In Vitro Cytotoxic Effect of Aqueous Extract of Origanum Marjoram on AMN-3 Cell Line
Publication Date
Fri Mar 27 2020
Journal Name
Plant Archives 20 (supplement 1), 1666-1670
Publication Date
Mon Apr 06 2020
Journal Name
August
INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION
AND GERMINATION OF DATE PALM CV BARHI BY USING CELL
SUSPENSION CULTURE TECHNIQUE
This study was done to examine different concentrations of mercuric chloride (Hgcl2) for Explants sterilization and different
concentration of NAA) mg/L with 2iP for their effect on initiation primary callus and subsequent embryogenesis in Phoenix
dactyliferae cultivar Barhi. Leaf primordial and shoot tips were excised from 2-3 years old offshoot. The result showed that
contamination percentage decreased with an increase in concentration of Hgcl2 for two explants where in the control (0.0)%
gave highest value 75% and 62.5% of contamination for leaf primordial and shoot tip respectively while the addition (0.1)%
gave a significant decrease 37.5 and 0% for explants respectively. The Explants were inoculated onto Murashiege and Skoog
1962 (MS) medium supplemented with 2iP 3mg/L and containing 0
25
50 or 100 mg/L of NAA
the addition (50) mg/L of NAA
gave highest value 68.8% in percentage of callus initiation with significant difference from addition 0% gave lowest value
(18.8)%
as well Explants of shoot tip gave significant difference was 53.1% while leaf primordial gave 28.1%
Primary callus
was transferred to fresh MS medium containing (0
5
10 and 20) mg/L of NAA with 3mg/L 2iP
Results were recorded after 12
weeks. A highest value of embryonic callus induction percentage or fresh weight and dry weight 87.5% or 1.56g and 0.47g for
the character respectively for the treat 20 mg/L while control treatment 0.0 gave the lowest value 12.5% or 0.37g and 0.14g for
same character respectively. Inoculated 1g fresh biomass into a 250 ml Erlenmeyer flask containing 50 ml MS liquid medium
supplemented with NAA 20mg/L and agitated at 150 rpm by shaker to making suspension culture the measurements of
packed cell volume ( pcv) or fresh and dry weight taken weekly for 12 weeks
maximum values were reached in the eighth week
of PCV and fresh weight but the dry weight reached to highest value in seventh week
then the curve began to decline
gradually until it reached stability in the last two weeks. The cell suspension was Diluted to different initial cell density of 100
500
10000
50000 and 100000 cells /ml with hemocytometer. The initial cell number of (10000) cells gave significant different
of plating efficiency PE (11.6)% while (100) cells gave lowest value 1.8% of PE.
Publication Date
Sun Jan 01 2023
Journal Name
Plant Archives
INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION AND GERMINATION OF DATE PALM CV BARHI BY USING CELL SUSPENSION CULTURE TECHNIQUEe
INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION AND GERMINATION OF DATE PALM CV BARHI BY USING CELL SUSPENSION CULTURE TECHNIQUEe
Publication Date
Wed Apr 01 2020
Journal Name
Indian Journal Of Forensic Medicine And Toxicology
(2)
Publication Date
Thu Jan 01 2026
Journal Name
Journal Of Nanostructures
Cytotoxic and Antibacterial Activity of Yttrium Oxide Nanoparticle Y2O3 Against Serratia Fonticuli and Citrobacter Kasseri Isolated fFrom Cholangitis Patients
Bacterium Cit koseri Cholangitis Neurotoxicity Serratia fonticuli Yttrium oxide nanoparticles
The rise of antibiotic-resistant bacteria necessitates the exploration of novel antimicrobial agents. Yttrium oxide nanoparticles (Y₂O₃) have shown potential due to their unique physicochemical properties and antibacterial activities against various pathogens. This study investigates the cytotoxic and antibacterial effects of Y₂O₃ nanoparticles against Serratia fonticuli and Citrobacter koseri, bacteria isolated from cholangitis patients. Bacterial strains were isolated from bile specimens and confirmed using standard microbiological techniques. The methods of X-ray diffraction (XRD), (SEM), and Frequency transform-infrared spectroscopic (FT-IR) were used to characterize YO₃ particles. Using a microdilution technique, the minimum
... Show MorePublication Date
Sun Jan 01 2023
Journal Name
Materials Today: Proceedings
(24)
(17)
Publication Date
Sun Jan 01 2023
Journal Name
Materials Today: Proceedings
(24)
(17)
Publication Date
Thu Dec 31 2015
Journal Name
Iraqi Journal Of Chemical And Petroleum Engineering
Effect of Particle Size of Sawdust on Behaviour of Sawdust/UPE Composites in Water
sawdust
unsaturated polyester
hardness
absorbance of water.
The aim of the present work is to develop a new class of natural fillers based polymer composites with sawdust (S.D) which used two particle sizes (1.2 μm & 2.3 μm) and different weight percentage from sawdust (10%, 15%, and 20%). The mechanical properties studied include hardness (shore D) for all samples at normal conditions (N.C). The unsaturated polyester (UPE) and its composites samples were immersed in water for 30 days to find the effect of particle size of sawdust (S.D) on the weight gain (Mt %) by water for all the samples, also to find the effect of water on their hardness. The results show that the composite materials of sawdust (S.D) fillers which has particle size (1.2 μm) better than (2.3 μm) particle size bef
... Show MorePublication Date
Thu Jun 18 2015
Journal Name
Journal Of Kerbala University
Effect of Carbamazepine drug on formation of decidual tissue in femal of Mus musculus
Implantation of blastocyst is considered as a sensitive process in the life of growing embryo. Thus this critical period could be under threat from the harmful effect of many drugs ,among them is the carbamazepine which is used by many women for a treatment of epilepsy . The present experiment was designed to study the effect of this drug on the process of implantation ;especially during the days 7 and 9 of pregnancy. For those purpose 169 pregnant albino mice, of 25 gm weight for each one, were used .The mice were divided into control group given orally normal saline, and treated group given orally carbamazepine with concentration of 15 mg\kg. Samples from pregnant uteri containing the embryos were used. They were first fixed in Boun's sol
... Show MorePublication Date
Wed Feb 01 2023
Journal Name
Baghdad Science Journal
Evaluation of Beta-2 Integrin and Platelets Roles in Sickle Cell Disease Pathogenicity in Basrah Governorate Patients
β integrin(CD18)
pathogenicity
Platelets
Sickle cell disease
Sickle cell disease (SCD) comprises an inherited blood disorder that is life long and affects many people globally. In spite of the development in treatment, SCA is a considerable cause of mortality and morbidity. The present study tries to assess the role of leukocytes represented by β integrin(CD18) and platelets and their productivity in the pathogenicity of disease during the steady state and crisis in comparison with the healthy as-control group, SCD patients (15) enrolled during crisis and steady state (follow up) showed a significant increase in leukocytes and platelets cells productivity during crisis when compared to the steady state and in the steady state when compared to the healthy control group . In this study, SCD patho
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(2)
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