This research work was conducted to study the role of cryptosporidial infection in calves with chronic non-responsive diarrhoea (1-4 weeks old). The
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The multipotent characteristic of rabbit adipose-derived stem cells makes them available and convenient sources for isolating mesenchymal stem cells. The aim of this study was to assess the differentiation in rabbit adipose-derived stem cells pre-committed to produce several mesenchymal lineages in response to inductive extracellular cues to multipotent stromal cells. Three grams of adipose tissue was taken from a subcutaneous region of the nape of the neck and was carefully isolated to obtain mesenchymal stem cells for expanded by fourth passage. In the 4th passage, active growth of mesenchymal stem cells was observed. Furthermore, the research demonstrated the inherent ability of rabbit MS |
This study is considered the first study in our country to isolate bovine parainfluenza
type 3 virus (BPIV-3) from naturally infected calves. BPIV-3 virus was isolated from nasal
swabs collected from calves with respiratory disease. The virus was isolated and titrated in
secondary embryonic bovine kidney (SEBK) cell culture; other cell cultures (secondary sheep
testis and chicken embryos fibroblast cell cultures) were used for propagation of isolated
virus. Examination of infected cell culture revealed giant cell formation and intracytoplasmic
inclusions. The isolated virus agglutinated guinea pig’s erythrocytes and produces clear
heamadsorption of guinea pig’s erythrocyte in infected SEBK cell culture. Virus tit
The present study was carried out to investigate the effects of Ochratoxin A (OTA) on the serum activity of AST, ALT and ALP, Glutathione (GSH) and liver, kidney and spleen histopathology .Twenty male rats divided equally into three treated groups dosed orally and daily with OTA doses (70, 140 and 210 μg/kg) for 20 day representing T1, T2 and T3 groups and the fourth group dosed daily with distilled water considered as control one. Biochemical result showed elevation in AST and ALT activity and decrease in ALP enzyme activity and GSH serum concentration of treated serum groups at 10 and 20 days of exposure to ochratoxin A these changes positively were proportional with increase OTA doses (dose dependent). Histopathological changes were
... Show MoreThe objective of this study was to determine the clinical effects of total intravenous anesthesia (TIVA) by romifidine 0.1mg/kg as a premedication and anesthesia by intravenous injection of a mixture of midazolam 0.1 mg/kg and ketamine hydrochloride 2.2 mg/kg in the ten health She donkeys. The maintenance of anesthesia was performed by intravenous infusion of a mixture of the midazolam 0.065mg/kg/hrs and ketamine 6.6mg/kg /hrs prepared in 500ml normal saline. Data were collected just before intravenous administration of premedication (control data) and after the administration of anesthetics drugs at 5, 10, 15, 20, 25, 30, 45, 60 and 90 minutes. The clinical parameters measured included: Anesthetic Parameter (induction, anesthetic time a
... Show MoreThe study was conducted on 35 pregnant Iraqi cows at gestation period ranged from 260-265 days depending on their date of services. This study was performed in two regions from Baghdad province (Al-Thahab Al-Abiad and Al-Nahrawan villages) from 2009-2011, there ages ranged from 3-6 years and were divided randomly into three groups according to the type of treatment. 1st group (11 cows) were injected with 750 μg (3 ml) of estrumate intramuscular (IM) in single dose .The 2nd group (13 cows) injected with 40 mg of dexamethasone (IM) in one dose only. The 3rd group (11 cows) injected with 500μg of ergometrine (IM). The results revealed that the responsive cows were 7 (63.6%), 3 (100%) and 8 (72.7%) in the 1st, 2nd and 3rd groups respective
... Show MoreFor studying in vivo influence of immune responses on side effects of Mitomycin C (MMC),Sixty-five male-white mice were divided to nine groups ;1st group inoculated MMC,I.P,0.4mg/ml,2 doses weekly/4 weeks;2nd to 7th groups immunized S/C with LM Ags(25mg/ml) protein concentration 0.4 ml, 2doses/2 weeks intervals;2nd and 3rd groups immunized CFLMAgs and CFLMAgs-MMC in 3rd group; also 4th and fifth groups but immunized with WSLMAgs; 6th and 7th groups inoculated with 0.4 ml Attenuated LM (LaLMAgs), 7th group immunized-MMC treated. Post-immunization, 5 animals sacrificed from 1-7groups and negative control to study the cellular and humoral immunity as well as bone marrow taken to cytogenic examination ,other animals from 1-7 groups and 8th g
... Show MoreUsing the rose Bengal test 114 blood samples collected from dogs , and tested for Brucella antibodies , (30.7 % ) were found positive for Brucella antibodies while (64.3 % ) were found negative for Brucella antibodies .
Key words : Brucella antibodies , dogs .
In recent years, fluorosis caused many problems in humans and animals bodies. Dental fluorosis, skeletal fluorosis, bone fractures, decreased birth rates, adverse effects on the male genital system, like damaging the structure of testes and epididymis and loosing fertilization ability can be considered as good examples of fluorosis. Thus, the current study aimed to determine the toxicity of sodium fluoride (NaF) on the activity of sperm and the level of sex hormones including testosterone, follicle stimulating hormone, and luteinizing hormone. For achieving this purpose, thirty adult albino male rats, aged between 90-100 days, were divided randomly into two treated groups with 10 rats for each group which were treated with 150, 300 ppm o
... Show MoreThe current study was carried out to investigate the immunological and hematological changes due to local transplantation of human umbilical cord-mesenchymal-stem cells (HUC-MSCs) and scaffold-stem cells (SSCs) into the injured radial nerve. Therefore, three equal groups of dogs were subjected to this study; experimental (EG), positive control (PCG) and negative control (NCG). At 1st week, dogs of EG were showed an obvious mobility dysfunction. At 2nd and 4th weeks, there were apparent improvements reported on general and physical activities as well as functional ability of forelimb with the presence of slight lameness that was cured completely at 5th week. Regarding to immunobiomarkers, insignificant differences were showed at 1st week.
... Show MoreEimeria is an apicomplex protozoon which affects many species of birds, domestic and wild animals including deer. This is the first study in Iraq that was designed for diagnosis Eimeria spp. in deer using traditional methods. Eighty fecal samples of Fallow deer (Damadama) were collected in different provinces in the middle part of Iraq during December 2018 to the end of September 2019. Samples were examined by direct smear and flotation with Sheather's solution. The total infection rate with Eimeria spp. was 70% (56/80), in which higher infection rate was observed in female deer (78%, 39/50). The results also showed that Fawn at age<3-11 months recorded higher rate of infection (86.3%, 19/22). Eimeria infe
... Show MoreThe purpose of this study was to describe some anatomical and radiological features of the non-lactating udder of adult local Iraqi cow. Eight udder of local breed cow clinically appeared healthy and aged between 2-5 years were obtained from a local slaughterhouse located at Baghdad province. Five udders were used for anatomical study and the rest 3 udders were used for the radiological study. The study primed to describe the macroscopic anatomy of the udder and investigate the udder’s normal blood supply using red-colored latex. The mammography method was to describe the duct system of the glandular tissue of the mammary gland which was performed using the iodine compound and barium sulphate as contrast me
... Show MoreCoronavirus (COVID-19) is suspected to originate from an animal host (zoonotic) followed by a human to human transmission, The purpose of the present study is to determine the genetic affinity of a phylogenetic tree and conformation of protein between human and bat. Through study of genetic sequencing, as shown in the tree design of strains and genetic variants, the main cause of COVID-19 is the Bat Coronavirus RaTG13 (SARS) virus in ID: MN996532.1 and ID: MG772933.1 (24-Jul-2013 and Feb-2017) shows that the evolution of the Corona virus from 2003, 2013 to 2020 which has become the most deadly peak in humans, the virus evolved from the bat effect on Humans. Protein analysis show 98 change of amino acid form RaTG13 (SARS) virus to COVID-1
... Show MoreThe current study was established to find out the role of immunization of Pseudomonas aeruginosa-whole sonicated antigen in adult white fur domestic rabbits. To achieve this goal, fifteen rabbits were allocated into 3 groups, the first group was immunized with P. aeruginosa–whole sonicated antigen and challenged with viable pathogenic P. aeruginosa; the second group (control negative) was treated with phosphate buffer saline and the third group was injected with viable pathogenic P. aeruginosa (control positive). The results demonstrated increasing levels of the measured parameters blood picture (total WBCs, lymphocytes, and granulocytes, RBCs and hemoglobin concentrations) in the first group compared with
... Show MoreThe aim of this study was to reveal the blood supply of the intestinal tract in male adult turkey. Five healthy birds were collected from local suppliers at Baaqoba province. All birds were euthanized and their coelomic cavity was dissected. The descending aorta was cannulated and injected with colored latex, and then the course of arteries along the intestinal tract investigated. Small intestine received the blood by celiac artery, cranial and caudal mesenteric artery. Celiac was split into two branches right and left, the right branch of celiac artery supplied the proventriculus, gizzard, duodenum, pancreas, jejunum and distal part of ileum and cecum while left branch supply the stomach. The crania mesenter
... Show MoreWater intoxication is a fatal disorder associated with disturbance in brain function, known as hypo-osmolar syndrome which resulting from an excessive water intake, with dilutional hypernatremia leads to a potentially fatal outcome. A change in the electrolyte equilibrium such as this sudden drop in serum sodium level and then subsequent mortality. With hyponatremia, the plasma osmolality decreased leading to water movement into the brain according to the osmotic gradient, resulting in hyponatremic encephalopathy and cerebral oedema. Increased water intake such as in Psychogenic polydipsia is followed by urination of high amount of diluted urine (polyuria) which are the main initial symptoms of water intoxication with headache, blurred v
... Show MoreBrown truffle (Terfezia claveryi) is a wild fungi species collected and consumed by humans in Iraq, especially during the raining season, from February to April. However, the toxicological effects of this fungus have not been studied in humans. This study tested the subacute toxicity of brown truffle’s methanolic extract on a rat model. Daily oral doses of 200, 400, and 800 mg/kg were administered to adult Sprague-Dawley rat groups of both sexes for 14 days. There were no behavioral changes, no alterations in body weight, organ weight, and body weight gain (p>0.05) in the treated rats, compared to the untreated control group. The hematological and serum biochemical parameters did not show significant (p>0.05) differences from the
... Show MoreTrypanosomiasis is one of the common parasitic diseases, which infects the dromedary camels and decreases the numbers of these animals in Iraq. To get the best knowledge of the changes of iron status in camels infected with trypanosomiasis, in an attempt to take advantage of these variables as markers for infection, we designed this study. The current study conducted in blood sample collection from155 dromedary camels, 33 were infected (21.29%), according to the status of infection with Trypanosoma evansi that depends on blood smear examination as a golden test. Results denote significant differences of infection ratio by sex and age, from total of 132 male tested, 29 (21.96%) infected, distributed into 12 (%
... Show MoreLiver biopsies were taken from (89) cattle slaughtered at modern Baghdad abattoir. Macroscopic examination of livers showed that (28.11%) of cases were infected with chronic fascioliasis, while (1.12%) of livers showed acute fascioliasis and (2.24%) of livers showed chronic fascioliasis with hydatidosis. Hydatid cysts were present in (6.74%) of inspected livers, telangiectasis of the livers found in (3.37%) of cases, focal hepatic necrosis (Saw dust) was observed in (19.1%), liver abscesses constituted (1.12%) and (38.2%) of livers had no significant gross lesions. The diagnosis of liver diseases in cattle using liver biopsy technique was more successful when the lesion was extensively diffuse in the liver.
Through a full calander year a total of 2220 (1089 male and 1131 female)
... Show MoreA study on the prevalence of gastrointestinal parasites of horses in Baghdad area extending over a period of one year , revealed two small peaks in worm egg counts , one in spring and the other in autumn and treatment routines should be based on this information. During the hot and dry summer months of Iraq, helminth infection was at low level.
Faecal culture for larvae and their identification indicated the predominance of small red worm infection in the horses.
A six months survey (2-8/1994) was canied out to determine norcaiidal mastitis in cattle in certain dairy herds. A total 126 milk samples collected from mastitic‘ cows and milk tank. Of which 10 from Al-Khalis station, 15 from A1-Wahda station, 6 from White Good village, 90 from Al-Dejaila station and 5 fromMilktauk. Examination of the samples result in isolation of Norcaitlia asteroides from Al-Dejaila station.
The results explained that 25 animals were positive for norcaridal infection with prevalence of 20.8 % of these 25 cases, 5 cases, in pure cultural and 20 cases, mixed with other organisms. Bacteriological examination of mill: tank also resulted in positive for norcaridial isolates.
Metastasis to the lung and su
... Show MoreThe complement fixation test (CFT) and the direct fluorescent ant
... Show MoreThe thyroid gland of Moorhen has two separated lobes. These lobes were located
... Show MorePancreatic islets of Turkey were different from those of mammals. It is divided into alpha, beta and mixed types according to the ground of cellular composition
This study was carried out at the Iraqi ministry of science & Technology as well as the Vet. Collage- Baghdad. This research aimed to study the effects of macroscopic and histological licorice extract in the genital tract of ewes as well as to study the effect of the extract on reproductive efficiency to be compared with serum hormone PMSG( pregnant horse) used for this purpose. The study used (36) local ewes which are divided into three equal groups (T 1, T 2, T 3) (Licorice extract 300, PMSG 500 and zero respectively), The first group( T1) of ewes was given licorice extract a single dose of 300 mg / kg body weight weekly for a period of ( 6) weeks, The second group (T 2), they were treated with hormone PMSG with a dose of 500 unit
... Show MoreThe study was designed for isolation of Staphylococcus aureus from raw milk of cows at different regions of salah al-din province. A total number of 109 samples were collected form October (2010) to May (2011). The result of study showed that the number of isolates were (14) from (60) positive samples (82.22%). Antibiotic susceptibility test showed different results for susceptibility according to types of antibiotics that used in this study which include the followings: Amoxycillin, Ampicillin, Cefoxetine, Methcillin, Ciprofloxacin, Clavulinic acid and Erythromycin. The results showed that 50% of the isolates were susceptible for Methecillin, Cefoxetine, and Clavulanic acid-Amoxycillin), While Erythromycin was active against (79%) of th
... Show MoreThis study was conducted to evaluate the effect of adding different levels of rocket salad seeds powder to the diet on seminal plasma traits of roosters subjected to oxidative stress induced by hydrogen peroxide. A total of 60 Hy – line laying breeder roosters 57 weeks old were used in this study. Roosters were randomly distributed into 5 treatments with 3 replicates each. Each replicate constituted of 4 roosters (12 roosters for each treatment). Experimental treatments were as following: T1: Males fed control diet and normal water, T2: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet + 0.25 ml hydrogen peroxide (0.5%) / litter of water, T3: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet +
... Show MoreFourteen Bulgarian pregnant ewes (three months of gestation), were used and divided in two groups, one group was inoculated with 500 infective larvae /kg. body weight, the other group remained non-infected control. Productive parameters, blood changes were recorded and faecal egg counts were examined weekly. Marked reduction in food intake was recorded in infected ewes, especially during prelambing period. No obvious differences were noted between both groups after lambing. Slight differences in body weight gain were recorded between both groups until labour, while after lambing, the weight losses of infected ewes were highly significant 1%. No effect of infection has been seen on fleece weight, while significant depression in the haemog
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Footrot is a significant health problem in ruminants, especially sheep, caused by the bacteria Dichelobacter nodosus and Fusobacterium necrophorum. Infected animals may become lame and lose body condition. The aim of this study was to investigate whether sheep had mixed infections with D. nodosus and F. necrophorum, the global genetic relationships of both target genes (lktA and 16S rRNA genes) with different reference genes, and the degree of genetic heterogeneity between the standard serogroups of D. nodosus were studied. In addition, to detect the antibacterial sensitivity against these two pathogens. One hundred and sevent |