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Effects of Avena sativa and Glycyrrhiza glabra Leaves Extracts on Immune Responses in Serum Cytokine and Liver Enzyme Levels in NIH Mice
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In addition to their high quantities of active chemicals, medicinal plants are well-known for their pharmacological qualities, which include immunological modulation. T Consequently, this study aimed to examine the effects of Avena sativa and Glycyrrhiza glabra leaf extracts on immunological responses as measured by blood cytokine and liver enzyme levels. The phytochemical analysis of Avena sativa crude leaf extracts revealed the presence of alkaloids,flavonoids, tannins, phenolic compounds, and saponins but the absence of resins and violet oils. On the other hand, violet oils, flavonoids, tannins, saponins, and glycosides were detected in significant concentration in Glycyrrhiza glabra ethanolic extract, although resins and phenolic compounds were not present. Fifty male NIH mice were randomly divided into five groups: Except for the control group, all animals were given subcutaneously and orally with extracts (50 mg/kg) for 14 days prior to LPS-induced (1 mg/kg body weight) liver injury. LPS-induced liver damage was induced on day 15, and mice were starved.Group 1 was injected subcutaneously with normal saline as a control. Group 2 received an injection of 100 l of crude oat extract subcutaneously. Group 3 was administered 100 l (50 mg/kg) of crude Oat extract orally. Group 4: administered 100 l (50 mg/kg) of crude Licorice extract subcutaneously. Group 5 ingested 100 l (50 mg/kg) of crude Licorice extract orally. IL-4 levels were significantly elevated (P 0.05) in the subcutaneously and orally treated groups compared to the control group (12.3 0.23 pg/ml). IL-6 was significantly elevated (P<0.05) in mice given subcutaneously or orally with Avena sativa or Glycyrrhiza glabra extracts compared to mice treated subcutaneously or orally with a control substance (44 0.57 pg/ml). The concentration of TNF- was significantly elevated (P<0.05) in subcutaneous and oral treated groups (283.6 1.7 and 280.6 12.2; 233.9 0.6 and 241.2 2.8) compared with the control group (130 0.42) pg/ml. When mice were exposed to LPS-containing extracts, both GOT, and GPT levels fell relative to the control group.

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Publication Date
Wed Oct 31 2012
Journal Name
Enzyme Research
Simulation of Enzyme Catalysis in Calcium Alginate Beads
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A general mathematical model for a fixed bed immobilized enzyme reactor was developed to simulate the process of diffusion and reaction inside the biocatalyst particle. The modeling and simulation of starch hydrolysis using immobilized α-amylase were used as a model for this study. Corn starch hydrolysis was carried out at a constant pH of 5.5 and temperature of . The substrate flow rate was ranging from 0.2 to 5.0 mL/min, substrate initial concentrations 1 to 100 g/L. α-amylase was immobilized on to calcium alginate hydrogel beads of 2 mm average diameter. In this work Michaelis-Menten kinetics have been considered. The effect of substrate flow rate (i.e., residence time

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Publication Date
Sun Feb 21 2021
Journal Name
Iraqi Journal Of Science
The antimicrobial effects of alcoholic leaves extract of salvia officinalis against multidrug resistant pseudomonas aeruginosa
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Publication Date
Sat Mar 04 2017
Journal Name
Baghdad Science Journal
Measurement and purification of Alanine aminotransferase (ALT) enzyme activity in patients with celiac disease
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Celiac disease (CD) is the most common genetically - based disease in correlation with food intolerance. The aim of this study is to measure the activity of ALT enzyme and purify enzyme from sera women with celiac disease. Alanine aminotransferase (ALT) activity has been assayed in (30) women serum samples with celiac disease, age range between (20-40) year and (30) serum of healthy women as control group, age range between (22-38) year. In the present study, the mean value of ALT activity was significantly higher in patients with celiac disease than healthy group (p<0.01). The ALT enzyme was partial purified from sera women with celiac disease by dialysis, gel filtration using Sephadex G- 50 and ion exchange chromatography using DEAE- cell

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Publication Date
Sun Sep 03 2017
Journal Name
Baghdad Science Journal
Measurement and purification of Alanine aminotransferase (ALT) enzyme activity in patients with celiac disease
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Celiac disease (CD) is the most common genetically - based disease in correlation with food intolerance. The aim of this study is to measure the activity of ALT enzyme and purify enzyme from sera women with celiac disease. Alanine aminotransferase (ALT) activity has been assayed in (30) women serum samples with celiac disease, age range between (20-40) year and (30) serum of healthy women as control group, age range between (22-38) year. In the present study, the mean value of ALT activity was significantly higher in patients with celiac disease than healthy group (p<0.01). The ALT enzyme was partial purified from sera women with celiac disease by dialysis, gel filtration using Sephadex G- 50 and ion exchange chr

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Publication Date
Mon Jan 01 2024
Journal Name
Baghdad Science Journal
Effect of nanocapsules and extract of Metarhizium anisopliae in inhibiting acetylcholine esterase enzyme in Musca domestica larvae.
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M. domestica is the most important insect that transmit pathogens for diseases in the world. The use of nanotechnology is eco-friendly method in control pests. The study aims to investigate the feasibility of bio-manufacturing nanocapsules of fungal secondary metabolites in order to improve the efficiency of metabolite and assess their inhibitory effect on the acetylcholine esterase enzyme in housefly larvae. An equal mixture of organic solvents, ethyl acetate and dichloromethane, was used to extract the metabolic products of the fungus M. anisopliae, (PEG4000) and chitosan was used in the preparation of nanocapsules. The results of the DLS granular size assay showed that the size of the extract particles and the size of the chitosan and

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Publication Date
Sat May 20 2023
Journal Name
Baghdad Science Journal
Effect of nanocapsules and extract of Metarhizium anisopliae in inhibiting acetylcholine esterase enzyme in Musca domestica larvae.
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M. domestica is the most important insect that transmit pathogens for diseases in the world. The use of nanotechnology is eco-friendly method in control pests. The study aims to investigate the feasibility of bio-manufacturing nanocapsules of fungal secondary metabolites in order to improve the efficiency of metabolite and assess their inhibitory effect on the acetylcholine esterase enzyme in housefly larvae. An equal mixture of organic solvents, ethyl acetate and dichloromethane, was used to extract the metabolic products of the fungus M. anisopliae, (PEG4000) and chitosan was used in the preparation of nanocapsules. The results of the DLS granular size assay showed that the size of the extract particles and the size of the chitosa

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Publication Date
Sun Mar 02 2008
Journal Name
Baghdad Science Journal
Colonization of Staphylococcus xylosus in the kidneys and bladder of mice
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Two isolates of Staphylococcus xylosus (urease producer and non urease producer) were injected in mice at a dose of 2 × 108 colony-forming units (CFU) intraurethrally. Results showed that both isolates were able to colonize kidney and bladder of the injected mice, regardless of their urease production. Moreover, there were insignificant differences between the two groups. These results emphasized the pathogenicity of this bacteria in UTI.

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Publication Date
Tue Mar 27 2018
Journal Name
Mustansiria Dental Journal
Topical Nigella Sativa in the treatment of oro-genital ulceration in Behcet's disease.
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To assess the effect of Nigella sativa oil as a topical preparation for treatment of oro- genital ulceration of Behcet's disease. This is a double blinded therapeutic trial in which 40 patients with Behcet's disease manifested as recurrent episodes of oral and genital ulceration were enrolled into 2 groups; Nigella sativa oil was applied as 10% in glycerin topically 3 times / daily to the first 20 patients. The other 20 were given glycerin only. Assessment was done 4 days then 8 days after treatment.Results were recorded as they appeared in clinical examination and those records were arranged in tables for both groups concerning oral and genital ulceration cases.Nigella sativa oil is a new topical agent for promotion of healing of o

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Publication Date
Sun Dec 01 2019
Journal Name
Baghdad Science Journal
Histological and Physiological Studies on the Long-term Effect of Different Concentrations of Energy Drink (Tiger) on the Renal and Hepatic Systems of Young Mice
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The present study aims to investigate the long-term histopathological, and physiological effects of different concentrations of a commercially available energy drink (Tiger) on liver and kidney of young mice. Sixteen Balb/c male mice,6 -week old, were divided into 4 groups (n=4). Two groups consumed the energy drink at a concentration of 28µl energy drink/ml water. One group were killed after 10 days (T1), another group were killed after 20 days (T2). Other group of mice consumed the energy drink at a final concentration of 14µl/ml for 20 days (T3). The last group was provided only with water and served as control. Mice of all groups drank around 3 ml per day. The histopathological study on liver of treated groups showed many changes s

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Publication Date
Sun Jun 01 2014
Journal Name
Baghdad Science Journal
Complete Blood Count and Cinnamic acid activity against Cytoxan in albino mice.
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The aim of study to evaluated cinnamic acid and its activity on complete blood count(RBC,WBC,HG,HCV,MCH,MCHC and Plat.)and removed the cytoxan damage which caused bone marrow failure and leukemia and other that due to linked the cytoxan in 7- nitrogen of guanine based of DNA that lead to dead cells. Two concentration from pure cinnamic acid (5.6, 2.8 mg ? mice weight) in first step to choice the perfect concentration in comparison with each negative control ,positive control of cytoxan and the comparison group represent vitamin C. The second step to understand cinnamic acid mechanism activity towards cytoxan by used pre- cytoxan and post – cytoxan in interaction with perfect concentration of cinnamic acid dose (2.8 mg ? mice we

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