To evaluate the effect of different hormonal treatments on reproductive performance of local goats, 60 adult does were synchronized by using sponges impregnated with 40 mg medroxy acetate (MAP) for 14 days. Following sponges withindrawn, the does were allocated randomly to four equal groups. The first group was served as control and intramuscularly injected with normal saline (T1). The does in group 2,3 and 4 were injected intramuscularly with 250 i.u hCG(T2) 400 i.u. eCG(T3) and 20 µg GnRH(T4), respectively. Results revealed that the highest estrus response was recorded in T2 (100%) followed by T4(93.3%), T1 (86.7%) and T3(73.3%). Fertility rate was significantly (p˂0.05) higher in T4 (93.3%) and the lowest was found in
... Show MoreTrichoderma harzianum produced 5.383 U/mg of laccase in solid state fermentation (SSF) using 10gm of wheat bran as substrate with moisture content 1:0.7 w/v, using 4 plugs×5mm as inoculums size, for 7 days at 28C°. Laccase was partially purified with 7.260 fold and yield 84.01% by ion exchange chromatography DEAE-cellulose. Partially purified laccase had an optimum pH of 4 and was stable in pH range from 3.5 to 5.5. The optimum temperature for laccase activity was 35C° and stability was range from 15 to 35C° with 1h of incubation. The ability of T. harzianum isolate to decolorize textile dyes on solid media appears completely decolorization of Blue dye with concentrations 50,100 and pa
... Show MoreThe PRNCR1 gene variant among sample of Prostate Cancer (PCa) Iraqi patients case-control study was used, with PCa patients (n=32) and healthy individual control (n=33). It was successfully identified two SNPs in prostate cancer-associated noncoding RNA-1 (PRNCR-1) gene by using PCR technique and direct sequencing . The first SNP is rs13252298 (A>G) have three genotypes (AA,AG,GG )and the second SNP is rs5013678 (T> C) have three genotypes TT,TC,CC),these genotype frequencies were not in agreement with Hardy-Weinberg Equilibrium (HWE) in patients and controls .It was observed that AG genotype was more frequent in patients than controls(
This study examined the potential association between polymorphisms of Caspase 9 −293del (rs4645982) and Caspase8−652 6N ins / del (rs3834129) and the danger of acute and chronic myeloid leukaemia in Iraqi population. This case-control study was conducted as 128 (62 acute, and 66 chronic) myeloid leukaemia patient and 65 healthy individuals not infected with cancer. A conventional PCR method was used for detection in this study. The results showed that the Caspase9 −293del (rs4645982) in
... Show MoreThe present study was carried out to detect efficacy of essential oils (oregano oil, anise oil, citrus oil and Digestarom® P.E.P) as an antiviral on Newcastle disease infection in broilers.One hundred twenty, one-day-old broiler chickens (Copp500) were used. On day twenty, the one hundred chickens were divided into six groups.Each group contained 20 birds. All chicks were challenged with median) virus titer embryo infectious dose (EID50) 1ml per bird intraocular, intranasal and orally of the isolated very virulent Newcastle disease virus that identified by (HA, HI test, PCR and ICPI) and treated with the essential oils blend level used in drinking water for five days after challenge, G1 oregano
... Show MoreThe present investigation was carried out to investigate the antibacterial efficiency of silver nanoparticles (AgNPs) in vitro against Aeromonas hydrophila using disc diffusion assay and minimum inhibitory concentration. The pathogenic A. hydrophila was isolated from infected common carp, usually diagnosed by chemical methods, and Avitek 2 compact device were used to confirm the diagnosis. The effectiveness of the prepared AgNPs was tested by chemical and biological (green synthesis using lemon extract) methods and were diagnosed by Fourier-transform infrared spectroscopy (FTIR), UV-Visible spectroscopy, Transmission electron mi
... Show MoreThe current study was designed for analysis of the pattern of the nucleotide sequence of the tissue DNA isolates based on the ITS2,28SrDNA gene by the traditional polymerase chain reaction. All amplicons were well suited for the prepared primer with size 873 bp . and identical ratio ranged 87.2- 98% of the same species, as included a high similarity of the isolates taken camels in Iran and Iraq related to Parabronema skrjabini . Phylogenic tree inferred the degree of relatedness between 28SrDNA sequences deposited in the international nucleotide bank sequence database (NCBI). The sequence of Parabronema skrjabini was recorded in the Genbank under accession number MT742154.1.
This study was carried out at University of Baghdad - College of Agricultural Engineering Sciences - research station B during the fall season of 2019-2020, in order to evaluate the effect of Ozone enrichment and the foliar application of organic nutrient on nutrient and water use efficiency and fertilizer productivity of broccoli plant using the modified NFT film technology. A factorial experiment (2*5) was carried out within Nested Design with three replicates. The ozone treatment was distributed into the main plots which consisted of oxygen (O2) and ozone (O3). The foliar application of organic nutrients were distributed randomly within each replicate including five treatments, whic
... Show MoreExtraction of Copper ion from its water solutions using plant waste (compost), sludge and Bentonite metal as cheap natural adsorbed materials. It was found that Copper ion extraction was based on concentration of Copper and amount of the adsorbed materials. Highest amount of adsorption was obtained at 180 µg Cu ml-1 as Bentonite, plant waste and sludge respectively. Thermal equilibrium of the system was studied by Langmuir equation, R2 gave a good linear relationship in succession. Maximum regulatory capacity and regulatory capacity at equilibrium is 1.0 , 0.99 and 0.96 to plant waste, sludge and Bentonite respectively, as the preference coefficient
... Show MoreA field experiment was carried out at the field with clay loam soil in the winter seasons of 2018 - 2019 and 2019 - 2020 in order to study the effect of three levels of salt stress on yield and its components for four cultivars of barley (Hordeum valgare L.(. which be approved in Iraq.Factorial experiment with in Randomized Complete Block design with three replications. The treatments included three levels of irrigation water salinity (5,10,15) ds.m-1 and four cultivars of barley (IPA 99, Buhooth 265, Samir, and Amal). The results showed that the salinity of the irrigation water caused a significant decreases of the number of spikes.m-2, number of grains. spike
... Show MoreThis study was aimed to detect the spreading of E.histolytica among children with diarrhea, to achieve that,221 stool specimens were collected from diarrheal children of all ages and both gender, in Baghdad city at a period extended from early September 2019 to the end of February 2020. The collected specimens were examined directly by the light microscope for detecting the presence of E.histolytica. DNA was extracted from positive microscopically stool specimens, then<
... Show MoreIn this study out of 130 different clinical specimens, 70 Acinetobacter baumannii were collected from hospitalized patients. Then; all the isolates were characterized using biochemical tests and confirmed by using the Vitek II compact system. All isolates of Acinetobacter baumannii were subjected to the disc diffusion method to determine it's a susceptibility to 8 types of antibiotic disc, and the result showed these bacteria were resistant to Cefepime, Imipenem, trimethoprim, Piperacillin, tetracycline, Amikacin, ampicillin-sulbactam and levofloxacin. According to bacterial susceptibility test, all isolated were showed highly resistance to Trimethoprim (88.57%) and the lower resistance to tetracycline
... Show MoreForty nine sputum specimens were collected from patients with aspergillosis attended to four hospitals in Baghdad. The frequent species of Aspergillus identified included Aspergillus fumigatus 23(46.9%), Aspergillus niger 14 (28.6%), followed by Aspergillus flavus 12 (24.5%). According to age group factor, the age group (50-59) years appeared to be more susceptible to infected by aspergillosis with percentage at (24.5%). The results was revealed that no significant differences between male and female with aspergillosis infection. To detect A.fumigatus isolates by molecular methods, the genomic
... Show MoreThis study was aimed to measure marketing efficiency and study important factors affecting , using TOBIT qualitative response model for wheat crop in Salahalddin province. Results revealed that independent factors such as (marketing type, crops duration in the field, average marketing cost, distance between farm and marketing center, and average productivity) had an impact on wheat marketing efficiency. This impact varied in size and direction due to value of parameters. Values of marketing efficiency fluctuated within cities and towns in the province. The average value on the province level was 76.75%. This study was recommended developing marketing infrastructures which is essential to efficiency increases. In addition, it is i
... Show MoreA field experiment was conducted on a gypsiferous sandy clay loam soil to examine the effects of blending Triple superphosphate (TSP) with urea on N, P concentrations in plant and growth parameters of broad bean. The experiment was a factorial randomized complete block design (RCBD) with three replicates. The first factor was type of application as briquettes which include T1 (one layer of TSP between two layers of urea) and T2 (one layer of urea between two layers of TSP), the second factor was application depth (5 and 10 cm D1 and D2), and the third factor was application rate (1.0, 1.25, and 1.50 as much as N and P fertilizer recommended for broad bean, R1, R2 and R3). Broad bean was planted and the following growth parameters
... Show MoreThe phytochemical analysis of Azolla filiculoides Lamark in the methanolic whole plant extract was undertaken and published for the first time in Iraq in this article. The existence of twenty-four bioactive phytochemical compounds were discovered using GC-MS analysis. , such as Ethyl Formate, 1,3-Cyclohexanedione, Butanal, 2-Nonenal,9-Octadecenal,2,4,6-Trimethyl-1-nonene, 3-(Prop-2-enoyloxy) tetradecane, Carbonic acid, 9-Oxabicyclo [6.1.0] nonane. Oleic Acid, 13-Docosenoic acid, Z-10-Pentadecen-1-ol, Oxirane, Diethyl carbonate. Hydroperoxide, 2-Butene ozonide. These various active phytochemicals of alkenes, alkanes, esters, ethers, and carboxylic acids were found in the plant with great potential to bind with
... Show MoreThis study was conducted at the Animal |Production Sciences, College of Agricultural Engineering Science, University of Duhok to estimate the effect of different levels of oleobiotec on the production performance, egg quality of Japanese quail. A total of 105 Japanese quail 17 weeks of age obtained from a local farm and distributed into five treatments with three replicate for each one had (7 quail). Different doses of oleobiotec significantly (P ≤ 0.05) improved egg production, egg weight, and egg mass, fed conversion ratio and egg weight uniformity percentage. Concerning egg haugh unit significantly improved (P<0.05) by using oleobiotec while, other egg quality did not influenced with different level of ol
... Show MoreThis study was aimed to development and modification the method of analysis for active ingredient in Tamsulosin drug by using high performance liquid chromatography (HPLC) also determined shelf life and storage conditions for Tamsulosin. Chromatographic conditions utilized stationary phase C18 (250*4.6) mobile phase 0.05Nmixture of di hydrogen ortho phosphate and Acetonitrile 55:45 , preserve the flow rate near (1ml/min) and length of wave has been 275nm, The retention time found to be 10 minute for the HPLC process . The Tamsulosin assay result was found to be 99.93% . The calibration curve linearity analysis results showed a strong linear relationship in the concentration range (10-
... Show MoreThe aim of this study was to establish the existence and interaction of TMPRSS2 – ERG gene fusion status with clinicopathological features of prostate cancer patients. This research consisted of 123 embedded formalin-fixed tissues obtained from the prostate tumor patients. The above gene fusion is detected through the technique of fluorescent in situ hybridization (FISH) by means of a triple color probe. Seven samples have not been scored due to technical difficulties and 46 patients have fusion (39.6%), while the remaining (70) have not been seen with fusion. Of the 46 fusion-positive, 17 (36%) were caused by ERG-translocation, of the other 29 (63%) were caused by the interstitial segment deletion between the two gene
... Show MoreThis study was aimed to detect the prevalence of blaVIM and blaNDM genes among Enterobacter cloacae isolates that were isolated from Iraqi patients. About 50 bacterial isolates were collected from different hospitals in Baghdad city and all these isolates were diagnosed using biochemical tests and CHROMagar culture media and conformed using the Vitek II system. The antibiotic susceptibility for E.cloacae isolates was determined using the disk diffusion (Kirby Bauer) method and the results showed that these bacteria were showed resistance to Cefepime and Meropenem antibiotics in percentage (50%), (40%) respectively. In addition it is resistant to Amikacin, ampicillin/sulbactam, Piperaci
... Show MoreThis study was carried out in the poultry field in Jadiriyah, affiliated with the Department of Animal Production / College of Agricultural Engineering Sciences - University of Baghdad. This experiment used 480 one-day-old, unsexed broiler chicks of the broiler breed (Ross-308) with an average weight of 39.8 g to investigate the effect of adding different levels of turmeric root (CURCUMA LONGA) (CURCUMA LONGA) powder to the diet on some characteristics and physiology of commercial broilers exposed to heat stress. Chicks were distributed randomly and equally between 4 treatments (120 chicks/treatment), and each treatment included 3 replicates, at a rate of (40 chicks/repeat). Chicks were fed on the starter, growth and fin
... Show MoreThis study was aimed to improve the rheological and qualitative properties of the most common bread, wheat bread, by barley pentosanes, which is not optimally utilized although it's many benefits and its huge production. Therefore, water-soluble pentosanes (WSP) and water-insoluble pentosanes (WIP) were extracted from barley (Hordeum vulgare) and added to wheat flour at different percentages. Five treatments were prepared: (T1) from flour only, (T2 and T3) adding WSP at levels(1 and 2%), (T4 and T5) adding WIP at the same levels respectively. The results showed significant improvement in rheological and qualitative properties of the dough and bread made from this dough with increasing the level of addition. The wat
... Show MoreThis study objective was aimed to prepare a suggested conception for the quality of some elements of the extension service for vegetable growers from their point view at Baghdad Governorate. In order to achieve the objective , a scheme was prepared consisting of (20) criteria distributed into (4) areas: sources of service provision, service timing, topics and content of the extension services, service providers. Data were collected by the random sample of 108 respondents vegetable growers, representing 30% of the agricultural divisions. In the two directorates of agriculture in Baghdad Governorate , Al-Karkh , Al-Rusafa. The results of this research concluded that each of the areas and criteria of the proposed perception obtained
... Show MoreThis study was aimed to investigate combining ability among maize inbred lines. Randomized complete block design with three replicates was used to study the general combining ability of parents, and specific combining ability of 12 F1s. Combining ability was studied in a partial diallel cross in maize for traits yield and attribute at two locations (Qlyasan and Kanipanka) at Sulaimani Governorate. The cross 3×6 produced the highest value kernels yield and most of the attributed traits as the average of both locations. Mean squares due to crosses, GCA and SCA were highly significant for most traits as the average of both locations. The ratio of σ2gca/ σ2sca was found to be less than one for all traits exce
... Show MoreThis experiment was conducted in order to determine the effect of spraying acid salicylic (SA) at concentrations of 250, 500 mg L-1 and hydrogen peroxide H2O2 at 3% and 6% compared with water as a control treatment on three cultivars of date palm tree (Barhi, Hilali and Majhoul), which are propagated via tissue culture under salt stress conditions, to estimate the gene expression of Superoxide dismutase (SOD), The SA 250 and 500 mg L-1 for Folding values achieved the highest gene expression for the cultivar Barhi and Hilali, which reached 2.549121, 3.363586, 5.098243, and 4.924578, respectively, While the highest gene expression of this enzyme for the Majhoul when treated with 6% hydrog
... Show MoreThis study was carried out to evaluate the micrografting success of Red globe cultivar (Vitis vinifera L.) on three rootstocks Ramsey, Freedom and SO4 and investigate the effect of some factors on the micrografting success. Furthermore, compatibility in the graft union was studied. The results showed that Red globe on un-rooted Freedom achieved the highest percentages of scion survival (100.00 %), scion bud burst (60.00%), graft union formation (60.00%), and rooting (40.00%), also longest shoot (2.00cm), highest root number (8.00) and root length (4.50cm). Murashige and Skoog (MS) medium Supplemented with Indole Butyric Acid (IBA) at 1mgL-1 achieved the highest percentages of scion survival (95.20%), scion bud burst (6
... Show MoreThis study investigates the efficacy of sand coated with calcium / aluminum (Ca/Al)-layered double hydroxide (LDH) in the elimination of methyl orange dye from simulated groundwater using method of permeable reactive barrier. For green (sustainable) manufacturing of this sand, aluminum prepared from dissolution of alum (cheap coagulant) can be interacted chemically with calcium extracted from cement kiln dust byproduct to create nanoparticles of Ca and Al that precipitate on the filter sand. Sorption measurements for interaction of methyl orange dye and coated sand was well formulated by Langmuir model with highest value of correlation (determination coefficient= 0.999) and lowest errors (sum of squared errors= 0.0122). Also, the
... Show MoreThis study was carried out to investigate thermal treatments, immersing in black seed and flaxseed oils on postharvest quality pomegranate fruits cv. Salakhan . Fruits were randomly divided into two experiments: First, thermal treatment by which fruits are immersed for four minutes at 25, 40 and 50°C in hot water (HW), and fruits are exposed to hot air (HA) for one hour at 40 and 50°C plus control treatment. Second, fruits are immersed for 4 minutes in 1 and 2% for the black seed and flaxseed oil, plus control treatment. Then, fruits are placed in the storage at 5±1 ºC and 85-95% of relative humidity for four months. Both experiments performed by using Complete Randomize Design. The results were indicated that soaking f
... Show MoreThis study was aimed to inhibition of purified collagenase produced from S. aureus by local plants inhibitors (fruits and leaves). Sixty two local isolates of S. aureus which were isolated and identified by biochemical test subjected to the primary and secondary screening processes to select the active S. aureus isolate for collagenase production. Among these isolates, forty nine isolates with maximum zone of hydrolysis ability in primary screening (the ratio of Z/G more than 10 mm) were selected for secondary screening. It has been found that S. aureus HN77 had the highest productivity of the enzyme (49.122 U/mg protein). The selected isolate with highest leve
... Show MoreThis experiment was aimed to investigate the Toxicopathological effect of N, N-Dimethylformamide (DMF) (1/10LD50) on the adult male rats' liver. Sixteen adult male rats were used in this experiment. After acclimatization for two weeks, they were divided equally into two groups as follows: Group-C-control: Normal diet and water for 30 days (n=8 rats).Group-T1: DMF 645.5mg/kg. B.W as 1/10LD50 for30 days ( n=8rats ) , after thirty days collected blood for liver enzymes analysis then all animals killed at the end of the experiment and the liver samples saved at 10% formalin for histopathological preparation. The results showed that serum liver enzymes (ALP, ALT, and AST) concent
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