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GENOTYPING OF QUORUM SENSING AND BIOFILM VIRULENCE FACTORS GENES IN LOCAL ISOLATES OF ACINETOBACTER BAUMANNII
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Objective: The current investigation focused on Acinetobacter baumannii (A. baumanni), due to its growing significance as a hospital infection-causing pathogen and its resistance to several medications.Material and Method: Sixty-five isolates of A. baumannii were isolated from wound samples of patients admitted to different hospitals in Baghdad between January and April of 2023. Two types of methods were used in the detection of biofilm formation: the first one was Congo red agar method and the second one was microtiter plate method. Genotypic detection of various virulence factors associated with A. baumannii was performed using monoplex, multiplex, and ERIC-PCR.Result and Discussion: To use the PCR method to examine virulence genes like biofilm and quorum sensing (QS). The genes responsible for biofilm formation were identified by the PCR method, followed by ompA 63/65 (96.92%) and bap 48/65 (73.84%), whereas the genes responsible for chemical signals were found to be rhlI 43/65 (66.15%), LasI 58/65 (89.23%), LasR 56/65 (86.15%), and rhlR was 39/65 (60%) after quorum sensing (QS) system genes. ERIC-DNA fingerprinting's phylogenetic analysis illustrated the variety of all isolates by utilizing the Dice coefficient and the UPGM of phylogenetic analysis. Based on statistical analysis, the ERIC-PCR genotyping method correlation coefficient with the study virulence genes, antibiotic sensitivity test, and other variables of virulence was significant at p <0.05.

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Publication Date
Wed Apr 01 2020
Journal Name
Plant Archives
Determination of heavy metals in tattoo inks from the local market in Baghdad city
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Publication Date
Wed Jun 14 2023
Journal Name
Al-academy
The Effect of a Training Program in Introducing Saudi Traditional Fashion in Local Museums
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Museum education is of great importance to an appropriate representation of museums’ collections and exhibits, including traditional fashion. Therefore, museum educators/curators need to be equipped with the most essential skills in their profession in order to adequately present the museum’s history and holdings. This could be achieved through specialized training programs. However, Arab countries are still behind in terms of museum education. Therefore, this article aims to shed light on this issue by assessing the knowledge and skills possessed by museum educators/curators and how training programs could affect them

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Publication Date
Mon Dec 31 2018
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
STUDYING THE EFFECT OF Proteinase K AND α-Amylase ON INHIBITION OF Staphylococcus aureus AND Klebsiella pneumoniae BIOFILM ISOLATE FROM URINARY TRACT INFECTION.: STUDYING THE EFFECT OF Proteinase K AND α-Amylase ON INHIBITION OF Staphylococcus aureus AND Klebsiella pneumoniae BIOFILM ISOLATE FROM URINARY TRACT INFECTION.
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The current study aimed to investigate the viability of biofilm formation klebsilla pneumoniae and Staphylococcus aureus. 440 urine samples were collected from patients suffering from urinary tract infection (UTI) from those who were admitted and visitors to Al-Ramadi Teaching Hospital, Al-Yarmouk Teaching Hospital, Al-Ramadi Teaching Hospital for women and children and , Teaching Laboratories in the Medical City for both genders for a period extended from 5 July, 2017 to 10 October, 2017. Samples were diagnosed by culturing them on a selective media and by biochemical testes , also, diagnosis was ensured by using VITEK-2 compact system. Results showed that K.pneumoniae isolation ratio was 17.1%(68) and S.aureus ratio was 13.1%(52). Thei

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Publication Date
Tue Jan 01 2019
Journal Name
Plant Archives
Spatial distribution of some fertility elements in some northern Iraqi soils using geomatic techniques (remote sensing)
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Publication Date
Thu Apr 07 2016
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
DETECTION OF MICROBIAL CONTAMINATION IN SOME TYPES OF FLOUR THAT AVAILABLE IN LOCAL MARKETS USING BACTRAC DEVICE 3400: DETECTION OF MICROBIAL CONTAMINATION IN SOME TYPES OF FLOUR THAT AVAILABLE IN LOCAL MARKETS USING BACTRAC DEVICE 3400
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A microbial study conducted for a number of flour samples (30 samples) Uses in the bakery ovens in various areas of the city of Baghdad, by used the conventional methods used in laboratories in microbial tests and compared with the modern techniqueby usedof BacTrac Device 3400 equipped from SY-LAB Impedance analysersAustrian company.The results of two ways showed (The conventional way and BacTrac Device test)that the total counts of aerobic bacteria, coliform bacteria, StaphylococcusSpp. bacteria, Bacillus cereus bacteria and yeasts and molds,Most of them were within the permissible borders in the Iraqi standard for grain and its products With free samples from SalmonellaSpp. bacteria, and that the screening by BacTrac device are shorten

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Publication Date
Sat Jan 01 2022
Journal Name
Archives Of Civil Engineeringthis Link Is Disabled
Factors affecting time and cost trade-off in multiple construction projects
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Publication Date
Thu Dec 31 2020
Journal Name
Journal Of Accounting And Financial Studies ( Jafs )
Creative accounting and the factors affecting its practice in Iraqi companies
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The research aims at introducing accountability creative and the factors influencing or helping to Do in Iraqi companies, was launched two assumptions first that he was effect with significance to a number of factors in the direction of departments in Iraqi companies to practice creative accounting , were selected (9) factors believed to researchers it's most influential, and second that there importance of significance for a number of ways or methods to prevent or combat creative accounting , and the purpose of testing hypotheses have been designed questionnaire was distributed to a sample of 48 individual practitioners accountable in Iraqi companies and auditors in charge of auditing the accounts of those companies , after anal

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Publication Date
Sun Apr 03 2011
Journal Name
Journal Of Educational And Psychological Researches
Satisfy of life and it's relationship; with the bige factors of character in the university student
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This research aim to recognize
satisfy of life in the university's student and balance in satisfy of life
according to two type aveirable sex specific and find type and
relationship's director between satisfy of life and character big factors.
and known hom much that Factors in satisfy of life, the research sample
consist of (401) student male and female and from both specified in
Baghdad's University, researcher bult scale of Costa &McCrae Fore the
fire big Factors to Personality, research result That the university's
student low level of satisfy of life and there are no different statically if
they are scientific or human in their study. And there is positive and there
is positive and indicate relation

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Publication Date
Tue Mar 05 2024
Journal Name
5th International Conference On Biomedical And Health Sciences
Antimicrobial Activity Zinc Oxide ZnO Nanoparticles Against Biofilm Formation of Uropathogenic E. coli
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Publication Date
Sun Mar 01 2020
Journal Name
Baghdad Science Journal
The Biofilm Inhibitory Potential of Compound Produced from Chlamydomonas reinhardtii Against Pathogenic Microorganisms
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Microalgae present much usefulness for antimicrobial research because of its enormous biodiversity and rapid growth rate. From this study results it is reaveled that Chlamydomonas reinhardtii were isolated from a pond of water in the province of Diwaniyah. The culture supernatants were obtained when extracted with methanol solvent. Antimicrobial activity of extracts was tested for pathogens, and the best inhibition zone obtained was against Candida albicans (32mm), S.aureus (15mm), and to E.coli (9mm). While it showed no effect against both S.epidermidis and Klebsiella spp. Biofilm was formed by all tested isolates with differences in its strength formation. The C. reinhardtii

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