Urinary tract infection is a bacterial infection that often affects the bladder and thus the urinary system. E. coli is one of the leading uropathogenic bacteria that cause urinary tract infections. Uropathogenic E. coli is highly effective and successful in causing urinary tract infections through biofilm formation and urothelial cell invasion mechanisms. Other organisms that cause urinary tract infections include members of the Enterobacteriaceae family, streptococci and staphylococci species and perch. In addition, K.penumoniae is another important gram-negative bacterium that causes urinary tract infections. With the PCR technique, unseen bacterial species can be detected using standard clinical microbiology methods. In this study, the antibiotic resistance of E. coli and K. penumoniae bacteria causing urinary tract infection was analyzed by PCR technique. As a result of the experiments conducted within the scope of our study, it was found that bla SHV, one of the virulence factors of E. coli isolates, and bla CTX-M, one of the genes that produce ESBL, were related that both these virulence factors can be found at the same time in ESBL positive and negative isolates. It appeared that bla CTX-M gene is not detected in any of the ESBL negative isolates. It demonstrated that the bla CTX-M gene was more dominant in the development of resistance to β-lactam group antibiotics. Also, the results of the experiments conducted within the scope of our study, the frequency percentage of β-lactamase resistance genes (bla TEM, bla SHV and bla CTX-M) increased in K. pneumoniae compared to E. coli isolates. Moreover, phenotypic and genotypic methods are needed to detect the presence of different gene products associated with resistance in E. coli and K. pneumoniae isolates.