The main objectives of this study is the separation of X from Y bearing epididymal spermatozoa of local buck by swim-up, and the use of this spermatozoa for in vitro fertilization to determine the percentage of produced male and female embryos. The sex of produced embryo was identified by polymerase chain reaction. Testis of the local buck were obtained from Al-Shu'alah abattoir and the epididymal spermatozoa were harvested from the cauda by and submitted to in vitro maturation prior to separation of X from Y bearing spermatozoa and prior to their use for in vitro fertilization. For the separation of epididymal spermatozoa, swim-up technique was used with centrifugation at 200×g or 300

إن الهدف من هذه الدراسة هو لمعرفة تأثير المستخلص الفينولي لبذور نبات القرنفل على نمو البكتريا الموجبة والسالبة . أجري الكشف الكيميائي الكمي والنوعي للمركبات الفعالة في المستخلص الفينولي لبذور نبات القرنفل وبأستخدام تقنية الكروماتوكرافي ، Eugenol( ) أظهرت النتائج احتوائه على المركبات التالية HPLCالسائل عالي الأداء ( Caffeic acid، Kaempferol ،Vallinic acid، Ferulic acid ، Ellagic acid) ،Chlorogenic aci .تم تقييم الفعالية التثبيطية للمستخلص Borneal، d ا

The prevalence of house mice (Mus musculus) Hymenolepiasis was determined in Baghdad, Iraq to study the effects of location, sex, and months on the infection rate of Hymenolepis spp. in house mice. Fifty house mice were captured from Abu Ghraib and Al-Ameriya, Baghdad, Iraq and examined for detecting parasites in laboratory in College of Veterinary Medicine, University of Baghdad. The total infection prevalence of intestinal parasites was 11 (22%) out of 50 samples, the higher prevalence was 28.57% and found in Abu Ghraib area, while lower was (13.63%) and recorded in Al-Ameriya area. The study revealed that the house mice were infected with two species of Hymenolepis: Hymenolepis nana 4 (8%) and Hymenolepis

The aim of present study was to evaluate the possibility of teratogenicity in rats when exposed to zinc chloride (ZnCl2) pre and post pregnancy. To achieve this goal, a total of 40 mature Albino Wistar female rats were divided equally into four groups as follows: T1, dosed 0.7 mg/day ZnCl2 for two months before mating and till to the day 5th of pregnancy, the females of this group were mated with males dosed 0.7 mg/day ZnCl2 for two weeks before mating; T2, dosed 0.7 mg/day ZnCl2 for two months before mating and till to the day 16th of pregnancy and then were mated with control males (not exposed to any level of ZnCl2); T3, dosed 0.7mg/day ZnCl2 for two months before mating and till the end of pregnancy and were mated with control males;

The histological study of the pecten oculi of falcon eyeball is consists from 11-12 pecten plates connected to the pectin bridge. The pectin oculi extending from optic nerve toward the virtous body. Its characterization are highly visualization and continuous capillary network which infiltrated by melanocyte. The pecten oculi without muscles and nerve fibers. The study revealed that cornea of falcon eyeball was characterized by fine curvature, translucent with peripheral thickness. The cornea occupied the quarter of fibrous tunica. It consists from five layers: - anterior corneal epithelium, Bowman's membrane, stroma, Descement's membrane and endothelium.

The effect of 1% methylene blue solution was investigated in this experimental trial for prevention and/or reduction of intra-abdominal adhesion formation, following induction of conventional laparotomy, by postmortem and histopathological confirmations, in a rabbit model. Twenty adult, male, local breed rabbits were used in the study. They were allocated randomly and equally into 2 groups; Control group (CG) and Treatment group (TG). All were subjected to mid-ventral conventional laparotomy under the effect of general anesthesia, and the ascending colon was exteriorized and its serosal and subserosal layers were mechanically abraded by a dry sterile soft toothbrush. In TG, 2ml of sterile 1% methylene blue was applied intrapretonially pr

Behavioral problems are very important because they are one of the most common reasons for the relinquishment of cats. In this study, the frequency of behavioral problems in 167 cats attending Small Animal Hospital, Faculty of Veterinary Medicine, University of Tehran, was evaluated with the use of a questionnaire. Interaction with other animals and people, outdoor access, breed, and sex of the cats were evaluated for association with the occurrence of the behavioral problems. Aggression towards people and other cats, Elimination problems, Scratching objects, Fearfulness, Attention seeking, Excessive grooming, Chasing small animals, Hiding, Vocalization in owner's absence, Obsessive behaviors and Eating fiber material were the behavioral

Forty seven blood samples were collected from local house cats 25 male , 22 female , ranging in age from 1-15 year for both sexes for measuring the following parameters coagulation tests (total Platelets count, Clotting time, Prothrombin Time & Fibrinogen concentration ) and proteins concentration tests (Total protein concentration, Albumin , Globulin concentration ) and iron and copper concentration tests .the results as were follow (228.5 –1,537) X 109\L , (2-5)min , (6 - 15) sec , (0.5 -4) g\L , ( 61 - 83 ) g\L , (23.1 -38.2) g\L , (25.9-54.2) g\L , (5.21 – 45.56) μmol/L , (2.29 - 36.52) μmol/L) .the result showed significant differences at level (P<0.05) between the male and female of local house cats in total Platelets

The objective of this study is the identification of inorganic elements in egg shell of some wild birds including House Sparrow, White- eared Bulbul, Collared Dove and Rock Dove. Samples of eggs from these birds were collected from Baghdad city during 2011. Egg shell were analysed for ash, macro-elements and micro- elements including Ca, P, Mg, Fe, K, Mn, B, Zn, Co, Cr and Pb. The findings revealed that the ash percentage was high in egg shell of all birds under study which were consecutively 1.73, 1.72, 1.79 and 1.78 % for House Sparrow, White- eared Bulbul, Collared Dove and Rock Dove, Ca percentage was the highest percentage among the other elements which were 97.3, 97.4, 97.8 and 97.8 % respectively, where as P and Mg were ranging be

The goal of this study was to isolate and characterize a complete set of phages that are
active against Escherichia coliserogroups O1, O2, and O78, the main causative agents of
avian colibacillosis. A mixture of E.coli (O1:K1), (O2:K1), and (O78:K80) used as host to
isolate phages from wastewater and fecal samples from poultry processing plants. Eleven
phages were isolated, only two of them EC-NJ4 and EC-NJ7 were selected for further
characterization. EC-NJ4 and EC-NJ7 had icosaheadral heads, necks and contractile tails,
with tail fibers and therefore belonged to Myoviridae, with genome sizes of 67.06 – 68.04 kb
and they lysed 100% of serotype O1, O2, and O78. The two phages were resistant to pH 5-9,
and phage E

Sixty cows were included in this study which was conducted in Baquba for the period from April 2011 to October 2011. They were clinically diagnosed as having bovine ephemeral fever (BEF) infection. Blood samples were collected from infected cows before, after treatment, and after recovery. Additionally, 30 healthy cows were included as control group. Sera were separated and submitted for the determination of phosphorus, glucose, cholesterol and pH levels. Statistical analysis of biochemical values revealed significant decrease in serum cholesterol levels in infected cows as compared to control group (P<0.05). Furthermore, the diseased cows showed low levels of pH (acidosis), thus when they treated by intravenous injection of 5% sodium

Sixteen normal colostrum-fed -Friesian and Holestein-Friesian X Angus crossbred calves, ranging in age from 2 to 7 weeks were divided into 4 vaccinated groups and 1 nonvaccinated (control) group. Group I consisted of 2 calves vaccinated orally twice at a two week interval with small doses of organisms ( 104 thar1l05 live virulent Salmonella gghimurium, strain 108-11). Group ll consisted of 2 calves vaccinated orally twice at two weeks interval with 1011 organisms (modified Salmonella typhimurium, strain SL 3261 ). Group [[1 consisted of 2 calves vaccinatedlivitwice at two weeks interval with small doses of organisms ( 104 than 105 virulent Salmonella typhimurigg, strain 108-11). Group IV consisted of 3 calves which were vaccinated 1M twi

Experimental induction of vitamin E and selenium deficiency by deficient diet was carried out on Awassi ewes and their newborn lambs. The clinical signs were characterized by sudden death in 4 lambs out of 14 lambs in deficient group and other lambs showed a variable signs included inability to suckle, arched back, weakness, dullness, emaciation and recumbency. Serum selenium and vitamin E levels of these lambs were 0.01 ppm and 0.34 mg/L respectively. The clinical signs in ewes included loss of body weight and loss of wool, Weakness, dullness and recumbency. Serum selenium and vitamin E levels of these ewes were 0.02 ppm and 0.61mg/L respectively. It was concluded that vitamin E and selenium are essential antioxidants and their deficien

In order to achieve precise diagnosis of toxoplasmosis in suspected cases of aborted women and infants with abnormal clinical manifestation, 1725 serum samples consisting of 1450aborted women and 77 infants were tested by enzyme linked immune sorbent assay (ELISA), using IgG, IgM and IgA platellat oxogondii kits, 865 (56.64%) were found positive, 848 ( 58.48% ) were found in aborted women and 15 (22.07%) infants with a significant differences (P≤ 0.05).The immunoglobulin IgM , were detected in 72 (8.49%)cases of aborted women, IgM with IgG were found in 44 (61.11%) and with IgA in two (2.77%) cases ,whereas found in three(17.64%)cases of infants. While IgA were found in 77(9.08%) cases of aborted women, and 6 (35.22%) infants. IgG was

Terbinafine is a fungicide given orally with the dose of 100 mg/kg body weight per day for each ewe for 60 days. The vaginal smears of ewe, body and ovarian weight were daily administered at slaughter time; ewes were slaughtered at 60th day. Estrous cycle was affected by showing a significant reduce in the estrous cycle length of each phases of estrous cycle with associated significant increase in the diestrus phase in terbinafine treatment as compared with control group (olive oil treatment as adjuvant) of ewes. There was a significant reduce in the number of follicles and a significant raise in the number of atretic follicles in treated group as compared with control group as well as upsurge the progesterone/estrogen ratio. The body an

Thirty six trials of flushing with riched Delbeco`s phosphate buffer media applied in this study using a plastic two ways folly catheter, introduced by metal robe inside the cervix of donor cows and by air bubble fixed it in the cervical canal in 3 positions, one near the cervico-vaginal opening or at the first third of the cervical canal, the 2nd one at the middle third of the cervical canal and the 3rd one near the utero-cervical opening or at the last third of the cervical canal. The process of flushing was carried out after the fixation of the folly catheter in the three sites and the number of embryos collected by each collection site.
The results showed that the number of embryos detected in the third method where the fixation w

Out of 210 tissue samples (lung and lymph nodes ) collected from buffaloes examined by ELISA specific to(BVDV) Bovine Viral Diarrhea Virus antigen ten (10) samples were positive out of 160 lung tissue collected with (4.7%)and (3)positive sample were detected out of 50 lymph node tissue samples collected with (1.4%). Totally 13 positive samples with (6.1%) in buffaloes examined. The total positive tissue samples were divided to three age group. Group age one from (birth -6 month), group age two from (6-18 month) and group age three (18 month up). The positive samples were (1),(10) and (2) with (0.47%),(4.7%)and (0.95%)respectively that indicate the presence of BVD antigen in buffaloes (Bubals bubalis) .Out of (210) sera samples collected

    This study was designed to explore the effect of the ethanolic extract of Sonchus oleraceus on Escherichia coli infection in mice. The LD50 of the orally dosage ethanolic extract of Sonchus oleraceus was found to be 800gm/kg B.W for two weeks. Twenty five mice were randomly divided into five groups. The first group was given orally the ethanolic extract with dose 800 mg/kg B.W for two weeks, then infected with Escherichia coli bacteria with dose 1×10⁸ cfu for ten days. Second group was infected with Escherichia coli bacteria with dose 1×10⁸ cfu for ten days then treated with ethanolic extract of Sonchus oleraceus for two week

The study was carried to investigate the pathological effect of lyophilized antigen of Trichophyton mentagrophytes in mice. Fifty mice were divided into three groups. The first group 20 mice were immunized subcutaneous (s/c) with 0.5 ml of T.mentagrophyte antigen 20 µgm/ml, by two doses, 14 day intervals, between them. The second group 20 mice and third group 10 mice considered as positive and negative control groups respectively. After 30 days post immunization first and second groups were challenged intradermal I/d. with 0.1 ml of fungal suspension contain (1×107 ml) of viable virulence T.mentagrophyte while the third group injected intraperitoneally I/P. with 0.5 ml of sterile phosphate buffer saline. All mice of the first and secon

Adult female rats were given a zinc deficient diet for six weeks. During this period their ovaries were compared with those of» normally fed rats. The histochemical activities of two zinc metalloenzymes, namely alkaline phosphatase (APase) and carbonic anhydrase (CA) were markedly lowered three and two weeks after starting feeding, respectively. lg vitro, reactivation to the control level of activity of both enzymes by exogenous zinc added to the incubation medium suggests absence of zinc from the apoenzymes-is the cause of decreased activity rather than decreased synthesis of the apoenzymes.

Total sewage of the middle-sized town in our country having different local industries of v

Specific-pathogen-free males Balb/c and C57 Black mice were inoculated intraperitoneally wi

      The objective of this study was to investigate the percentage of male and female ovine embryos produced after oocytes fertilization by sperm selected with self-migration (swim-up) technique. The sex of in vitro produced embryos was determined by Polymerase chain reaction (PCR). results indicate that the fertilization by sperm selected using Swim-up technique was 81.8%  (9/11) male and 18.1% (2/11) female in vitro produced embryos when centrifuged at 200×g, while centrifugation at 300×g  was 72.7% (8/11) male and 27.2% (3/11) female embryos. It was concluded that the use of “swim-up” technique refer to significant (P<0.05) occurred for increasing male embryos ratio compar

It is a custom among among horse breeders in Iraq to service the mares at first

Biochemical values for fifty clinically healthy arabian camels of both sex were determined for the normal

A comparative study on air drying of human erythrocytes for scanning electron microscopy (S E

A study on the epidemiology  of helminths of buffalloes, conducted at two different areas, revealed the followings;

The postulation that circulating lipophages in cholesterol-fed guinea pigs may originate from lipidladen splenic

This preliminary radiographic study was performed with the available instruments to describe the arterial blood supply

Out of 704 sheep examined from three provinces (Baghdad, Hilla and Diala) in the central part of Iraq, 323(45.88%) were infected with Theileria  hirci, 80 (11.36%) with Anaplasma ovis and 19(2.70%) with Babesia motasi. The percentage of sheep infected with Th. Hirci was much higher (P < 0.01) than those with A. ovis and B. motasi. The rate of infection with Th. hirci and A. ovis but not B. motasi increased with advancing age. There was no significant (P>0.05) difference in the rate of infection between males and females