control, (21.10±1.25mm/hr), and (226.79±9.39 1083/uL), respectively. However, highly significant (P≤0.01) increase was noticed in immunological parameters: CRP was (18.26±1.33 pg/ml), RF (IU/ml) results were positive, and ACPA was (23.26±2.74EU/ml) in OP patients when compared with control, CRP (2.92±0.18 pg/ml), RF (IU/ml) results were negative, and ACPA was (6.26±0.56 EU/ml). Beside to, highly significant (P≤0.01) increase was recorded in IL-8 serum level (249.08 ±19.98ng/ml), IL-17 (107.862±9.25ng/ml), and IL-22 (57.73±2.47 ng/ml) in OP patients as compared with the healthy control group were IL-8 (65.29 ± 5.62 ng/ml), IL-17 (46.135 ± 8.66 ng/ml) and IL-22 (13.57 ± 3.16 ng/ml). The mean PTH serum level of OP patients group was (77.80±1.79pg/mL) and in control group was (35.81±2.06pg/mL), whereas a highly significant (P≤0.01) decrease in mean of Ca⁺² serum level was recorded in OP patients (4.89±0.21 mmol/L) in compared with control group (9.63±0.13 mmol/L). Additionally, high significant (P≤0.01) increase was detected in OPN serum level (16.88±0.77 ng/ml) and OCN (29.86±2.25ng/ml) in OP patients as compared with the healthy control group (5.95±0.41ng/ml and 10.73±0.86ng/ml), respectively. In contrast, ON serum level recorded a highly significant (P≤0.01) decrease in the patient group (1.78±0.971ng/ml) compared with the control group (3.42±0.20ng/ml). The frequency of homozygote TT genotype in patient's analysis was, (14.3%), (10/70) than control group (27.5%), (11/40). While, the frequency heterozygote TC and homozygote mutant CC genotypes were [TC= (71.42%) 50/70 and CC= 14.3% (10/70)] and these genotypes frequency were not significantly differet from those of the control group, (TC= 60%, (24/40), and (CC= 12.5% 5/40). P-value was different (0.0158) significantly in genotype distribution of rs11730582 polymorphism in OP patients whereas, the P-value of rs11730582 was (0.1500) non-significant in control group. In conclusion, this study found that OPN, OCN, and ON protein act as a sensitive monitoring indicator can be used in early detection of osteoporosis. And because of the capacity to activate osteoclastogenesis and promote bone resorption, IL-8, IL-17, IL-22 and rs11730582 of OPN gene polymorphism may be serve as a biomarkers, pro-inflammatory and immune-stimulating factors in OP development.
It was aimed to understand the interleukin-4 (IL-4) role in etio-pathogenesis of rheumatoid arthritis (RA). Two approaches were adopted. In the first one, a quantitative expression of IL4 gene was assessed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and such findings were correlated with some demographic, clinical and laboratory parameters, which included gender, duration of disease, disease activity score (DAS-28), rheumatoid factors (RFs), C-reactive protein (CRP) and anti-cyclic citrullinated peptide (ACCP) antibodies. In the second approach, a single nucleotide polymorphism (SNP) of IL4 gene (rs2243250) was inspected by DNA sequencing using specific primers. Fifty-one Iraqi RA patients (22 males and 29 fem
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ABSTRACT
The study was conducted at the ruminant research station of the general commission for agricultural research/Ministry of Agriculture, as well as the laboratory of genetic resources of the department of livestock/Ministry of Agriculture and the laboratory of the college of agriculture engineering science, with the aim of determine the genotypic of the expression region (intron 2 and part of exon 3) of the LHX3 gene And its relationship to the fertility rate in local and Shami goats. For this purpose, the RFLP technique was used, and the percentages of genotypes for the LHX3 gene in the local goat sample were 29.17, 50.00, 20.83 for the TT, AT, and AA genotypes, respectively, while in the Shami goa
... Show MoreNeuron-derived neurotrophic factor [NENF], a human plasma neurotrophic factor, also increases neurotrophic activity in conjunction with Parkinson's disease-related proteins in Neudesin. Although Neudesin (neuron-derived neurotrophic secreted protein) is a member of the membrane-associated progesterone receptor (MAPR) protein subclass, it is not evolutionary related to the other members of the same family. The expression of Neudesin is found in both brain and spinal cord from embryonic stages to adulthood, as w Neudesin levels in Parkinson's patients with osteoporosis disease and Parkinson's patients without osteoporosis disease, as well as the relationship between Neudesin levels, Anthropometric and Clinical Features (Age, Gender, BMI) and
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Osteoporosis is a systemic disease of the skeleton, characterized by low bone mass and alteration in the micro-architecture of the bone tissue that lead to an increase in brittleness with the ensuing predisposition to bone fracture. Global statistics shows that women are more exposed to this disease than men and in particular at menopause. This study was designed to evaluate the use of some bone markers: serum osteocalcin (Ost), alkaline phosphatase (ALP), as bone formation markers, also parathyroid hormone (PTH), calcium and inorganic phosphate level, for the assessment of patients with osteoporosis and to evaluate their role in monitoring of several types of therapeutic interventions (such as bisphosphonates, hormonal replacement thera
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Ankylosing spondylitis (AS) represents one kind of advanced arthritis formed via inflammatory stimuli long-term in the spin‘s joints. Interleukin (IL)-29 (interferon- lambda1(IFN- λ1)), interleukin (IL)-28A (interferon- lambda 2 (IFN- λ2)) and interleukin (IL)-28B (interferon- lambda 3(IFN-λ3)) are three interferon lambda (IFN- λs) molecules that have recently been identified as new members of the IFN family. IL-28B expression in ankylosing spondylitis (AS) is not well understood. 150 male healthy controls ((HC) and 160 males with AS as patients group participated in this study. Serum level and gene polymorphism were assessed using an enzyme-linked immunosorbent assay and Sanger sequencing for IL-28B, respectively. The results showed
... Show MoreKidney disease is a kidney injury or disease that affects many people globally. The study aims to evaluate the effect of Phosphatidyl Inositol (4,5) Bisphosphate, 5-Phosphatase (PIP2) enzyme on calcium (Ca2+) levels and its relationship to oculocerebrorenal gene variations in kidney stone and kidney failure patients. The enzyme-produced amount was measured by enzyme-linked immunosorbent assay (ELISA). Genetic variations were studied through the polymerase chain reaction (PCR) technique, followed by the sequencing of fragments of the exons (9, 13, 15). Blood samples were collected from eighty patients (40 with kidney stones and 40 with kidney failure), and 40 were healthy individuals. Results showed a significant difference between t
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Background: All diseases concerning bone destruction such as osteoporosis and periodontal diseases share common pattern in which the osteoclast cells are absolutely responsible for bone resorption that occurred when osteoclast activity exceeds osteoblast activity. Osteoprotegrin (OPG) considered as novel soluble decoy receptor known as “bone protector†since it prevents extreme bone resorption through inhibition of differentiation and activity of osteoclast by competing for binding site. It binds to receptor activator of nuclear factor kappa-B ligand (RANKL) and prevent its interaction with receptor activator of nuclear factor kappa-B (RANK), thus inhibits osteoclast formation. TNF-α is a pro-inflammatory cytokines having
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