The present study was conducted during the period from September 2015 until February 2016. 100 fecal samples were collected from 60 sheep and 40 goats for diagnosis of Cryptosporidium parasite from diverse areas in Al-Qadisiyah province. The study amid to know the genetic characters of Cryptosporidium spp. parasite by using a molecular technique such as the nested polymerase chain reaction and DNA sequencing analyzed by phylogenetic tree to identify the parasite species. This study was done on the sheep and goat at first time in the middle region of Iraq and the identified species were recorded in NCBI-Genbank database. In sheep, the results of positive infected samples was (40%) while, in the g
... Show MoreThe aim of this study was to investigate the effect of anti-riboflavin factor in dried tomato seed meal on some physiological and biochemical traits of broiler. This study was conducted at the poultry farm of the Veterinary Medicine College at Baghdad University. A total of (150 broiler chicks) were used in this study for 56 days. The chicks were randomly distributed into five treated groups, 30 chicks for each period. Chicks at the age of 3 days were fed Ad libitum during the whole period and dried tomato seed meal was added to the diets of birds at the concentration of 0, 2.5, 5, 7.5 and 10% respectively. The results revealed that dietary supplementation with different levels of dried tomato seed meal
... Show MoreTestes from 700 male goats aged l - 3 years , slaughtered at Baghdad abatteir, were examined during 6 months period. Histological and bacteriological examinations were done when gross lesions were observed. The results revealed that epidedimytis was observed in 21 (3%) of the examined animals ; 16 (2.3%) cases were unilaterally affected and S (0.7%) cases were bilaterally affected.
Bacteriological isolations from infected organs included :
Corynebacterium ovis (6 isolates) and Actinomyces pyogenes (3 isolates) both constituted the majonty of isolates ; as well as E. coli (4 iso-lates), and Pseudomonas aeruginosa , Staphylococcus epidermidis, Cory. bovis., Staph. aureus, Cory , ulcerams &. Yersinia pseuclotuberculosis
... Show MoreA total of 54 broiler chicks were used to study the steady state levels of
Tilmicocin and Enroflxacin in lung tissues and plasma.
Microbiological assay, using Bacillus subtilis (ATCC 9225) or Saricinea leutus
(ATCC 272) as test organisms for Enrofloxacin and Tilmicocin respectively,
was employed to estimate the drugs levels.
The birds were divided equally into two groups at the age of 21 days.
The first group received Enrofloxacin at an estimated dose of 10 mg/kg body
weight in drinking water for three consecutive days, while Tilmicocin was given
in the same way but at a dose of 15 mg/kg body weight .Samples of lung tissues
and plasma were collected from birds of each group at scheduled periods (6, 12,
18, 2
In order to know the cross –protection between antigene of T.rubrum and
C.albicans,17 mice were used and they were divided into (3) groups,1st group(7
animals) was immunized with T.rubrum antigen,two doses, and injected S/C
with virulent C. albicans,the 2nd group (5 animals) was infected with
C.albicans and considered as positive control,3rd group was served as negative
group which injected 0.5 ml of sterile normal saline s /c The results showed
that the antigen of T.rubrum stimulated a good immune response in
immunized mice against C.albicans.
The study was conducted to find out the effect of some feed additives in
reducing the infection rate of broilers with Salmonella typhimurium.
Forty broiler chicks at one week of age were divided into 4 treatments, 10
chicks each as follows:
T1: uninfected control.
T2: Salmonella typhimurium infected + Iraqi Probiotic.
T3: Salmonella typhimurium infected + Poultrygrow 250.
T4: Salmonella typhimurium infected only.
One day after feeding on feed additives, the birds were experimentally
infected with Salmonella typhimurium through the mouth.
Results show that Iraqi probiotic did not decrease the severity of infection
with Salmonella typhimurium in comparison with those of Poultrygrow 250,
shown by the perc
An epidemiological study was done on 2 groups of children infected
with watery diarrhea in some hospitals of Baghdad (group 1: immunocompetent
outpatients, group 2: immunosuppressed inpatients).
Modified cold Ziehl Neelsen stain was used in diagnosis. The whole
oocysts antigen prepared after conduction of experimental infection in 2 calves
by using isolated oocysts from children stool, and this prepared antigen was
used in Indirect Fluorescent Antibody Technique (IFAT).
The percentage of infection in the 2 groups of outpatients and inpatients
were 21.2% and 32% respectively, and there was a difference in percentage of
infection between the 3 groups of children, the highest was 36.4% in group with
leukemia an
The evaluation of gingerol as medical entities in Iraq and Jamaica cultivated
ginger was done in this experiment. Two different types of cultivated ginger
(Iraq and Jamaica) of the same species (Zingiber officinal) were extracted to
achieve the subject of this study. Extraction was performed by two different
stages: the first stage preparative and extraction. Harvested ginger was prepared
for extraction by wash, denotation, drying, grinding, and extracting by acetone
under heating. The second stage is separation by two steps via TLC and HPLC.
The quality and quantity of oleoresin and gingerol in each step of separation
were checked by spots diameter and RF value of spot migration in TLC ginger
compounds and qua
This study was conducted to investigate the hypolycemic effect of the aqueous
extract and some active ingredients of Nigella Sativa seeds in normal rabbits. Twenty
four male rabbits were divided into six equal groups. The first five groups were received
by gavage needle 160,240,320,400 and 480 mg/kg B.W. of the aqueous extract, while
the sixth group which served as control received normal saline solution. After two hours
of administration, the group which received 320 mg showed the lowest blood sugar level
as compared to control and other treated groups. This dose was considered for the next
studies.
Lectin was isolated from Nigella Sativa seeds by 0.2M phosphate buffer containing
0.15M NaCl at pH 7.0, and the
Bacteriological , cytological, and biochemical testing were performed on synovial fluid of 160 sheep’s and goats at Basrah slaughter house , 115 sheep (74 females, 41 males ) , 45 goats (34 females , 11 males). The incidence of bacterial isolation from infected sheeps was 40.05 %. in females , 48.78 % . in males ,while in goats it was 54.54%. in males 14.7%. in females. Bacterial isolates were staphalococcus aureus, pseudomonas spp, and Hemophilus spp. Staph –aureus was senstive to impeneam and amikacin Hemophilus spp. was sensitive to cephalexin and Ampicillin , all Pseudomonas spp. isolates were sensitive to penicil
... Show MoreThis research aims to prepare ZnO NPs by using chemical bath deposition way from ZnSO4 and NaOH as starting materials. It was examined by X-ray diffraction, Scanning Electron Microscopy, Zeta potential and Fourier Transformation Infrared. Scanning Electron Microscopy images showed various morphological changes of ZnO nanoparticles obtained by the above method and the different magnification Scanning Electron Microscopy images of the nanoparticle and confirms that the Nano flowers are grown with well-defined morphology and diameters varying between 60-110 nm. The effect of Zinc oxide nanoparticles against bacteria staphylococcus aureus, E.coli and Pseudomous aeruginosa showed
... Show MoreThe aim of this study was concerned on the prevalence of Listeria spp.in the ice cream samples collected from the different pastry shops and downtown stores of Baghdad province during December 2009 till April 2010. A total of 50 ice cream samples (25 locally produced samples from raw and imported powdered milk and 25 imported samples from different origins) were analyzed and processed according to the standard protocols of Food and Drug Administration (FDA) and International Organization for Standardization (ISO) with some modifications. Growth of Listeria spp. was determined in 7 (14%) of total samples as 4 (16%) isolates from locally produced ice creams , from which one isolate was confirmed as Listeria monocytogenes, and 3 (12%) isola
... Show MoreThis study was carried out to study the toxic effect of smut wheat on male reproductive system of mice. The experimental study was performed in the laboratory using 20 mice, which divided into two groups, one group feed on normal diet, the other fed on a mixture of 50% normal diet with 50% smut wheat. A histopathological study was done on the testes of Mice fed on smut wheat for 30 days. The histopathological study revealed thickness of basement membrane of semiriferous tubule of mice, and desquamation of the sertoli cells and spermatogonium from the basement membrane of seminiferous tubule and has adversely effect on male fertility.
In order to demonstrated the pathologic changes and immune response of Brucella aboruls strain 19. Vaccinated guinea pigs were experimentally infected With Nocardia asteroides, 24 guinea pigs of both six were divided into four equal groups. The first group was inoculated sub-cutaneously with Brucella abortus strain 19 vaccine. The second group was inoculated inuapulmonary with 1 X 103 C.F.U of viable Nocardia asteroides. Thirty days post-inoculation the first, second and third groups were challenged intrapulmonary with 2.5 K 109 C.F.U. of Nocardia asteroides, the last group served as control. The results showed that mortality was 100 % in non~ vaccinated -animals, 33.4 % in immunized animals with Nocardia asteroides and zero in Brucella
... Show MoreThis study was conducted to detect and quantify three mycotoxins Dioxynivalenol, Nivalenol and Fumonisin B2 in seeds of seven wheat cultivars planted in 17 wheat fields in Basra province, Iraq. This was done by using High Performance Liquid chromatographs analysis. The results revealed that Fumonisin B2 was the predominant mycotoxin, which present in 10 fields. The lowest concentration rate of this mycotoxin was 110 µg/Kg and the maximum was 11,228 µg/Kg. Dioxynivalenol as a trichothecene was in the second level detected in 6 fields with a minimum concentration of 8 µg/Kg and a maximum of 1,060 µg/Kg. Nivalenol was found only in 4 fields ranging from 272-1900 µg/Kg. Fumonisin B2 Only three fields showed co-occurrence of
... Show MoreOut of 107 COW milk samples, 44(41.12%) yielded bacterial growth. The isolates were identified according to their cultural, morphological and biochemical characteristics as Staphylococcus aureus
This study was carried out in Artificial Insemination Center of Iraq to revealed FMD disease effect on some seminal attributer parameters of 14 imported Holstein bulls divided to three groups according to different reproductive efficiency (four High, five medium and five weak). Results showed that FMD disease had significant (P < 0.05) adverse effect on most seminal attributer parameters, mass, individual motility and sperm concentration / ml during post disease in first of two, four, all months of high, medium and weak semen quality bulls respectively .but semen volume didn’t influenced significantly with this disease. So semen collection should be suspended until resume normal fertility of sperm, after two, four month of high and
... Show MoreAcetaminophen also called paracetamol is commonly used as analgesic and antipyretic agent which in high doses causes liver and kidney damage in man and animals. Nigella sativa oil have antioxidant properties. Thirty adult male rats were used and randomly divided into three equal groups. Group (A) untreated and served as control group; Group (B) rats were orally intubated (by gavages needle) acetaminophen suspension (150mg/kg B.W). Group (C) rats were given orally acetaminophen suspension (150mg/kg) plus 1ml/kg B.W of Nigella sativa oil for 42 days in both treated group. Fasting blood samples were collected at 21 and 42 days of experiment to study the following parameters: Serum creatinine
... Show MoreThe present study is designed to investigate the histological structure of large intestine of two species of Iraqi birds which belong to two different orders which vary in their kinds of food. Adult birds common kestrel (Falco tinnunculus) that belong to carnivorous and white-eared bulbul (Pycnonotus leucotis) which belongs to omnivorous. This study was carried on 10 birds (5 common kestrel and 5 white-eared bulbul) used for histological study of the ceca and rectum in the two species of birds that include the determination of ceca and rectum, measurements of height columnar cells, villus height, and the thickness of wall tunics. Different types of stains were used including Haematoxylin and Eos
... Show MoreThis study is conducted to detect lumpy skin disease virus in Babylon, Al-Qadysia and Al-Muthana governorate during autumn 2014 using conventional polymerase chain reaction. A total of 150 specimens: 50 whole blood samples, 50 skin nodular biopsies and 50 tick samples were collected from infected animals of different breeds, genders and ages during lumpy skin disease outbreak. The results revealed that 104 cases (69.33%) were positive for lumpy skin disease virus by using polymerase chain reaction, with significant (P<0.05) differences between positive and negative cases. Out of 50 blood samples, 22 cases (44%) were positive for lumpy skin disease virus. The nodular skin samples collected from slaughtered anim
... Show MoreThe present study is assigned to evaluate the efficiency of hernioplasty in reconstruction of experimentally induced ventro-lateral hernia in bucks using two types of sutures (silk and polypropylene) and to find which thread is more safe and suitable for closure of the hernia, based on ultrasonographic examination.
Sixteen adult local breed bucks were enrolled for this study. All subjected to inducing artificial ventro-lateral hernia (12x10) cm in the right flank under the effect of local anesthesia (2% Lidocaine), then left for one month. After that, animals divided randomly and equally into two groups. In the first group, hernia repaired with silk, while in the second group, hernia closed by polypropylene. Sewing technique is used i
As a part of a protocol ( Z2F10 – Laidlomycin calf - fed Holstein health management ) Coccidiosis was studied in two hundred Holstein steer- calves (114 kg ) to evaluate the effect of management and treatment programs. Early weaned animals were shipped to the University of California Desert Research Center El Centro on the 26th of May 2010. The calves were vaccinated and treated with internal and external anti parasitic also injected with Vital E - AD (5ml SC 100000 vitamin A/ml and 12ml of liquamycin. Steers were balanced by weight and assigned within weight grouping to (40) pens ( 5 steers / pen ). Animals were randomly divided into four equal groups, the 1st was control group and the 2nd, 3rd and 4th groups were treated with Monensi
... Show MoreFrom March to November 2004 a total of 634 stool samples from the children coming to the
hospitals, cure centers and randomly from the children of kindergarten and primary schools. General
stool examination and precipitation methods were performed on each specimen, the infection rate of
Hymenolpes nana 1.8% among children for both Gender(male and female) for different ages that had
been recorded the highest rate for (4- 6)year is 2.94% and the lowest rate for (9-12)year is 1.4%.
Seasonal variation were discussed, that August was recorded the highest rate 5.8%.
This study was aimed to investigate the load of bacterial contaminant in fresh meat with different types of bacteria.One handered and seven samples were collected from different regions of Baghdad . These samples included 37 of fresh beef 70 of fresh sheep meat. All samples were cultured on different selective media to identitfy of contaminated bacteria .The result revealed that The percentage of bacterial isolate from raw sheep meat were,23.8 % of StreptococcusgroupD, 29.4 % of Staphylococcus aureus ,14.7 % of E.coli 4.9 , % of Salmonella spp, , 3.5 % of pseudomonas aeruginosa14.7 , % . 14.7 % of Proteus spp. 2.1 % of Listeria spp while the raw beef meat content 5.55 % of Staphylococcus aureus, 8.14 % of Streptococcus group D , 1.85 % 5
... Show MoreDuring the period from November 2007 untill October 2008, a total of 255 specimens of the cyprinid fish Cyprinus carpio Linnaeus, 1758, were collected from fish markets east of Baghdad city. Microscopic examination of these fishes revealed that they were infected with five species of ciliated protozoans [Chilodonella cyprini with 1.6% percentage of infestation, Ichthyophthirius multifiliis (3.5%), Trichodina domerguei (21.6%), Apiosoma piscicola (0.4%) and Epistylis solidus (1.2%)]. C. carpio is considered as a new host (third host) in Iraq for Epistylis solidus
The present study aimed to compare between the amniotic and pericardial membranes for the wound dressing models. Ten local breed rabbits have been used in the current study. They were divided into two main equal groups: Experiment (Amniotic and pericardial membranes) and control group. According to healing periods each group was subdivided into four subgroups (7, 14, 21 and 28) days post wounding. Specimens of amniotic membranes were collected from full term pregnant cows, and pericardium was obtained from abattoir immediately after slaughtering. The study was conducted on skin wound for experiment application site as full-thickness skin wounds (3×3 cm) were done on the dorsal thoracic sides. Histopatholog
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Asthma is a chronic and complex condition of the respiratory tract, that occurs in both children and adults. Currently available medications have side effects; therefore, new therapies with fewer side effects are needed. Thus, present study was designed to evaluate the potential anti-asthmatic effects of alcoholic extract of Ammi majus on ovalbumin-induced asthma in mice. Forty-eight female Albino mice were randomly assigned to one of six groups with 8 animals each. Group I received distilled water as a negative control, Group II received ovalbumin as a positive control, Groups III and IV received Ammi majus orally at 64 and 128 mg/kg/day, respectively, with sensitization, and Groups V and |
Electrophoretic Pattern of Serum proteins were studied in thirty normal Arabian race horses. The resolution of the serum protein fractions in 24 (80%) of horses, group I revealed five fractions, albumin
... Show MoreEighteen infertile multiparous mares were used in this study. They had been barren for at least 2 years and had been bred repeatedly during those years, and were 8-20 years of age. Clinical genital, and endometrial cy
... Show MoreSixty sheep of three local breeds (Awassi, Hamdani, Karadi) of age, 6-8 months were divided to two groups, the first fed on low level of nutrition. Each group then divided into Two subgroups,
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