The present study aimed to investigate the histology of the testis in golden hamster during 1, 28 and 60 day of old. Tissue samples were processed, sectioned and subjected to specific stains to reveal histological details. Observation of one day old showed the testicle enclosed by undifferentiated thin layer of testicle capsule which consisted of undifferentiated myoblasts and fibroblasts small size seminiferous tubules appeared, one layer of spermatogonium cells (Type-A) which revealed mitotic figures, at 28 day old the capsule was consisted of thick outer fibromuscular layer and thick inner vascular layer that revealed encouraged blood vessels and cells, Spermatogonial cells type-A & B appeared as large size with darkly stained nucleus and eosinophilic cytoplasm,as well as Primary and secondary spermatocytes appeared slightly small size cells comprised of small lightly stained nucleus at 60 day parenchyma was buildup by enlarged size seminiferous tubules that revealed complete differentiated spermatogenic cells and each tubule was surrounded by thin fibrocellular membrane with myoid cells. Spermatogonial cells types-A and B appeared enlarge size with darkly stained nucleus and eosinophilic cytoplasm, as well as Primary and secondary spermatocytes appeared slightly smaller in size cells, comprised of small lightly stained nucleus.
