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CLINICAL UTILITY OF URINARY ANTIGEN TEST AND MOLECULAR METHOD FOR DETECTION OF LEGIONELLA PNEUMOPHILA
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Background: Legionella pneumophila (L. pneumophila) is gram-negative bacterium, which causes Legionnaires’ disease as well as Pontiac fever. Objective: To determine the frequency of Legionella pneumophila in pneumonic patients, to determine the clinical utility of diagnosing Legionella pneumonia by urinary antigen testing (LPUAT) in terms of sensitivity and specificity, to compares the results obtained from patients by urinary antigen test with q Real Time PCR (RT PCR) using serum samples and to determine the frequency of serogroup 1 and other serogroups of L. pneumophila. Methods: A total of 100 pneumonic patients (community acquired pneumonia) were enrolled in this study during a period between October 2016 to April 2017; 92 samples were collected from patients attended and admitted to Al-Imamein Al-Kadhimein Medical City and 8 samples from those in the (Center of Kidney Diseases and Transplantation) in the Medical City of Baghdad. All patients were under therapy with antibiotics. Serum and urine specimens were obtained from all patients; urine samples were processed for urinary antigen test (rapid test). Serum samples were collected and submitted to DNA extraction for detection of L. pneumophila mip gene by q RT PCR assay. Results: The percentage of L. pneumophila in two hospitals in Bagdad was 30%. Of these 26% was serogroup 1 detected by urinary antigen testing (UAT). In the other hand, 23% of samples were positive by q RT PCR based mip gene, of these 19 % were serogroup 1 and 4% were another serogroup. The sensitivity of UAT is high (P value < 0.001), which means statistically highly significance than q RT PCR. Conclusion: LPUAT is a rapid tool for early diagnosis of Legionella infection, which highlights the need of using this test in hospitals and health institutions and there is a high prevalence of L. pneumophila in Iraq that refer to the necessity of considering this microorganism point of view in future studies for detection and treatment in pneumonic patients. Keywords: L. pneumophila, mip gene, quantitative real time PCR, urinary antigen. Citation: Gauad SA, Abdulrahman TR, Muhamad AK, Jawad AA, Hassan JS. Clinical utility of urinary antigen test and molecular method for detection of Legionella pneumophila. Iraqi JMS. 2018; 16(2): 207-215. doi: 10.22578/IJMS.16.2.13

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Publication Date
Wed Apr 01 2020
Journal Name
Journal Of Construction Engineering And Management
Development of Assessment Tool for Workforce Sustainability
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Publication Date
Thu Mar 05 2020
Journal Name
International Journal Of Psychosocial Rehabilitation
The history of rhythmic gymnastics for women
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Sun Jan 01 2023
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2nd International Conference On Mathematical Techniques And Applications: Icmta2021
Review of clustering for gene expression data
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Publication Date
Wed May 01 2019
Journal Name
Iop Conference Series: Materials Science And Engineering
Applications of GIS for Bridges Maintenance Service
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Abstract<p>AThe Bridge Maintenance Management System (BMMS) is an application system that uses existing data from a Bridge Management System database for monitoring and analysis of current bridges performance, as well as for estimating the current and future maintenance and rehabilitation needs of the bridges. In a transportation context, the maintenance management is described as a cost-effective process to operate, construct, and maintain physical money. This needs analytical tools to support the allocation of resources, materials, equipment, including personnel, and supplies. Therefore, Geographic Information System (GIS) can be considered as one tool to develop the road and bridge maintenanc</p> ... Show More
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Publication Date
Sat Mar 04 2023
Journal Name
Baghdad Science Journal
Exploration of CPCD number for power graph
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Recently, complementary perfect corona domination in graphs was introduced. A dominating set S of a graph G is said to be a complementary perfect corona dominating set (CPCD – set) if each vertex in  is either a pendent vertex or a support vertex and  has a perfect matching. The minimum cardinality of a complementary perfect corona dominating set is called the complementary perfect corona domination number and is denoted by . In this paper, our parameter hasbeen discussed for power graphs of path and cycle.

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Publication Date
Sun Jan 01 2023
Journal Name
Computers, Materials &amp; Continua
Industrial Recycling Process of Batteries for EVs
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Publication Date
Thu Oct 25 2018
Journal Name
Al–bahith Al–a'alami
Image of relief organizations for displaced Iraqis
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This research aims to know the intellectual picture the displaced people formed about aid organizations and determine whether they were positive or negative, the researchers used survey tool as standard to study the society represented by displaced people living in Baghdad camps from Shiites, Sunnis, Shabak, Turkmen, Christians, and Ezidis.
The researcher reached to important results and the most important thing he found is that displaced people living in camps included in this survey hold a positive opinion about organizations working to meet their demands but they complain about the shortfall in the health care side.
The research also found that displaced people from (Shabak, Turkmen, and Ezidi) minorities see that internati

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Publication Date
Thu Nov 27 2025
Journal Name
Journal Of Bioscience And Applied Research
Survey of plants for Brenaj, Wasit, Iraq
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The current study showed that the plants were collected from 23 geographical locations in Brenaj, Wasit, Iraq. The region was characterized by a great diversity of wild plants spread densely in this region. The results were as follows: 32 families, 149 species. Asteraceae was the most widespread with 29 species from the group of dicotyledons, followed by the Fabaceae family (19) species, but there are 13 plant families, with one plant species recorded for each plant family. in Brenaj, Wasit included: Aizoaceae, Capparaceae, Convolvulaceae, Frankeniaceae, Molluginaceae, Papaveraceae, Phyllanthaceae, Primulaceae, Rutaceae, Rubiaceae, Verbenaceae, Zygophyllaceae, Urticaceae, while the plant family Poaceae was most widespread in genera and spec

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Publication Date
Fri Jan 19 2024
Journal Name
Turkish Journal Of Pharmaceutical Sciences
Taste Masking of Steroids for Oral Formulations
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Publication Date
Sat Sep 30 2017
Journal Name
Al-khwarizmi Engineering Journal
Travel Time Prediction Models and Reliability Indices for Palestine Urban Road in Baghdad City
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Abstract

     Travel Time estimation and reliability measurement is an important issues for improving operation efficiency and safety of traffic roads networks. The aim of this research is the estimation of total travel time and distribution analysis for three selected links in Palestine Arterial Street in Baghdad city. Buffer time index results in worse reliability conditions. Link (2) from Bab Al Mutham intersection to Al-Sakara intersection produced a buffer index of about 36%  and 26 % for Link (1) Al-Mawall intersection to Bab Al- Mutham intersection and finally for link (3) which presented a 24% buffer index. These illustrated that the reliability get worst for link

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