The present study aimed to synthesize selenium nanoparticles (SeNPs) using aqueous extract of black currant as a reducing agent. The green synthesized black currant selenium nanoparticles (BCSeNPs) were identified by color change. The characterization of SeNPs was achieved by Ultraviolet-visible (UV–VIS) spectroscopy, scanning electron microscopy (SEM), X–ray diffraction analysis (XRD), and Fourier transform infrared spectroscopy (FTIR). These tests were used to detect: stability, morphology, size, crystalline nature, and functional groups present on the surface of BCSeNPs. The results revealed appearance of the brick-red color indicating the specific color of selenium nanoparticles, and UV-Vis spectroscopy showed band absorbance at
... Show MoreSixty samples of bursa of Fabricius were collected from broiler chickens suspected to be infected with infectious bursal disease virus (IBDV) in different areas of Iraq for molecular evaluation. The extracted nucleic acid was amplified using reverse transcriptase polymerase chain reaction (RT-PCR) targeting genes of segment A (Vp2, Vp3, Vp4 and Vp5 genes) and segment B (VP1 genes). The products of amplification were sent to Korea for sequencing using Sanger method. The sequencing analysis of the IBDV from the Iraqi isolates revealed that each gene had different transition and transversion (nonsense and missense of point mutation) compared to reference genes. The phylogenetic tree analysis showed that the VP2
... Show MoreAn indirect ELISA technique was used to determine the prevalence of infection in sera and milk samples among goats and sheepin Duhok, Kurdistan Region, Iraq. Results of the present study shows that the prevalence of infection was higher in sera compared to milk samples among goats and sheep as follow: (6.3, 4.2%) and (8.9, 5.6%) respectively and there was no statistical significance difference in goats at P value =0.42, also there was no statistically differences at P value= 0.22. ELISA results also shows that the prevalence according to the age among goats and sheep were higher in animal aged > 2 years in both sera and milk samples were (7.5, 5.3%) and (11.3, 7%) respectively and there was no significant difference between age groups
... Show MoreTrials had been made for synchronization of estrus in both ewes and goats during Summer and Autumn seasons
... Show MoreThe parotid salivary gland in one humped camel (camelus dromedarius) is located along the caudal edge of the ramus of mandible and on the masseter muscle. The gland has a long triangular outline and a red-brown color. The lateral surface is covered by parotid fascia and the cutaneous and parotido-auricularis muscles. It is bounded dorsally by zygomatico-auricularis muscle. The gland takes its blood supply from three branches which come from the facial artery that supply the middle and distal part of the gland, while the proximal part receives its blood supply from the superficial temporal and cranial temporal arteries. The parotid duct of the gland passes over the lateral surface of the masseter muscle, infer
... Show MoreThe aim of the present study was to investigate the protective role of Salvia officinal's silver nanoparticles as antioxidant on nephrotic damage induced by methotrexate in adult rats. Green silver nanoparticles were synthesized using alcoholic extract of salvia officinal's leaves, and were characterized by UV-spectrophotometry and scanning electron microscope. The mixing of the plant extract of Salvia. officinal's with silver nitrate solution (1mM), lead to changing of the reaction mixture color to yellowish within one hour and to dark brown after 8 hours, indicating the generation of Salvia officinal's silver nanoparticles , due to the reduction of silver metal ions silver (Ag+) into Nano silver particles via the active compounds
... Show MoreA study was dane on 300 cases of Colibacillosis in broiler chickens collected from poultry farms in Baghdad. Escherichia coli was isolated from all cases from heart blood. The highest incidence (45%) of the disease was noticed among the 3-4 weeks old chickens. The antibiotic sensitivity test revealed that all isolates were resistant to Chloramphenical, Tetracycline,Lincomycin and Erythromycin. Ninty five percent of the isolates were resistant to Sulfa Trimethoprim mixture and 40% to Ampicillin. Serotyping of some of the isolates showed that the serotypes 01, 02 and 078 were the most prevalent types; other serotypes were also identified especially 035, 036, 088 and 018.
Chick embryo dermis (CED) cell cultures were prepared from skinpeeled off from 12 day old specific pathogen free (SPF) embryo. The
... Show MoreA study was conducted invitro to determine the effects of storage at
temperature 4 Cº upon the survival of protoscolices of Echinococcus
granulosus from 86 hydatid cysts isolated from infected donkeys . Flam cell
activity was the choice for viability , in addition using vital stain (0.1%
aqueous eosin) . The result showed that higher percentage survival 94.41%
in hydatid fluid for 9 days . The longest survival times of any of the samples
tested was 81 days with the survival percentage 85% and the lowest was 33%
for 13 days , showing movement till the end of this period . Our results
proved the protoscolices removed from cyst retain their survival for several
weeks at refrigerator temperature when stored in hyd
Experimental study albion mice as a host was conducted in on attempt to
... Show MoreThe indigenous geese have symmetrical pair of ultimobranchial gland, pink in color, lenticular or globular in shape. These glands are located in thoracic inlet, posterior to the parathyroid gland, dorsal to the bifurcation of brachiocephalic trunk. In young birds the left gland usually adheres to the caudal pole of parathyroid gland and usually cranio lateral to the origin of common carotid artery, while the right gland is also related to common carotid artery, but it’s separated from caudal pole of parathyroid gland. The gland is supplied by collateral branch from common carotid artery and innervated by branch from the thoracic vegal trunk and directed from the recurrent nerve. The gland is not surrounded by capsule but it’s covered
... Show MoreEimeria is an apicomplex protozoon which affects many species of birds, domestic and wild animals including deer. This is the first study in Iraq that was designed for diagnosis Eimeria spp. in deer using traditional methods. Eighty fecal samples of Fallow deer (Damadama) were collected in different provinces in the middle part of Iraq during December 2018 to the end of September 2019. Samples were examined by direct smear and flotation with Sheather's solution. The total infection rate with Eimeria spp. was 70% (56/80), in which higher infection rate was observed in female deer (78%, 39/50). The results also showed that Fawn at age<3-11 months recorded higher rate of infection (86.3%, 19/22). Eimeria infe
... Show MorePowder of pomegranate barks (Punica granatum L) was prepared. Different concentration of watery
... Show MoreOne hundred and twenty Hisex Brown commercial layers at the age of 40 weeks were divided into 3 groups each was subdivided into 4 replicants. Each group was given one of three diets contained either 0.54, 1.04 or 1.54% available phosphorus and 3.0% calcium for one month. Egg production was recorded, and eggs and shells were weighed. Group 2 produced more egg (12.18 egg/day) than group 1 (9.31 egg/day) and group 3 (9.3 egg/day). Groups received higher percentage of available phosphorus in their diets produced heavier eggs (65.14, 67.67 and 68.28 g/egg respectively). Egg shell weight was increased accordingly when available phosphorus in the diet was raised (7.88, 8.85 and 9.0 g/egg respectively). It was concluded that the available phosph
... Show MoreThe thyroid gland of Camelus dromedrius is located directly behind the larynx. It consists of two lateral lobes at the sides of trachea. The lobes are connected to each other by a caudal isthmus from the ventral surface. The lobes extend to the seventh tracheal ring and even to the eighth or ninth ring. The medial surface of the lobes is adhered to trachea. The lateral surfaces are convex. Also, the dorsal and ventral edges of the lobes are convex.
The thyroid gland receives blood via caudal thyroid artery and cranial thyroid artery. A third artery which is initiated from the common carotid artery serves as a medial thyroid artery.
Histological observation showed that the lobes and isthmus are surrounded by an external ca
... Show MoreThe present study was designed to investigate the antinociceptive activity of seperate and
combined treatments of ethanol extract of Zingiber officinale rhizomes and vitamin C on
visceral nociception induced by intraperitoneal injection of 2% acetic acid which produced
writhing in rats. The preliminary chemical tests were performed on the extract and revealed the
presence of flavanoids, alkaloids and tannins. Visceral nociceptive responses including the
latency time to beginning of the first writhe, number of writhes per hour and the inhibition
percentage of writhing. Seperate i. p. injection of ethanol extract of Zingiber officinale at dose of
25 and 50 mg/kg BW caused significant (P<0.01) increase in latency tim
Spores of Bacillus subtilis HU1 suspended in whole milk and /or distilled water subjected to high and ultrahigh temperatures ranging from 85 to 14°C. Resulting survivor and thermal death time (TDT) curves were shown to be non-linear for all temperatures investigated; convex survivor curves at most heating temperatures were observed. TDT curves observed in all studies were concave and biphasic. The z-value using milk as the menstruum were 57°C for heating temperatures of 121-140°C, and 20°C, for heating temperatures of 110-121°C. Distilled water as the menstruum yielded z-values of 58°C and 16°C for heating temperatures of 120-140°C and 110-121°C respectively.
This study was designed to explain the anatomy and histology of kidneys and salt glands in Great Flamingos. Eight adult healthy Great Flamingos of both sexes have been used. The results showed that the kidneys in Great Flamingos consist of three separated lobes. The right kidney was longer 8.9±0.3 mm than the left kidney 8.4±0.4mm. The ratio of the kidneys weight to the total body weight was 0.39%. The salt gland was very large crescent shape, and occupied the supraorbital fossa and has 20.8±0.2mm long and 3.9±0.2mm in diameter. The volume of cortex was 60-70% and the medulla was 30-40%. Within the middle region of cortex there were numerous large corpuscles (mammalian type) and few of small corpuscles (repti
... Show MoreAutopsy study of 218 (106 male and 112 female) animals slaughtered in Baghdad ( Al-ZAWRA ) Zoo, of central Iraq (Diyala, Wasit, Baghdad) .The total infection rate of Setaria equina was 11.9% and the highest infection rate (22.2%) was recorded in October, where is no infection in January, February and August. It has been observed that the high infection rate (14.5%) during the months of spring and autumn (18.8%) and decrease in winter and summer 3.6% and 10%. Female donkeys with a higher proportion (13.5%) than male10.3% respectively differences ( P < 0.05 ).Worm intensity ranged between 1-6 worm and was shown high worm burden in Autumn and Spring fallowed by Summer and finally Winter seasons, which no infection was recorded in some mo
... Show MoreSynergistic action of probiotics and prebiotics (synbiotic) has been suggested to be more effective than the two separate components in the prevention and treatment of many intestinal and immune diseases. The present study aimed to examine the anti-inflammatory role of Fenugreek as synbiotic with Lactobacillus acidophilus against Escherichia coli. Twenty four adult males of Wister rats aged 3-4 months and weighted 200-250 gm were used and divided into 4 groups: 1st and 2nd groups were negative and positive control (C and C++) fed with basal diet, the 3rd group (T1) fed diet with Fenugreek seeds (5%) and the 4th group (T2) fed with the synbiotic Fenugreek seeds (5%) and L. acidophilus (5 × 108 CFU/ml) for 45 days. After that, rats in the
... Show MoreThis research aims to prepare ZnO NPs by using chemical bath deposition way from ZnSO4 and NaOH as starting materials. It was examined by X-ray diffraction, Scanning Electron Microscopy, Zeta potential and Fourier Transformation Infrared. Scanning Electron Microscopy images showed various morphological changes of ZnO nanoparticles obtained by the above method and the different magnification Scanning Electron Microscopy images of the nanoparticle and confirms that the Nano flowers are grown with well-defined morphology and diameters varying between 60-110 nm. The effect of Zinc oxide nanoparticles against bacteria staphylococcus aureus, E.coli and Pseudomous aeruginosa showed
... Show MoreThis study represent a new process for dehydration of the histological
specimen that knowledge and used in the laboratory study . Technical and
medical research will be set up a completely great scientific and to addition of
this to make an experiment , to a shortness the time and the material cost that is
used in the different stage of dehydration and these for the soft tissue ( only ) .
In order to study the pathological lesion in some internal organs of wild
rats as well as bacteriological and parasitic isolation. Post-mortom examination
of 45 wild rats collected from different parts of baghdad city was done. The rsult
showed that 29 rats from 45 were infected with Cryptosporidia spp , and
Hymenolips spp . Bacteriological and pathological examination revealed the
following bacterial Isolate and pathological changes :
1. Sub acute suppurative hepatitis , Klebisella spp, Salmonella typhimurium
were isolated from this lesion .
2. Granulomatus hepatitis with isolates of Ps.aeroginosa , E. coli and Kl.spp.
3. Acute Fibrinous pericarditis with isolates of Ps. Aeruginosa, Strep.
Pyogens.
4. Ne
This study was carried out in the laboratories of fish for 90 days in 16 glass aquaria (60 cm, 40 cm and 30 cm/70 L) to investigate the effect of some organic acids salts on some growth indices of common carp fish Cyprinus carpio L. A 128 fish were used with an average weight of (40.8±1) gm. and have been distributed on eight treatments with two replicate and eight fish for each replicate. Formulated eight diets that equally iso-nitrogenous (33.85%) and iso-caloric (1535.605 KJ/gm) were investigated. Organic acids salts (Sodium lactate, Sodium acetate and Sodiumformate) were supplemented singly (0.5% of each salts) and mixed to the experimental diets (0.25% of each salts). The statistical results showed
... Show MoreThe present study were made for analysis of major chemical and biochemical components of twenty hydatid cysts fluids include two group (10 fertile cysts and 10 sterile cysts) obtained from the lung and liver of 29 infected patients admitted to medicine city hospital for operation during a period of 6 month these components were measured by colorimetric assay kits (Biolabo®, Human). Results revealed marked (P<0.05).differences in the mean values, particularly among enzymes (Glutanicoxaloacetic transaminase, Glutanic pyruvic transaminase and Alkaline phosphatase), between fertile and sterile fluids while Lactate dehydrogenase level was exhibited high activities in fertile and sterile iso
... Show MoreThis study aimed to evaluate the efficacy of different hormonal treatments protocols (PGF2α ,GnRH, estradiol and progesterone) hormones on reproductive performance of postpartum anoestrus native dairy River buffaloes (Bubalus bubalis), endemic south of Baghdad under field conditions . Present study was conducted on 128 animals that had postpartum anoestrus (PPA) for a period between 4 to 8 months. The animals were subjected to two experiments according to the type of anoestrus. In the first experiment 94 animals (73.5%) with persistent corpus luteum on their ovaries without any signs of estrous (sub-oestrus) were classified into two sub-groups.Sub-group1 (n=47) treated with PGF2α hormone alone and sub-group 2 (n= 47) were treated by tw
... Show MoreThe aim of the present study was to compare the effect of two probiotic
products, Biomin and Iraqi Probiotic, on some blood and biochemical properties
and intestinal microflora population in broiler chicks. A total of 500 Lohman
broiler chicks, one day old, were randomly distributed into five dietary groups as
follow:
T1: chicks fed on diet with half dose of Biomin (1.5 Kg/ Ton diet).
T2: chicks fed on diet with full dose of Biomin. (3 Kg/ Ton diet)
T3: chicks fed on diet with half dose of Iraqi probiotic. (2.5 Kg/ Ton diet)
T4: chicks fed on diet with full dose of Iraqi probiotic. (5 Kg/ Ton diet)
T5: chicks fed slandered diet. (Control)
Results obtained from the present study showed that both probiotics we
Hydatid Cysts were obtained from 15 cows from liver, lung, spleen, heart, and peritoneal cavity, between December 2014 and October 2015. Hydatid cysts (protoscoleces) were used for deoxyribonucleic acid extraction by using mechanical grinder. The purification of mtDNA was done by (promega kit, USA). The mitochondrial NADH dehydrogenase subunit 1 (ND1) genes was used as targets for polymerase chain reaction amplification, all hydatid cysts yielded amplification products. Polymerase chain reaction product for NADH1 800 basic pair. The polymerase chain reaction products were purified and partial sequences were generated. The sequences obtained were found to align with corresponding region for ND1 g
... Show MoreThe aim of this study was to evaluate virulence of local isolated avian infectious laryngotracheitis virus in experimentally infected chicken. Forty chickens 10 weeks old were used for the experimental infection with the locally isolated infectious laryngotracheitis virus. Chickens were divided into three groups, the first group consisted from 20 chickens infected with isolated infectious laryngotracheitis virus (2×104.16 TCID 50/50 µl) via eyes and mouth drops (one drop for each). The second group consisted of 10 chickens (non-infected) in contact with infected group inoculated with maintenance media (Minimum essential medium) on their eyes, to observe if the infected group can spread the virus. The
... Show MoreSingle cell protein was used to replace 0,7,10,13 and 16% of soyabean meal in diets for
... Show More